Description of a new Mycobacterium intracellulare pattern of PCR restriction enzyme analysis of hsp65 gene.

Abstract:

:This work comprises 9 pulmonary nontuberculous mycobateria isolates obtained from sputum of 4 different patients from Brazil. The sequencing and phylogenetic analysis allowed their accurate identification as Mycobacterium intracellulare. We report a mutation at position 453 creating a new HaeIII cutting site and, therefore, a new PRA-hsp65 M. intracellulare profile.

authors

Caldas PC,Campos CE,Dos Reis LM,Ferreira NV,de Carvalho LD,da Silva MV,Medeiros RF,Montes FC,Ramos JP

doi

10.1016/j.diagmicrobio.2014.01.028

subject

Has Abstract

pub_date

2014-06-01 00:00:00

pages

240-1

issue

2

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(14)00061-3

journal_volume

79

pub_type

杂志文章
  • Haemophilus influenzae acute endometritis with bacteremia: case report and literature review.

    abstract::Haemophilus influenzae rarely causes acute endometritis and the few published cases have always been associated with intrauterine devices (IUD). A 48-year-old female presented to the emergency department with a 3-day history of lower abdominal pain and fever. On physical examination she was tachycardic, hypotensive an...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章,评审

    doi:10.1016/j.diagmicrobio.2013.02.010

    authors: Martin D,Dbouk RH,Deleon-Carnes M,del Rio C,Guarner J

    更新日期:2013-06-01 00:00:00

  • Clinical and operational factors associated with treatment duration for cellulitis in outpatient parenteral antimicrobial therapy (OPAT).

    abstract::This study aims to identify factors associated with duration of intravenous (IV) and follow-on oral antibiotic therapy for cellulitis in patients treated through outpatient parenteral antimicrobial therapy (OPAT). A retrospective review of episodes of cellulitis treated over a year (January 2018-January 2019) at a lar...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2020.115305

    authors: Palit J,Cole J,Durojaiye OC

    更新日期:2021-01-06 00:00:00

  • Distribution of capsular and surface polysaccharide serotypes of Staphylococcus aureus.

    abstract::Because of its ability to cause serious and fatal infections, Staphylococcus aureus remains one of the most feared microorganisms. Methicillin-resistant S. aureus (MRSA) has long been a common pathogen in healthcare facilities, but within the past decade, it has emerged as a problematic pathogen in the community setti...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2007.01.016

    authors: von Eiff C,Taylor KL,Mellmann A,Fattom AI,Friedrich AW,Peters G,Becker K

    更新日期:2007-07-01 00:00:00

  • Pharmacodynamic profiling of cefepime in plasma and cerebrospinal fluid of hospitalized patients with external ventriculostomies.

    abstract::Population pharmacokinetic (PK) modeling and Monte Carlo simulation (MCS) were used to describe the pharmacodynamic profile of cefepime in the both plasma and cerebrospinal fluid (CSF). Plasma and CSF cefepime data were obtained from a PK study of 7 hospitalized patients with external ventricular drains. Concentration...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2005.09.007

    authors: Lodise TP Jr,Rhoney DH,Tam VH,McKinnon PS,Drusano GL

    更新日期:2006-03-01 00:00:00

  • Detection of Pneumocystis carinii by DNA amplification in human immunodeficiency virus-positive patients.

    abstract::The opportunistic pathogen Pneumocystis carinii (PC) is a frequent cause of a life-threatening pneumonia in human immunodeficiency virus (HIV)-infected individuals and in other immunocompromised hosts. Specimens obtained from 128 bronchoalveolar lavage (BAL) fluid samples from 123 HIV-positive patients with pulmonary ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 临床试验,杂志文章

    doi:10.1016/s0732-8893(97)00126-0

    authors: Savoia D,Millesimo M,Cassetta I,Forno B,Caramello P

    更新日期:1997-10-01 00:00:00

  • Parasite load estimation by qPCR differentiates between asymptomatic and symptomatic infection in Indian visceral leishmaniasis.

    abstract::Using quantitative PCR (qPCR), we differentiated asymptomatic and symptomatic Indian Leishmania donovani infection. qPCR on blood of 40 visceral leishmaniasis, 130 endemic, and 40 non-endemic healthy controls showed 500 times less (P < .0001) parasitemia in asymptomatic compared to the symptomatic ones and threshold o...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2014.01.031

    authors: Sudarshan M,Sundar S

    更新日期:2014-09-01 00:00:00

  • Drug-induced lymphocyte stimulation test in the prediction of drug-induced hypersensitivity to antituberculosis drugs.

    abstract::Antituberculosis (TB) chemotherapeutic drugs may cause a variety of adverse drug reactions (ADRs). To assess the potential of drug-induced lymphocyte stimulation test (DLST) in screening ADRs in patients treated with anti-TB drugs, we performed DLST in 272 TB patients (176 cases with ADRs and 96 controls without ADRs)...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2015.03.008

    authors: Sun Q,Sha W,Gui XW,Xiao YJ,Zeng WH,Sun WW,Xiao HP,Ye WY

    更新日期:2015-06-01 00:00:00

  • Prototheca zopfii isolated from a patient with olecranon bursitis.

    abstract::Prototheca zopfii was isolated from a patient with olecranon bursitis. Olecranon bursitis caused by Prototheca is a distinct clinical entity. Recognition of this infection was made by observing characteristic organisms in tissue and subsequent isolation of the organism. ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/0732-8893(87)90103-9

    authors: Naryshkin S,Frank I,Nachamkin I

    更新日期:1987-02-01 00:00:00

  • Application of a loop-mediated isothermal amplification method for the detection of pathogenic Leptospira.

    abstract::Leptospirosis is an emerging infectious disease, which is considered to be the most widespread zoonotic disease in the world. There are more than 230 known serovars in the genus Leptospira. A loop-mediated isothermal amplification (LAMP) assay for the rapid detection of pathogenic Leptospira spp. was developed and eva...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2008.10.012

    authors: Lin X,Chen Y,Lu Y,Yan J,Yan J

    更新日期:2009-03-01 00:00:00

  • Antimicrobial susceptibility and biofilm formation of Staphylococcus epidermidis small colony variants associated with prosthetic joint infection.

    abstract::We determined the frequency of isolation of non-aureus staphylococcal small colony variants (SCVs) from 31 patients with staphylococcal prosthetic joint infection (PJI) and described the antimicrobial susceptibility, auxotrophy, and biofilm-forming capacity of these SCVs. Eleven non-aureus SCVs were recovered, all of ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2012.06.029

    authors: Maduka-Ezeh AN,Greenwood-Quaintance KE,Karau MJ,Berbari EF,Osmon DR,Hanssen AD,Steckelberg JM,Patel R

    更新日期:2012-11-01 00:00:00

  • Comparative evaluation of the ARCHITECT Toxo IgG, IgM, and IgG Avidity assays for anti-Toxoplasma antibodies detection in pregnant women sera.

    abstract::We assessed the performance of the ARCHITECT Toxo IgG, IgM, and IgG Avidity assays against corresponding assays on AxSYM and Vidas using 730 sera from pregnant women. The ARCHITECT Toxo IgG and IgM assays showed a relative sensitivity of 97.5% and 89.9% and a relative specificity of 99.1% and 99.8%, respectively. If I...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2009.07.013

    authors: Gay-Andrieu F,Fricker-Hidalgo H,Sickinger E,Espern A,Brenier-Pinchart MP,Braun HB,Pelloux H

    更新日期:2009-11-01 00:00:00

  • Comparison of fluconazole minimum inhibitory concentrations in three different formulations of RPMI-1640.

    abstract::This study aimed to compare the susceptibilities of fluconazole, obtained in two laboratories, using three RPMI-1640 formulations (manufacturers') and inhibition standards (80%). A total of 39 selected Candida species (in vitro susceptible and resistant) and Cryptococcus neoformans isolates were tested in each medium ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/0732-8893(94)90097-3

    authors: Espinel-Ingroff A,Steele-Moore L

    更新日期:1994-10-01 00:00:00

  • Optimized serodiagnosis of Mycoplasma pneumoniae infections.

    abstract::Serologic methods are well established for the diagnosis of Mycoplasma pneumoniae infections in humans, but they are less sensitive than polymerase chain reaction (PCR). To improve their sensitivity, a new panel of antigens was tested. Compared with PCR results, up to 92% of PCR-positive patients were confirmed by our...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2012.02.014

    authors: Dumke R,Strubel A,Cyncynatus C,Nuyttens H,Herrmann R,Lück C,Jacobs E

    更新日期:2012-06-01 00:00:00

  • Molecular identification of Mycobacterium chimaera as a cause of infection in a patient with chronic obstructive pulmonary disease.

    abstract::This report describes a case of Mycobacterium chimaera infection in a patient with a history of chronic obstructive pulmonary disease where the organism was identified by using molecular methods. M. chimaera was identified from fresh lung tissue and from an instrument-negative mycobacterial growth indicator tube broth...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2008.12.002

    authors: Bills ND,Hinrichs SH,Aden TA,Wickert RS,Iwen PC

    更新日期:2009-03-01 00:00:00

  • Hepatitis C virus core antigen in the management of patients treated with new direct-acting antivirals.

    abstract::We evaluated the utility of Architect core antigen assay® Abbott Diagnostics (HCVAg) for monitoring patients with HCV infection and compared to HCV-RNA quantification (Cobas Ampliprep TaqMan-Roche Diagnostics). Samples from 262 patients were studied. Mean baseline HCV RNA and HCVAg levels were similar for responders (...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2017.06.006

    authors: Arboledas JCA,Guerrero IP,Rodríguez MJB,Martos ET,Pérez AB,León CC,Sierra Sánchez JF,Prieto MDL,Porcuna NC,Mochón MDO,Macías J,de la Iglesia Salgado A,Granger JR,Fernández MD,Lozano IG,Ramírez ER,Rivero A,Del Carmen Loz

    更新日期:2017-09-01 00:00:00

  • Ceftobiprole activity when tested against contemporary bacteria causing bloodstream infections in the United States (2016-2017).

    abstract::Ceftobiprole medocaril, the prodrug of ceftobiprole, is an advanced-generation cephalosporin that is approved in many European and non-European countries for the treatment of adults with hospital-acquired pneumonia (excluding ventilator-associated pneumonia) and community-acquired pneumonia and is currently being eval...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2019.01.015

    authors: Pfaller MA,Flamm RK,Duncan LR,Shortridge D,Smart JI,Hamed KA,Mendes RE,Sader HS

    更新日期:2019-07-01 00:00:00

  • Rapid diagnosis of tuberculosis by identification of Antigen 85 in mycobacterial culture system.

    abstract::The standard culture for identification of Mycobacterium tuberculosis takes a long time to perform. We introduce here a method for fast identification of M. tuberculosis in mycobacterial culture system. Antibodies to Antigen (Ag) 85 of M. tuberculosis were produced and subsequently used to develop enzyme-linked immuno...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2013.11.028

    authors: Phunpae P,Chanwong S,Tayapiwatana C,Apiratmateekul N,Makeudom A,Kasinrerk W

    更新日期:2014-03-01 00:00:00

  • Performance of the Gen-Probe amplified Mycobacterium tuberculosis direct test in a laboratory that infrequently isolates Mycobacterium tuberculosis.

    abstract::The performance of the Gen-Probe Mycobacterium tuberculosis direct (MTD) test was assessed in a laboratory whose specimens were derived from a population with a low prevalence (1.3%) of tuberculosis. A total of 339 specimens from 113 patients were included in the study. Nine of 10 MTD positive samples were culture pos...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/0732-8893(95)00065-i

    authors: Welch K,Brown G,Jonas V,Ferraro MJ

    更新日期:1995-07-01 00:00:00

  • Evaluation of a pan-serotype point-of-care rapid diagnostic assay for accurate detection of acute dengue infection.

    abstract::The catastrophic rise in dengue infections in India and globally has created a need for an accurate, validated low-cost rapid diagnostic test (RDT) for dengue. We prospectively evaluated the diagnostic performance of NS1/IgM RDT (dengue day 1) using 211 samples from a pediatric dengue cohort representing all 4 serotyp...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2016.09.020

    authors: Vivek R,Ahamed SF,Kotabagi S,Chandele A,Khanna I,Khanna N,Nayak K,Dias M,Kaja MK,Shet A

    更新日期:2017-03-01 00:00:00

  • Selection of a surrogate beta-lactam testing agent for initial susceptibility testing of doripenem, a new carbapenem.

    abstract::Doripenem, a broad-spectrum parenteral carbapenem, has potency and pharmacokinetic/pharmacodynamic features most similar to imipenem and meropenem. Because of potential delays in release of commercial testing devices post-regulatory approval (US Food and Drug Administration), "surrogate markers" offer immediate suscep...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2007.10.018

    authors: Jones RN,Sader HS,Fritsche TR,Janechek MJ

    更新日期:2007-12-01 00:00:00

  • Impact of CLIA 88 on the clinical microbiology laboratory.

    abstract::The Clinical Laboratory Improvement Amendments (CLIA 88) to the Clinical Laboratory Improvement Act of 1967 continues to undergo transformation since its implementation more than 2 years ago. The law and its subsequent regulatory modifications were intended to promote high quality in and accurate results from laborato...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/0732-8893(95)00152-2

    authors: Chapin K,Baron EJ

    更新日期:1995-09-01 00:00:00

  • Comparison of eight commercial enzyme immunoassays for the detection of Clostridium difficile from stool samples and effect of strain type.

    abstract::We compared the performance of 8 Clostridium difficile enzyme immunoassays to cell cytotoxicity neutralization assay and toxigenic culture. The effect of strain type on assay performance was also examined. There were a total of 71 (14.4%) samples in which C. difficile was recovered; 58 (81.7%) of 71 were toxigenic. Co...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2012.01.005

    authors: René P,Frenette CP,Schiller I,Dendukuri N,Brassard P,Fenn S,Loo VG

    更新日期:2012-05-01 00:00:00

  • Eggerthella hongkongensis sp. nov. and eggerthella sinensis sp. nov., two novel Eggerthella species, account for half of the cases of Eggerthella bacteremia.

    abstract::Eggerthella, one of the human gut flora, was rarely reported to cause bacteremia in the literature. We describe the application of 16S ribosomal RNA gene sequencing in defining the epidemiology and clinical significance of Eggerthella bacteremia during a 4-year period. Among 55 clinically significant blood culture iso...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2004.04.012

    authors: Lau SK,Woo PC,Woo GK,Fung AM,Wong MK,Chan KM,Tam DM,Yuen KY

    更新日期:2004-08-01 00:00:00

  • Identification by 16S ribosomal RNA gene sequencing of an Enterobacteriaceae species with ambiguous biochemical profile from a renal transplant recipient.

    abstract::Traditional ways of identification of bacteria by phenotypic characteristics cannot be used for non-cultivable organisms and organisms with unusual biochemical profiles. In this study, an Enterobacteriaceae was isolated in pure growth from the mid-stream urine of a 67-year old renal transplant recipient with urinary t...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/s0732-8893(01)00206-1

    authors: Woo PC,Cheung EY,Leung K,Yuen K

    更新日期:2001-02-01 00:00:00

  • Detection of rubella immunoglobulin M from dried venous blood spots using a commercial enzyme immunoassay.

    abstract::Confirmation of the diagnosis is critical to disease control in measles elimination and rubella control programs. In countries with limited infrastructure and laboratory capacity, collection, transport, and testing of venous blood samples may be difficult. We report the adaptation of a commercial enzyme immunoassay fo...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2005.06.017

    authors: Karapanagiotidis T,Riddell M,Kelly H

    更新日期:2005-10-01 00:00:00

  • Changes in Haemophilus influenzae capsule locus: possible emergence of novel variants in Brazil.

    abstract::A total of 28 strains of Haemophilus influenzae (Hi) a and b isolated from clinical samples before and after the introduction of the Hib conjugate vaccine in Brazil were analyzed to determine variants of the capsular gene. Our results suggest the occurrence of new variants closely related to types I and II previously ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2010.05.011

    authors: Batalha A,de Almeida AE,Caldeira NG,de Filippis I

    更新日期:2010-10-01 00:00:00

  • Evaluation of a SARS-CoV-2 lateral flow assay using the plaque reduction neutralization test.

    abstract::As new tests and technologies advance our understanding and diagnostic capabilities of the severe acute respiratory syndrome coronavirus 2 and the coronavirus disease 2019, they must be appropriately validated to make sure test performance is following manufacturer claims. In this study, we evaluated the Vazyme 2019-n...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2020.115248

    authors: Xie X,Nielsen MC,Muruato AE,Fontes-Garfias CR,Ren P

    更新日期:2021-02-01 00:00:00

  • Diagnostic performance of a new automated heterophile antibody test in adults and children.

    abstract::Epstein-Barr virus serologic profiles were obtained from 618 patients using an automated platform, including the new BioPlex heterophile assay. When compared with 2 IgM viral capsid antigen (VCA) assays and a predicate heterophile assay, the BioPlex heterophile assay was more likely to be associated with serologic pat...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2008.02.016

    authors: Klutts JS,Wu AH,Smith A,Yen-Lieberman B,Gronowski AM

    更新日期:2008-07-01 00:00:00

  • Activity of ceftazidime-avibactam against Escherichia coli isolates from U.S. veterans (2011) in relation to co-resistance and sequence type 131 (ST131) H30 and H30Rx status.

    abstract::Escherichia coli ST131, with its multidrug-resistance-associated H30R1 and H30Rx clonal subsets within the H30R subclone, causes most antimicrobial-resistant E. coli infections. The activity of ceftazidime-avibactam (CZA) against ST131 strains is undefined. We determined CZA MICs for 595 E. coli clinical isolates from...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2020.115034

    authors: Johnston BD,Thuras PD,Johnson JR

    更新日期:2020-07-01 00:00:00

  • Comparison of BD Phoenix and bioMérieux Vitek 2 automated systems for the detection of macrolide-lincosamide-streptogramin B resistance among clinical isolates of Staphylococcus.

    abstract::Two automated systems, BD Phoenix and bioMérieux Vitek 2, were compared to a double-disk diffusion method for the detection of inducible resistance to clindamycin in Staphylococcus spp. Analysis of 524 clinical isolates revealed sensitivity and specificity of 100% and 99.6%, respectively, for Phoenix and 91.1% and 99....

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2011.12.003

    authors: Buchan BW,Anderson NW,Ledeboer NA

    更新日期:2012-03-01 00:00:00