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Evaluation and comparison of automated and manual ELISA for diagnosis of chronic pulmonary aspergillosis (CPA) in Indonesia.

Abstract：

:Pulmonary tuberculosis (TB) is one of the common risk factors for chronic pulmonary aspergillosis (CPA). A positive Aspergillus IgG is a key element of the diagnosis of CPA but this has not been studied in Indonesia. We conducted studies with patients at the end of TB therapy in Indonesia. We performed receiver operating curve (ROC) analysis to determine the optimum cutoff of the Aspergillus-specific IgG level (Immulite and Dynamiker ELISA) in those patients who met criteria of CPA in relation to control groups. In 203 TB patients, 26 (13%) patients had clinical and radiological features of CPA. We derived optimum cutoffs for Immulite Aspergillus-specific IgG of 11.5 mg/L and Dynamiker anti-galactomannan IgG of 106.8 AU/mL (sensitivity 89% and 83%, specificity 78% and 51%, respectively). The currently accepted Aspergillus-specific IgG cutoff of Immulite and Dynamiker assays for CPA diagnosis may require slight adjustment for the Indonesian population.

journal_name

Diagn Microbiol Infect Dis

journal_title

Diagnostic microbiology and infectious disease

authors

Setianingrum F,Rozaliyani A,Syam R,Adawiyah R,Tugiran M,Sari CYI,Burhan E,Wahyuningsih R,Rautemaa-Richardson R,Denning DW

doi

10.1016/j.diagmicrobio.2020.115124

subject

Has Abstract

pub_date

2020-11-01 00:00:00

pages

115124

issue

3

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(20)30501-0

journal_volume

98

pub_type

杂志文章

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