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Loop-mediated isothermal amplification (LAMP) for the rapid detection of Mycoplasma genitalium.

Abstract：

:Mycoplasma genitalium is a sexually transmissible, pathogenic bacterium and a significant cause of nongonococcal urethritis in both men and women. Due to the difficulty of the culture of M. genitalium from clinical samples, the laboratory diagnosis of M. genitalium infection is almost exclusively carried out using nucleic acid amplification tests. Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification technology, utilising a set of 4 primers specific to 6 distinct regions of the target DNA sequence, in order to amplify target DNA in a highly specific and rapid manner. A LAMP assay was designed to the pdhD gene of M. genitalium, and the limit of detection of the assay was determined as 10 fg of M. genitalium genomic DNA, equating to ~16 copies of the M. genitalium genome, which was equally sensitive as a gold standard 16S rRNA polymerase chain reaction assay.

journal_name

Diagn Microbiol Infect Dis

journal_title

Diagnostic microbiology and infectious disease

authors

Edwards T,Burke P,Smalley HB,Gillies L,Longhurst D,Vipond B,Hobbs G

doi

10.1016/j.diagmicrobio.2015.05.010

subject

Has Abstract

pub_date

2015-09-01 00:00:00

pages

13-7

issue

1

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(15)00190-X

journal_volume

83

pub_type

杂志文章

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