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Comparison of the performance of IFA, CFA, and ELISA assays for the serodiagnosis of acute Q fever by quality assessment.

Abstract：

:The indirect immunofluorescence assay (IFA) is considered the reference method for diagnosing Q fever, but serology is also performed by complement fixation assay (CFA) or enzyme-linked immunosorbent assay (ELISA). However, comparability between these assays is not clear, and therefore a quality assessment was performed. A total of 25 serum samples from negative controls, Q fever patients, and a serial diluted high-positive sample were analyzed in 10 Dutch laboratories. Six laboratories performed CFA, 5 performed IFA, and 5 performed ELISAs. Three international reference laboratories from Australia, France, and the USA also participated in this study. Qualitative values between laboratories using the same methods were within close range, and all 3 methods correctly identified acute Q fever patients. The IFA, ELISA, and CFA are all suitable serodiagnostic assays to diagnose acute Q fever, but the IFA remains an important tool in the follow-up of patients and in identifying patients at risk for developing chronic Q fever.

journal_name

Diagn Microbiol Infect Dis

journal_title

Diagnostic microbiology and infectious disease

authors

Herremans T,Hogema BM,Nabuurs M,Peeters M,Wegdam-Blans M,Schneeberger P,Nijhuis C,Notermans DW,Galama J,Horrevorts A,van Loo IH,Vlaminckx B,Zaaijer HL,Koopmans MP,Berkhout H,Socolovschi C,Raoult D,Stenos J,Nicholson W

doi

10.1016/j.diagmicrobio.2012.09.001

subject

Has Abstract

pub_date

2013-01-01 00:00:00

pages

16-21

issue

1

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(12)00356-2

journal_volume

75

pub_type

杂志文章

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