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Use of and cost savings with morphologic criteria and the spot indole test as a routine means of identification of Escherichia coli.

Abstract：

:The use of primary isolation plate colonial morphologic criteria (CMC) of a flat, nonmucoid, lactose-fermenting, gram-negative rod on MacConkey agar and the spot indole (SI) test from the sheep blood agar plate was evaluated as a means for identification of Escherichia coli in comparison to kit (Micro-ID, API-20E) and conventional biochemical testing. In this preliminary phase of comparison of accuracy, 427 isolates of E. coli (69.8%) from a total of 612 isolates of lactose-fermenting gram-negative rods were evaluated. Of these E. coli isolates, 357 (83.6%) fit the CMC and were SI positive; 3 (less than 1% error rate) were not E. coli. In the second phase of the evaluation, using CMC and SI alone as a means for identification of E. coli, 472 (57.6%) E. coli isolates from a total of 820 Enterobacteriaceae isolates were assessed. Of these E. coli isolates, 326 could be identified using only CMC and SI (69.1% of the E. coli isolates and 39.8% of all Enterobacteriaceae isolates); 146 (30.9%) required additional biochemical testing because of atypical colonial morphology, because of the investigator's inability to differentiate colony types on both media or lack of isolated colonies on either of the two required media, or because as isolates from sterile body sites they were processed directly to Micro-ID kits. A minimum of 40% savings on Enterobacteriaceae identification schemes without compromising accuracy was calculated. As of November 1983, a direct (labor and materials) cost savings of approximately +200.80 per 100 Enterobacteriaceae identifications was projected.

journal_name

Diagn Microbiol Infect Dis

journal_title

Diagnostic microbiology and infectious disease

authors

Bale MJ,McLaws SM,Fenn JP,Matsen JM

doi

10.1016/0732-8893(84)90029-4

subject

Has Abstract

pub_date

1984-06-01 00:00:00

pages

187-91

issue

3

eissn

0732-8893

issn

1879-0070

pii

0732-8893(84)90029-4

journal_volume

2

pub_type

杂志文章

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