当前位置： SCI文献检索 > DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE期刊下所有文献 > Pleural effusion with negative culture: a challenge for pneumococcal diagnosis in children.

Pleural effusion with negative culture: a challenge for pneumococcal diagnosis in children.

Abstract：

BACKGROUND:Pneumococcal parapneumonic effusion seems to be increasing in children in the postvaccine era and is frequently associated with negative culture. Due to the low yield of culture, culture-independent tools are evaluated. METHODS:Culture-negative pleural fluid specimens from 38 children with parapneumonic effusion were examined for pneumococcal lytA by quantitative polymerase chain reaction (qPCR) and soluble antigen (C-polysaccharide) using an immunochromatographic test (BinaxNow Streptococcus pneumoniae). RESULTS:In 81% (30/37) and 63% (24/38) of the specimens, a positive result was obtained by qPCR and antigen detection, respectively. Most mismatches were observed in specimens with low quantities of pneumococcal DNA and a negative antigen test. CONCLUSIONS:Our results suggest an imperfect relationship between the 2 described methods. The immunochromatographic assay is a simple diagnostic tool, which can be used when resources are limited, and even after antibiotic use, but negative results may require confirmation through a more sensitive test, such as qPCR.

journal_name

Diagn Microbiol Infect Dis

journal_title

Diagnostic microbiology and infectious disease

authors

Perez VP,Caierão J,Fischer GB,Dias CA,d'Azevedo PA

doi

10.1016/j.diagmicrobio.2016.07.022

subject

Has Abstract

pub_date

2016-10-01 00:00:00

pages

200-4

issue

2

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(16)30230-9

journal_volume

86

pub_type

杂志文章

相关文献

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE文献大全
  • Antimicrobial activity of ceftobiprole, a novel anti-methicillin-resistant Staphylococcus aureus cephalosporin, tested against contemporary pathogens: results from the SENTRY Antimicrobial Surveillance Program (2005-2006).

    abstract：:Ceftobiprole is a 1st-in-class anti-methicillin-resistant Staphylococcus aureus (MRSA) extended-spectrum cephalosporin currently in clinical trials for the treatment of complicated skin and skin structure infections (cSSSIs) and nosocomial pneumonia. This agent is also active against other prominent Gram-positive and ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2008.02.008

    authors： Fritsche TR,Sader HS,Jones RN

    更新日期：2008-05-01 00:00:00

  • Are central venous catheter tip cultures reliable after 6-day refrigeration?

    abstract：:Present guidelines recommend culturing only central venous catheter (CVC) tips from patients with suspected catheter-related bloodstream infection (CR-BSI). However, a high proportion of these suspicions are not confirmed. Moreover, CVC tip culture increases laboratory workload, and reports of colonization may be mean...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2009.02.011

    authors： Bouza E,Guembe M,Gómez H,Martín-Rabadán P,Rivera M,Alcalá L

    更新日期：2009-07-01 00:00:00

  • Interventional antimicrobial therapy in febrile neutropenic patients.

    abstract：:In febrile neutropenic patients, prompt empiric antimicrobial intervention is mandatory. Numerous studies have demonstrated the benefit of broad-spectrum beta-lactams active against Gram-negative aerobes as well as against streptococci and Staphylococcus aureus in this setting. With this interventional strategy, a red...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章,评审

    doi：10.1016/s0732-8893(99)00024-3

    authors： Maschmeyer G

    更新日期：1999-07-01 00:00:00

  • Comparison of five different susceptibility test methods for detecting antimicrobial agent resistance among Haemophilus influenzae isolates.

    abstract：:The detection of antimicrobial agent resistance among ninety-eight Haemophilus influenzae isolates was assessed by six different antibiotic test methods: agar dilution on Mueller-Hinton agar supplemented with 5% lysed horse blood (MH-LHB), E-test using both Haemophilus test medium (HTM) agar and chocolate Mueller-Hint...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(96)00026-0

    authors： Giger O,Mortensen JE,Clark RB,Evangelista A

    更新日期：1996-03-01 00:00:00

  • Use of boronic acid disk methods to detect the combined expression of plasmid-mediated AmpC beta-lactamases and extended-spectrum beta-lactamases in clinical isolates of Klebsiella spp., Salmonella spp., and Proteus mirabilis.

    abstract：:A study using boronic acid (BA), an AmpC enzyme inhibitor, was designed to detect the combined expression of plasmid-mediated AmpC beta-lactamases (pAmpCs) and extended-spectrum beta-lactamases (ESBLs) in bacterial isolates naturally lacking chromosomal ampC genes. A total of 122 Klebsiella spp., Salmonella spp., and ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2006.08.023

    authors： Song W,Jeong SH,Kim JS,Kim HS,Shin DH,Roh KH,Lee KM

    更新日期：2007-03-01 00:00:00

  • Commercial bacterial and fungal broad-range PCR (Micro-Dx™) used on culture-negative specimens from normally sterile sites: diagnostic value and implications for antimicrobial treatment.

    abstract：:The aim of this study was to evaluate the clinical value of partial 16S/18S rRNA gene sequencing with the commercial kit Micro-Dx™ used with the SelectNA™plus instrument on culture-negative samples. A retrospective study of microbiological and clinical data from a 2.5-year period was performed. Assessment of the clini...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2020.115028

    authors： Marbjerg LH,Holzknecht BJ,Dargis R,Dessau RB,Nielsen XC,Christensen JJ

    更新日期：2020-06-01 00:00:00

  • Detection of rubella immunoglobulin M from dried venous blood spots using a commercial enzyme immunoassay.

    abstract：:Confirmation of the diagnosis is critical to disease control in measles elimination and rubella control programs. In countries with limited infrastructure and laboratory capacity, collection, transport, and testing of venous blood samples may be difficult. We report the adaptation of a commercial enzyme immunoassay fo...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2005.06.017

    authors： Karapanagiotidis T,Riddell M,Kelly H

    更新日期：2005-10-01 00:00:00

  • Metabolic interactions of ciprofloxacin.

    abstract：:The mechanism and clinical relevance of the inhibitory effect of ciprofloxacin on the metabolism of selected drugs were studied in patients with bacterial infections. In study A, antipyrine tests were carried out in two groups of patients taking 1000 mg (group 1) and 250 mg (group 2) of oral ciprofloxacin for 7-10 day...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(90)90096-e

    authors： Ludwig E,Graber H,Székely E,Csiba A

    更新日期：1990-03-01 00:00:00

  • Corynebacterium jeikeium (group JK diphtheroids) endocarditis. A report of five cases.

    abstract：:Corynebacterium jeikeium is the name recently given to a group of coryneform rods, formerly known as the JK group, and associated with a wide range of serious infections. We describe five patients with C. jeikeium endocarditis observed in two Belgian hospitals over a period of 5 years. In three cases, the infection oc...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(89)90025-4

    authors： Vanbosterhaut B,Surmont I,Vandeven J,Wauters G,Vandepitte J

    更新日期：1989-05-01 00:00:00

  • Impact of AmpC overexpression on outcomes of patients with Pseudomonas aeruginosa bacteremia.

    abstract：:AmpC overexpression (AmpC++) is a significant mechanism of beta-lactam resistance in Pseudomonas aeruginosa, but its impact on clinical outcomes is not well established. To examine the influence of AmpC++ on clinical outcomes of patients with P. aeruginosa bacteremia, we screened all bloodstream P. aeruginosa isolates...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2008.11.007

    authors： Tam VH,Chang KT,Schilling AN,LaRocco MT,Genty LO,Garey KW

    更新日期：2009-03-01 00:00:00

  • Detection of herpes simplex virus in clinical specimens by DNA hybridization.

    abstract：:An assay to detect herpes simplex virus (HSV) DNA in clinical specimens has been developed. It utilizes nucleic acid hybridization with a 32P-labeled DNA probe prepared from a fragment of HSV DNA cloned in a plasmid vector. This assay can detect 5 X 10(4) plaque-forming units of cell-free HSV and as few as four virus-...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(83)90041-x

    authors： Redfield DC,Richman DD,Albanil S,Oxman MN,Wahl GM

    更新日期：1983-06-01 00:00:00

  • Detection of enterotoxin genes seg, seh, sei, and sej and of a novel aroA genotype in Jordanian clinical isolates of Staphylococcus aureus.

    abstract：:The presence of staphylococcal enterotoxin (SE) genes (seg, seh, sei, and sej) and the correlation of their prevalence with the genotypes were studied in 100 clinical isolates of Staphylococcus aureus. Polymerase chain reaction (PCR) of SE genes indicated that 39% of the isolates were enterotoxigenic. Thirty-seven per...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2006.04.002

    authors： El-Huneidi W,Bdour S,Mahasneh A

    更新日期：2006-10-01 00:00:00

  • Interferon gamma mRNA quantitative real-time polymerase chain reaction for the diagnosis of latent tuberculosis: a novel interferon gamma release assay.

    abstract：:The interferon gamma (IFN-γ) release assay (IGRA) is widely used as a diagnostic method for latent tuberculosis infection (LTBI). The QuantiFERON-TB Gold and QuantiFERON-TB Gold In-tube (QFT-IT) tests measure plasma IFN-γ levels using enzyme-linked immunosorbent assay (ELISA), and T-SPOT.TB counts IFN-γ-producing cell...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2012.09.015

    authors： Kim S,Kim YK,Lee H,Cho JE,Kim HY,Uh Y,Kim YM,Kim H,Cho SN,Jeon BY,Lee H

    更新日期：2013-01-01 00:00:00

  • Susceptibility testing of Clostridium difficile against metronidazole and vancomycin by disk diffusion and Etest.

    abstract：:A prospective study on the susceptibility of Clostridium difficile to metronidazole and vancomycin using the Etest and disk diffusion test was performed over a 6-month period. One hundred strains were tested; one strain was highly resistant to metronidazole (MIC = 64 micrograms/mL). The zone size of inhibition by the ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/s0732-8893(98)00139-4

    authors： Wong SS,Woo PC,Luk WK,Yuen KY

    更新日期：1999-05-01 00:00:00

  • Selection of Enterococcus faecium strains with stable and unstable resistance to the streptogramin RP 59500 using stepwise in vitro exposure.

    abstract：:Therapy of vancomycin-resistant enterococcal infections is an increasing problem for many large medical centers. One promising agent for use against Enterococcus faecium is RP 59500 (Quinupristin/Dalfopristin). To assess the potential for emergence of resistant strains during clinical trials with this new compound, we...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(96)00067-3

    authors： Millichap J,Ristow TA,Noskin GA,Peterson LR

    更新日期：1996-05-01 00:00:00

  • Evaluation of the efficacy of a posaconazole and anidulafungin combination in a murine model of pulmonary aspergillosis due to infection with Aspergillus fumigatus.

    abstract：:Posaconazole (PSC) in combination with anidulafungin (AFG) was evaluated in a murine model of pulmonary aspergillosis. Immunosuppressed animals were infected via the nasal cavity with 2 different A. fumigatus strains. The animals received PSC (oral, 20mg/kg per day) and/or AFG (i.p., 10mg/kg per day) for 7days. On Day...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2017.10.004

    authors： Bedin Denardi L,Pantella Kunz de Jesus F,Keller JT,Weiblen C,de Azevedo MI,Oliveira V,Morais Santurio J,Hartz Alves S

    更新日期：2018-01-01 00:00:00

  • Evaluation of Brucella dipstick assay for the diagnosis of acute brucellosis.

    abstract：:The diagnostic value of the dipstick assay was evaluated by comparison with Rose Bengal (RB), serum aglutination tests (SAT) and 2 mercaptoethanol test (2-ME) on consecutive serum samples submitted because of suspicion of brucellosis. Serum samples of 232 patients with suspected brucellosis that were submitted for lab...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/s0732-8893(02)00456-x

    authors： Altuglu I,Zeytinoğlu A,Bilgic A,Kamcioglu S,Karakartal G,Smits H

    更新日期：2002-11-01 00:00:00

  • Serotype distribution and antibiotic resistance of Streptococcus pneumoniae isolated from invasive infections after optional use of the 7-valent conjugate vaccine in Korea, 2006-2010.

    abstract：:This study examined the serotype distribution and antimicrobial resistance of pneumococcal isolates from invasive infections in children between 2006 and 2010, when the 7-valent pneumococcal conjugate vaccine (PCV7) was offered as an optional vaccine in Korea. Among 140 isolates collected from 8 centers, the common se...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2013.12.016

    authors： Cho EY,Lee H,Choi EH,Kim YJ,Eun BW,Cho YK,Kim YK,Jo DS,Lee HS,Lee J,Kim MN,Kim DS,Lee HJ

    更新日期：2014-04-01 00:00:00

  • Comparison of susceptibility results of anaerobic organisms determined by agar dilution method and Sceptor Anaerobe MIC/ID Micro Broth Dilution Panels.

    abstract：:A commercial broth microdilution system (Sceptor Anaerobe MIC/ID, BBL) for susceptibility testing of anaerobic bacteria was compared to a reference agar dilution system. Of the 172 organisms tested, only 7% failed to grow sufficiently for testing in the Sceptor system. In 1,590 antibiotic/organism combinations, 86.7% ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(87)90155-6

    authors： Hussain Z,Lannigan R,Schieven BC,Stoakes L,Groves D

    更新日期：1987-10-01 00:00:00

  • Urine D-arabinitol/L-arabinitol ratio in diagnosing Candida infection in patients with haematological malignancy and HIV infection.

    abstract：:Adult patients with hematologic malignancies along with HIV infected patients were prospectively studied to determine the performance of urine D-arabinitol/L-arabinitol (DA/LA) ratio in diagnosing invasive candidiasis. Ten evaluable febrile neutropenic patients had proven invasive candidiasis and elevated DA/LA ratios...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/s0732-8893(01)00310-8

    authors： Eisen DP,Bartley PB,Hope W,Sigmundsdottir G,Pehrson C,Larsson L,Christensson B

    更新日期：2002-01-01 00:00:00

  • Histoplasma capsulatum fungemia documented using the DuPont Isolator System.

    abstract：:A patient with disseminated histoplasmosis is described in whom blood cultures were negative using conventional media but positive for Histoplasma capsulatum using the DuPont Isolator system. Fungal blood culture methods are discussed. ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(84)90022-1

    authors： Wheat LJ,Bartlett M

    更新日期：1984-01-01 00:00:00

  • Role of quantitative CSF microscopy to predict culture status and outcome in HIV-associated cryptococcal meningitis in a Brazilian cohort.

    abstract：:This retrospective study aimed to evaluate the clinical, laboratory, and quantitative cerebrospinal fluid (CSF) cryptococcal cell counts for associations with in-hospital outcomes of HIV-infected patients with cryptococcal meningitis. Ninety-eight HIV-infected adult patients with CSF culture-proven cryptococcal mening...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2012.01.014

    authors： Vidal JE,Gerhardt J,Peixoto de Miranda EJ,Dauar RF,Oliveira Filho GS,Penalva de Oliveira AC,Boulware DR

    更新日期：2012-05-01 00:00:00

  • Discrepancies between in vitro activity of and in vivo response to antimicrobial agents.

    abstract：:The correlation between in vitro antimicrobial activity and the in vivo response to antimicrobial therapy is affected by multiple host factors, the site and nature of the infection, and the pharmacokinetics of the antimicrobial and its penetration into areas of infection. In certain instances, discrepancies are also c...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(83)90029-9

    authors： Washington JA 2nd

    更新日期：1983-03-01 00:00:00

  • Rapid methods in clinical microbiology. Clinical situations in which rapid methods may best be applied.

    abstract：:Utilization of rapid microbiologic methods could improve both the quality of patient care and help to reduce patient health care costs through more efficient patient management. Medically urgent situations such as meningitis, endocarditis, bacteremia, some soft tissue infections, ocular infections, and pneumonia in im...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(85)90047-1

    authors： Jorgensen JH

    更新日期：1985-11-01 00:00:00

  • Cytomegalovirus antibody detection in blood donors and mothers of very low birth weight neonates by using three serologic methods.

    abstract：:We compared three serologic methods for cytomegalovirus (CMV) antibody detection and determined the CMV antibody seroprevalence of blood donors and mothers of very low birth weight (less than 1250 g) neonates in the Greater Hartford region. CMV serology was determined for 577 healthy blood donors as well as for 147 mo...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(92)90035-r

    authors： Eisenfeld L,McLaughlin JC,Mayo D,Klevjer-Anderson P,Silver H,Krause P,Anderson J,Herson V,Savidakis J,Lazar AM

    更新日期：1992-02-01 00:00:00

  • In vitro activities of tigecycline against clinical isolates from Shanghai, China.

    abstract：:To evaluate the in vitro activity of tigecycline, the minimum inhibitory concentrations (MICs) of tigecycline against 1,201 strains of recent clinical isolates from 10 hospitals in Shanghai, China were determined and compared with selected comparators. Results showed that tigecycline had broad-spectrum antimicrobial a...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2004.08.007

    authors： Zhang YY,Zhou L,Zhu DM,Wu PC,Hu FP,Wu WH,Wang F

    更新日期：2004-12-01 00:00:00

  • Evaluation of three Copan viral transport systems for the recovery of cultivatable, clinical virus isolates.

    abstract：:Three Copan viral transport media (VTM) were compared to the BBL Viral Culturette and Micro Test M4 systems for their ability to maintain the viability of clinical strains of enterovirus, herpes simplex virus, adenovirus and parainfluenza virus. VTM were inoculated, incubated up to 72 h at both 4 degrees C and 22 degr...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/s0732-8893(02)00541-2

    authors： Dunn JJ,Billetdeaux E,Skodack-Jones L,Carroll KC

    更新日期：2003-03-01 00:00:00

  • New findings about trichodysplasia spinulosa-associated polyomavirus (TSPyV)--novel qPCR detects TSPyV-DNA in blood samples.

    abstract：:A new real-time PCR assay for trichodysplasia spinulosa-associated polyomavirus (TSPyV) DNA detection was designed, and blood samples from kidney transplant recipients and healthy individuals were screened. TSPyV-DNA was not detected in blood from healthy individuals, but 26.8% of kidney recipients presented TSPyV-DNA...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2015.10.011

    authors： Urbano PR,Nali LH,Bicalho CS,Pierrotti LC,David-Neto E,Pannuti CS,Romano CM

    更新日期：2016-02-01 00:00:00

  • Fusarium verticillioides fungemia in a liver transplantation patient: successful treatment with voriconazole.

    abstract：:Fusarium is an opportunistic fungal pathogen which is emerging as a significant cause of morbidity and mortality in immunocompromised hosts. We present a rare case of F. verticillioides fungemia that occurred in a patient who underwent a second orthotopic liver transplantation for chronic rejection and completely resp...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2011.08.024

    authors： Cocchi S,Codeluppi M,Venturelli C,Bedini A,Grottola A,Gennari W,Cavrini F,Di Benedetto F,De Ruvo N,Rumpianesi F,Gerunda GE,Guaraldi G

    更新日期：2011-12-01 00:00:00

  • Epidemiology and antimicrobial susceptibilities of wound isolates of obligate anaerobes from combat casualties.

    abstract：:Data from recent conflicts related to war wounds and obligate anaerobes are limited. We define the epidemiology and antimicrobial susceptibility of obligate anaerobes from Iraq and Afghanistan casualties (6/2009-12/2013), as well as their association with clinical outcomes. Susceptibility against eleven antibiotics (7...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2015.10.010

    authors： White BK,Mende K,Weintrob AC,Beckius ML,Zera WC,Lu D,Bradley W,Tribble DR,Schnaubelt ER,Murray CK,Infectious Disease Clinical Research Program Trauma Infectious Disease Outcome Study Group.

    更新日期：2016-02-01 00:00:00