The evaluation of a user-friendly lateral flow assay for the serodiagnosis of human brucellosis in Kazakhstan.

Abstract:

:Serum samples from all patients with culture-confirmed brucellosis including those with chronic disease from Kazakhstan tested positive in the serum agglutination test for titers > or = 1:25 and reacted in the Brucella immunoglobulin M/immunoglobulin G lateral flow assay (LFA) confirming the high sensitivity of these assays. The strong reactivity in the LFA observed for the majority (92.1%) of the samples from the patients with culture-confirmed brucellosis together with the user-friendliness of the assay procedure makes the LFA ideal for the confirmation of brucellosis in endemic areas in Kazakhstan. The Rose Bengal test lacked sensitivity in particular for patients with chronic brucellosis therefore limiting its value as a quick screening assay. The study emphasizes the importance of the LFA as a useful, rapid, and easy-to-perform tool in the diagnostic testing of brucellosis.

authors

Mizanbayeva S,Smits HL,Zhalilova K,Abdoel TH,Kozakov S,Ospanov KS,Elzer PH,Douglas JT

doi

10.1016/j.diagmicrobio.2009.05.002

subject

Has Abstract

pub_date

2009-09-01 00:00:00

pages

14-20

issue

1

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(09)00199-0

journal_volume

65

pub_type

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