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A diagnosis of Burkholderia pseudomallei directly in a bronchoalveolar lavage by polymerase chain reaction.

Abstract：

:Melioidosis is an infectious disease caused by the Gram-negative bacterium Burkholderia pseudomallei. Interest in the molecular identification of B. pseudomallei has increased after its classification as a category B agent by the US Centers for Disease Control and Prevention. The present article reports a diagnosis of B. pseudomallei directly in a bronchoalveolar lavage by polymerase chain reaction amplification. The results obtained show that direct detection of the 16-23s spacer sequence in bronchoalveolar lavage is a quick and specific test to diagnose melioidosis.

journal_name

Diagn Microbiol Infect Dis

journal_title

Diagnostic microbiology and infectious disease

authors

Couto MS,Cordeiro Rde A,Rocha MF,Grangeiro TB,Leitão Junior NP,Bandeira Tde J,Sidrim JJ,Brilhante RS

doi

10.1016/j.diagmicrobio.2009.05.010

subject

Has Abstract

pub_date

2009-09-01 00:00:00

pages

73-5

issue

1

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(09)00208-9

journal_volume

65

pub_type

杂志文章

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