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Comparison of FTD® respiratory pathogens 33 and a singleplex CDC assay for the detection of respiratory viruses: A study from Cameroon.

Abstract：

INTRODUCTION:This study compares the detection of 14 common respiratory viruses by two different real-time reverse transcription polymerase chain reaction (rRT-PCR) methods: in house singleplex tests developed by the Centers for Disease Control and Prevention and the commercially available Fast Track Diagnostic (FTD®) Respiratory Pathogens 33 multiplex test. METHODS:A total of 217 nasopharyngeal swabs were analyzed using CDC singleplex rRT-PCR and FTD® Respiratory Pathogens 33 multiplex assays, for the detection of 14 respiratory viruses. RESULTS:The results showed that 179/217 (82.5%) samples were positive with the singleplex method and 183/217 (84.3%) with the FTD® Respiratory Pathogens 33 multiplex test. Excellent or satisfactory agreement was obtained for all viruses (k > 0.6) except Parainfluenzavirus 4 (k = 0.24) and influenza B (k = 0.51). CONCLUSION:Although the multiplex FTD kits were more expensive than the singleplex assay, the FTD kits yielded rapid results in a shorter timeframe, increasing efficiency of diagnosis.

journal_name

Diagn Microbiol Infect Dis

journal_title

Diagnostic microbiology and infectious disease

authors

Kenmoe S,Tcharnenwa C,Monamele GC,Kengne CN,Ripa MN,Whitaker B,Alroy KA,Balajee SA,Njouom R

doi

10.1016/j.diagmicrobio.2019.01.007

subject

Has Abstract

pub_date

2019-07-01 00:00:00

pages

236-242

issue

3

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(18)30448-6

journal_volume

94

pub_type

杂志文章

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