Rapid qualitative antibiotic resistance characterization using VITEK MS.

Abstract:

:Development and standardization of simple, timely, and cost-effective antibiotic susceptibility assays are much needed to address the emergence of global resistance. The use of matrix-assisted laser desorption/ionization time of flight mass spectrometry is routine for bacterial identification. This study evaluated 2 assays using the VITEK MS for rapid detection and accurate differentiation of bacterial antibiotic susceptibility. We describe an extraction method and direct-on-target microdroplet growth assay (DOT-MGA). Non-susceptible and susceptible strains of Staphylococcus aureus, Enterococcus species, Escherichia coli, and Klebsiella pneumoniae were tested. The liquid extraction method and DOT-MGA proved to be reliable assays providing consistent differentiation between non-susceptible and susceptible strains. Results from this study support VITEK MS and these assays as rapid and accurate tools to augment traditional susceptibility testing. If implemented clinically, these assays can reduce the cost of patient care and the time to deliver critically needed treatment.

authors

Horseman TS,Lustik MB,Fong KSK

doi

10.1016/j.diagmicrobio.2020.115093

subject

Has Abstract

pub_date

2020-08-01 00:00:00

pages

115093

issue

4

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(20)30470-3

journal_volume

97

pub_type

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