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Multicenter evaluation of the Verigene Gram-negative blood culture nucleic acid test for rapid detection of bacteria and resistance determinants in positive blood cultures.

Abstract：

:The Verigene Gram-Negative Blood Culture Nucleic Acid Test (BC-GN) is a microarray-based assay that enables rapid detection of 9 common Gram-negative bacteria and 6 resistance determinants directly from positive blood cultures. We compared the performance of BC-GN with currently used automated systems, testing 141 clinical blood cultures and 205 spiked blood cultures. For identification of BC-GN target organisms in clinical and spiked blood cultures, the BC-GN assay showed 98.5% (130/132) and 98.9% (182/184) concordance, respectively. Of 140 resistance genes positively detected in clinical and spiked blood cultures with the BC-GN test, 139 (99.3%) were confirmed by PCR, and the detection results were consistent with the resistance phenotypes observed. The BC-GN assay, thus, can potentially improve care for sepsis patients by enabling timely detection and targeted antimicrobial therapy.

journal_name

Diagn Microbiol Infect Dis

journal_title

Diagnostic microbiology and infectious disease

authors

Uno N,Suzuki H,Yamakawa H,Yamada M,Yaguchi Y,Notake S,Tamai K,Yanagisawa H,Misawa S,Yanagihara K

doi

10.1016/j.diagmicrobio.2015.08.004

subject

Has Abstract

pub_date

2015-12-01 00:00:00

pages

344-8

issue

4

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(15)00293-X

journal_volume

83

pub_type

杂志文章,多中心研究

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