Cephalothin is not a reliable surrogate marker for oral cephalosporins in susceptibility testing of Enterobacteriaceae causing urinary tract infection.

Abstract:

:Vitek® 2 (bioMérieux) is a widely used commercial antimicrobial susceptibility test (AST) system. AST-N244 card includes cephalothin as first-generation cephalosporin. We compared the cephalothin susceptibility results obtained with Vitek® 2 AST-N244 to those obtained by broth microdilution (BMD) and disk diffusion (DD) for 212 urinary Enterobacteriaceae. We also evaluated the differences between cefazolin and cephalothin susceptibility results. The overall performance of Vitek® 2 for cephalothin testing was 74.5% and 76.4% category agreement compared to BMD and DD, respectively; 84.4% essential agreement; very major errors 15.2% and 11.1% compared to BMD and DD; major errors 0% compared to both methods; and minor errors 22.2% and 21.7% compared to BMD and DD. Regarding correlation between cephalothin and cefazolin, the differences observed were statistically significant (P<0.0001) for the 167 Escherichia coli included (39.5% cephalothin susceptible versus 92.2% cefazolin susceptible by BMD; 41.9% cephalothin susceptible versus 93.4% cefazolin susceptible by DD). Vitek® 2 should provide cefazolin instead of cephalothin as a surrogate marker for oral cephalosporins on the urinary AST-244 cards in order to follow the CLSI (2016) recommendations.

authors

López IA,Montes JC,Álvarez MJ,Mazarrasa CF,Martínez-Martínez L

doi

10.1016/j.diagmicrobio.2016.08.017

subject

Has Abstract

pub_date

2016-12-01 00:00:00

pages

412-416

issue

4

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(16)30256-5

journal_volume

86

pub_type

杂志文章
  • In vitro activity of recombinant lysostaphin-antibiotic combinations toward methicillin-resistant Staphylococcus aureus.

    abstract::In vitro bactericidal experiments were performed with combinations of recombinant lysostaphin and various antibiotics against five strains of Staphylococcus aureus, including clinical isolates of methicillin-resistant S. aureus (MRSA). Sublethal concentrations of lysostaphin combined with beta-lactam antibiotics (incl...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/0732-8893(93)90034-5

    authors: Polak J,Della Latta P,Blackburn P

    更新日期:1993-11-01 00:00:00

  • Real-world performance of a microarray-based rapid diagnostic for Gram-positive bloodstream infections and potential utility for antimicrobial stewardship.

    abstract::The Verigene Gram-positive blood culture assay (BC-GP) is a microarray-based rapid diagnostic test, which includes targets for 12 bacterial species and 3 resistance determinants. We prospectively compared the diagnostic accuracy of the BC-GP to routine microbiologic methods and evaluated the potential of the BC-GP for...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2014.09.025

    authors: Aitken SL,Hemmige VS,Koo HL,Vuong NN,Lasco TM,Garey KW

    更新日期:2015-01-01 00:00:00

  • Direct detection of methicillin-resistant Staphylococcus aureus from blood cultures using an immunochromatographic immunoassay-based MRSA rapid kit for the detection of penicillin-binding protein 2a.

    abstract::Using an EZ-Step MRSA rapid kit, a novel screening test for methicillin-resistant Staphylococcus aureus (MRSA) that detects penicillin-binding protein 2a, 34 of 36 MRSA-positive clinical blood culture samples were positive on direct testing (sensitivity, 94.4%), whereas 21 of 21 methicillin-susceptible S. aureus-posit...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2010.02.018

    authors: Shin KS,Song HG,Kim H,Yoon S,Hong SB,Koo SH,Kim J,Kim J,Roh KH

    更新日期:2010-07-01 00:00:00

  • Successful use of culture and enrichment for the detection of OXA-181-producing Escherichia coli from rectal swab samples falsely categorized as negative by Xpert® Carba-R.

    abstract::An OXA-181 producing Escherichia coli isolate that went recurrently undetected directly from rectal swab sample using Xpert® Carba-R, was successfully detected using ertapenem supplemented broth enrichment followed by culture-based method. Our data suggest that implementation of culture-based methods plus enrichment m...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2019.114909

    authors: Girlich D,Ouzani S,Langlois I,Begasse C,Arangia N,Fortineau N,Naas T,Dortet L

    更新日期:2020-01-01 00:00:00

  • Single gene target bacterial identification. groEL gene sequencing for discriminating clinical isolates of Burkholderia pseudomallei and Burkholderia thailandensis.

    abstract::Proper identification of Burkholderia pseudomallei and Burkholderia thailandensis is crucial in guiding clinical management of patients with suspected melioidosis, as more than 99% of cases of melioidosis are caused by B. pseudomallei, whereas B. thailandensis is only responsible for causing less than 1% of the cases....

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/s0732-8893(02)00439-x

    authors: Woo PC,Woo GK,Lau SK,Wong SS,Yuen Ky

    更新日期:2002-10-01 00:00:00

  • Low-level resistance to glycopeptides amongst staphylococcus species: surveillance in a university hospital and evaluation of a vancomycin screening agar.

    abstract::The prevalence of low-level resistance to glycopeptides (teicoplanin MIC > or = 8 microg/mL and vancomycin MIC > or = 4 microg/mL) among staphylococci was investigated over a 15 month period. A total of 2,279 isolates (1,519 S. aureus, 760 coagulase-negative staphylococcus (CNS)) were screened using inoculum of 10(6) ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/s0732-8893(01)00302-9

    authors: Park YJ,Jun Park J,Lee SO,Oh EJ,Kee Kim B

    更新日期:2001-11-01 00:00:00

  • Enterobacterial repetitive intergenic consensus PCR is a useful tool for typing Mycobacterium chelonae and Mycobacterium abscessus isolates.

    abstract::Outbreaks of rapidly growing mycobacterium (RGM) infections are increasingly being reported worldwide. Information about genetic relatedness of isolates obtained during outbreaks can provide opportunities for prompt intervention. Pulsed-field gel electrophoresis (PFGE) is expensive, time consuming, and labor intensive...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2006.01.006

    authors: Sampaio JL,Viana-Niero C,de Freitas D,Höfling-Lima AL,Leão SC

    更新日期:2006-06-01 00:00:00

  • Kinetics of CMV seroconversion in a Swiss pregnant women population.

    abstract::Retrospective evaluation of the kinetics of cytomegalovirus (CMV) seroconversion with CMV IgM, IgG, and IgG avidity assays, in a Swiss pregnant women population, has shown that the current published CMV serologic diagnostic algorithms were valid and fit for use. In 19% of the cases analyzed, CMV-specific IgM was detec...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2012.03.016

    authors: Maine GT,Stricker R,Stricker R

    更新日期:2012-07-01 00:00:00

  • The outcome of candiduria in pediatric patients.

    abstract::The presence of Candida in the urine of a seriously ill, pediatric patient presents a management problem because of a lack of information concerning the natural history of candiduria and its relationship to disseminated candidiasis. In this retrospective study, the outcome of candiduria was examined in a group of 54 p...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/0732-8893(91)90045-h

    authors: Chun CS,Turner RB

    更新日期:1991-03-01 00:00:00

  • A quick and cost-effective method for diagnosing disseminated histoplasmosis in children.

    abstract::The examination of fecal mucus for detecting yeast cells of Histoplasma capsulatum has proved to be a useful tool for diagnosing disseminated histoplasmosis in paediatric patients in a study of 13 cases carried out in Ecuador. ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2006.10.016

    authors: Vega W,Almeida R,Miño G,Gené J,Guarro J

    更新日期:2007-04-01 00:00:00

  • Detergent extract from chlamydial elementary bodies used as antigen for enzyme-linked immunosorbent assay.

    abstract::Soluble antigen (SA) from chlamydial elementary bodies (EBs) was extracted with N-lauroylsarcosine. The extracted SA composed of lipopolysaccharide (LPS) and proteins was compared with EBs using an enzyme-linked immunosorbent assay (ELISA). Patient sera from natural chlamydial infections exhibited ELISA mean absorbanc...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/s0732-8893(02)00362-0

    authors: Quevedo Diaz MA,Melnicáková J,Kazár J,Hoch J,Senfleben S,Lukácová M

    更新日期:2002-04-01 00:00:00

  • Impact of a pharmacist-driven care package on Staphylococcus aureus bacteremia management in a large community healthcare network: A propensity score-matched, quasi-experimental study.

    abstract:OBJECTIVES:Staphylococcus aureus bacteremia (SAB) is an important cause of morbidity and mortality. Suboptimal treatment has been associated with poor patient outcomes. Our antimicrobial stewardship program (ASP) evaluated SAB management based on predefined performance measures both prior to and after instituting a "ca...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2017.10.001

    authors: Smith JR,Frens JJ,Snider CB,Claeys KC

    更新日期:2018-01-01 00:00:00

  • A novel OXA-10-like beta-lactamase is present in different Enterobacteriaceae.

    abstract::OXA 101, a novel OXA-10 like enzyme, was found forming part of a class 1 integron located in a conjugative plasmid in three different species of Enterobacteriaceae. This beta-lactamase is related to OXA-35 and OXA-56 and displays a narrow substrate hydrolysis profile. ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2009.09.010

    authors: Porto A,Ayala J,Gutkind G,Di Conza J

    更新日期:2010-02-01 00:00:00

  • Detection of Pneumocystis carinii by DNA amplification in human immunodeficiency virus-positive patients.

    abstract::The opportunistic pathogen Pneumocystis carinii (PC) is a frequent cause of a life-threatening pneumonia in human immunodeficiency virus (HIV)-infected individuals and in other immunocompromised hosts. Specimens obtained from 128 bronchoalveolar lavage (BAL) fluid samples from 123 HIV-positive patients with pulmonary ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 临床试验,杂志文章

    doi:10.1016/s0732-8893(97)00126-0

    authors: Savoia D,Millesimo M,Cassetta I,Forno B,Caramello P

    更新日期:1997-10-01 00:00:00

  • Optimized serodiagnosis of Mycoplasma pneumoniae infections.

    abstract::Serologic methods are well established for the diagnosis of Mycoplasma pneumoniae infections in humans, but they are less sensitive than polymerase chain reaction (PCR). To improve their sensitivity, a new panel of antigens was tested. Compared with PCR results, up to 92% of PCR-positive patients were confirmed by our...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2012.02.014

    authors: Dumke R,Strubel A,Cyncynatus C,Nuyttens H,Herrmann R,Lück C,Jacobs E

    更新日期:2012-06-01 00:00:00

  • In vitro activity and killing effect of the synthetic hybrid cecropin A-melittin peptide CA(1-7)M(2-9)NH(2) on methicillin-resistant nosocomial isolates of Staphylococcus aureus and interactions with clinically used antibiotics.

    abstract::The in vitro activity of CA(1-7)M(2-9)NH(2), a 15-residue synthetic hybrid peptide derived from the sequences of cecropin A and melittin, alone and in combination with amoxicillin-clavulanate, imipenem, clarithromycin, ciprofloxacin, rifampin, and vancomycin, was investigated against 40 nosocomial isolates of methicil...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2004.02.008

    authors: Giacometti A,Cirioni O,Kamysz W,D'Amato G,Silvestri C,Simona Del Prete M,Lukasiak J,Scalise G

    更新日期:2004-07-01 00:00:00

  • Characterization of Streptococcus pyogenes isolates responsible for adult meningitis in France from 2003 to 2013.

    abstract::Sixty-three cases of Streptococcus pyogenes meningitis in adults were studied. Three predominant emm types were associated with meningitis: emm1 (44%), emm3 (11%), and emm6 (11%). Streptococcal toxic shock syndrome and mortality rates were 40% and 38%, respectively. ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2015.12.006

    authors: Plainvert C,Doloy A,Joubrel C,Maataoui N,Dmytruk N,Touak G,Collobert G,Fouet A,Poyart C,Loubinoux J

    更新日期:2016-04-01 00:00:00

  • Cytomegalovirus antibody detection in blood donors and mothers of very low birth weight neonates by using three serologic methods.

    abstract::We compared three serologic methods for cytomegalovirus (CMV) antibody detection and determined the CMV antibody seroprevalence of blood donors and mothers of very low birth weight (less than 1250 g) neonates in the Greater Hartford region. CMV serology was determined for 577 healthy blood donors as well as for 147 mo...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/0732-8893(92)90035-r

    authors: Eisenfeld L,McLaughlin JC,Mayo D,Klevjer-Anderson P,Silver H,Krause P,Anderson J,Herson V,Savidakis J,Lazar AM

    更新日期:1992-02-01 00:00:00

  • Rapid Chagas diagnosis in clinical settings using a multiparametric assay.

    abstract::The development of an immunoassay for detecting circulating antibodies against Trypanosoma cruzi with a good performance appears to be crucial in clinical settings for the management of Chagas disease. Here we propose a new automated ELISA test performed on serum samples using 2 different T. cruzi antigens (a recombin...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2012.12.005

    authors: Pierimarchi P,Cerni L,Alarcón de Noya B,Nicotera G,Díaz-Bello Z,Angheben A,Scacciatelli D,Zonfrillo M,Recinelli G,Serafino A

    更新日期:2013-04-01 00:00:00

  • Partial comparison of the NxTAG Respiratory Pathogen Panel Assay with the Luminex xTAG Respiratory Panel Fast Assay V2 and singleplex real-time polymerase chain reaction for detection of respiratory pathogens.

    abstract::In this study, 185 nasopharyngeal swabs were tested to compare the sensitivity and specificity of the Luminex NxTAG (NxTAG) Respiratory Pathogen Panel (RPP) Assay with those of the Luminex Respiratory Virus Panel (RVP) Fast Assay v2 and singleplex real-time polymerase chain reaction (PCR). The NxTAG Assay identified a...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2016.06.018

    authors: Esposito S,Scala A,Bianchini S,Presicce ML,Mori A,Sciarrabba CS,Fior G,Principi N

    更新日期:2016-09-01 00:00:00

  • Development of a multiplex TaqMan real-time PCR assay for the detection of Chlamydia psittaci and Chlamydia pneumoniae in human clinical specimens.

    abstract::Diagnosis of Chlamydia psittaci and Chlamydia pneumoniae infections has traditionally relied on serological assays. We developed a multiplex real-time PCR assay for detection of C. psittaci, C. pneumoniae and an internal control. Results of this assay demonstrated 100% concordance compared to results of previously tes...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2017.11.014

    authors: Wolff BJ,Morrison SS,Winchell JM

    更新日期:2018-03-01 00:00:00

  • Impact of inter-genotypic recombination and probe cross-reactivity on the performance of the Abbott RealTime HCV Genotype II assay for hepatitis C genotyping.

    abstract::The Abbott RealTime HCV Genotype II assay (Abbott-RT-HCV assay) is a real-time PCR based genotyping method for hepatitis C virus (HCV). This study measured the impact of inter-genotypic recombination and probe cross-reactivity on the performance of the Abbott-RT-HCV assay. 517 samples were genotyped using the Abbott-R...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2017.12.024

    authors: Sridhar S,Yip CCY,Chan JFW,To KKW,Cheng VCC,Yuen KY

    更新日期:2018-05-01 00:00:00

  • Five-year analysis of Haemophilus influenzae isolates with reduced susceptibility to fluoroquinolones: prevalence results from the SENTRY antimicrobial surveillance program.

    abstract::The appearance of resistance or reduced susceptibility to fluoroquinolones among Hemophilus influenzae has been documented for nearly a decade. Over this time, the use of fluoroquinolones for the treatment of respiratory infections including commonly isolated bacterial causes of community-acquired infections has marke...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/s0732-8893(03)00016-6

    authors: Biedenbach DJ,Jones RN

    更新日期:2003-05-01 00:00:00

  • Comparative evaluation of etest for susceptibility testing Neisseria meningitidis with eight antimicrobial agents. An investigation using U.S. Food and Drug Administration regulatory criteria.

    abstract::Invasive infections caused by Neisseria meningitidis continue to be a serious clinical problem for therapy, epidemiology, and potential prophylaxis. Multiple antimicrobial resistances have emerged among meningococcal strains including elevated MICs to penicillin, sulfonamides, tetracyclines, and rifampin. Thus, the ne...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/s0732-8893(96)00223-4

    authors: Marshall SA,Rhomberg PR,Jones RN

    更新日期:1997-03-01 00:00:00

  • Effect of treatment of shell vial cell cultures with dimethyl sulfoxide and dexamethasone and age of MRC-5 monolayers for detection of cytomegalovirus.

    abstract::Pretreatment of MRC-5 cell monolayers in commercially prepared shell vials with 1% dimethyl sulfoxide (DMSO) and 10(-5) mol/L dexamethasone (DEX) was evaluated. Preliminary experiments indicated enhanced infectivity of AD-169 for pretreated MRC-5 cells in shell vials of ages 9 and 16 days. Compared with untreated shel...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/0732-8893(90)90052-w

    authors: Fedorko DP,Ilstrup DM,Smith TF

    更新日期:1990-01-01 00:00:00

  • Evaluation of antimicrobial susceptibility for beta-lactams using the Etest method against clinical isolates from 100 medical centers in Japan (2006).

    abstract::This antimicrobial resistance surveillance study was performed in 100 medical centers. Susceptibility testing (Etest; AB BIODISK, Solna, Sweden) of 9152 strains including Escherichia coli (991 strains), Klebsiella spp. (1000 strains), Enterobacter spp. (971 strains), Citrobacter spp. (803 strains), indole-positive Pro...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2007.08.006

    authors: Ishii Y,Tateda K,Yamaguchi K,Japan Antimicrobial Resistance Surveillance Participants Group (JARS).

    更新日期:2008-02-01 00:00:00

  • Evaluation of rapid culture centrifugation method for adenovirus detection in stools.

    abstract::Routine laboratory testing for adenovirus (Adv) requires a procedure that is rapid and reliable, especially for samples from children and immunosuppressed patients, when diarrhea may signal the onset of severe gastrointestinal disorders. An improved culture technique for Adv isolation, using centrifugation step of 24-...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/0732-8893(95)00271-5

    authors: Durepaire N,Ranger-Rogez S,Denis F

    更新日期:1996-01-01 00:00:00

  • Comparison of a conventional and nested PCR for diagnostic confirmation and genotyping of Orientia tsutsugamushi.

    abstract::A nested polymerase chain reaction (PCR) targeting the 56-kDa antigen gene is currently the most commonly used molecular technique for confirmation of scrub typhus and genotyping of Orientia tsutsugamushi. In this study, we have compared the commonly used nested PCR (N-PCR) with a single-step conventional PCR (C-PCR) ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2013.11.030

    authors: Janardhanan J,Prakash JA,Abraham OC,Varghese GM

    更新日期:2014-05-01 00:00:00

  • Rapid identification of medically important Candida to species level by polymerase chain reaction and single-strand conformational polymorphism.

    abstract::Invasive fungal disease has taken a great toll on immunocompromised patients. With the emergence of fluconazole and amphotericin B resistance, the rapid identification of fungi to species level is of clinical relevance in guiding appropriate antifungal therapy. Among these opportunistic fungi, Candida species are the ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/s0732-8893(00)00171-1

    authors: Hui M,Ip M,Chan PK,Chin ML,Cheng AF

    更新日期:2000-10-01 00:00:00

  • Molecular epidemiology of KPC-2-producing Enterobacteriaceae (non-Klebsiella pneumoniae) isolated from Brazil.

    abstract::In Brazil, since 2009, there has been an ever increasing widespread of the bla(KPC-2) gene, mainly in Klebsiella pneumoniae. This study aims to assess the molecular epidemiology and genetic background of this gene in Enterobacteriaceae (non-K. pneumoniae) species from 9 Brazilian states between 2009 and 2011. Three hu...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2015.04.002

    authors: Tavares CP,Pereira PS,Marques Ede A,Faria C Jr,de Souza Mda P,de Almeida R,Alves Cde F,Asensi MD,Carvalho-Assef AP

    更新日期:2015-08-01 00:00:00