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Detection of stx1, stx2, and eae genes of enterohemorrhagic Escherichia coli using SYBR Green in a real-time polymerase chain reaction.

Abstract：

:We report a multiplex real-time polymerase chain reaction method for detecting enterohemorrhagic Escherichia coli (EHEC) from strains or stool specimens. This assay detected the virulence genes stx1, stx2, and eae, without the use of probes. The method, which was validated on a collection of 143 EHEC strains, is simple, rapid, cost-effective, and sensitive.

journal_name

Diagn Microbiol Infect Dis

journal_title

Diagnostic microbiology and infectious disease

authors

Chassagne L,Pradel N,Robin F,Livrelli V,Bonnet R,Delmas J

doi

10.1016/j.diagmicrobio.2009.01.031

subject

Has Abstract

pub_date

2009-05-01 00:00:00

pages

98-101

issue

1

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(09)00052-2

journal_volume

64

pub_type

杂志文章

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