当前位置： SCI文献检索 > DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE期刊下所有文献 > Application of recombinant fimbrial protein for the specific detection of Salmonella enteritidis infection in poultry.

Application of recombinant fimbrial protein for the specific detection of Salmonella enteritidis infection in poultry.

Abstract：

:A number of disease outbreaks of Salmonella enterica serotype enteritidis (SE) in humans have been traced to the consumption of SE-contaminated egg and egg products. A rapid, specific, and inexpensive method of detecting SE infection in poultry is necessary to reduce human outbreaks. We evaluated rSEF14 fimbrial antigen of SE for specific detection of SE-infected birds in latex agglutination test and enzyme-linked immunosorbent assay. rSEF14 antigen was highly specific in identifying birds infected with SE. The sera from birds infected with closely related serogroup-D Salmonella and other avian pathogens did not react with rSEF14 antigen. The rSEF14 antigen identified antibodies in serum of 88% of birds during the first 2 weeks of infection, and 100% of the birds subsequently. The SE-specific antibodies were detected in egg yolk as early as 6 days post-infection in rSEF14-enzyme-linked immunosorbent assay. Our results suggest that rSEF14-based assays could be used as screening tests for detection of SE antibodies and would overcome the cross reactions observed with existing serological tests.

journal_name

Diagn Microbiol Infect Dis

journal_title

Diagnostic microbiology and infectious disease

authors

Rajashekara G,Munir S,Lamichhane CM,Back A,Kapur V,Halvorson DA,Nagaraja KV

doi

10.1016/s0732-8893(98)00091-1

subject

Has Abstract

pub_date

1998-11-01 00:00:00

pages

147-57

issue

3

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(98)00091-1

journal_volume

32

pub_type

杂志文章

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