Direct detection of Streptococcus pneumoniae in positive blood cultures by real-time polymerase chain reaction.

Abstract:

:We developed a real-time polymerase chain reaction specific for Streptococcus pneumoniae to be applied directly from blood culture bottles without previous DNA extraction step. For the 128 blood culture bottles tested, the assay had 94% and 98.4% sensitivity and specificity, respectively. This assay provides rapid and accurate identification of this pathogen.

authors

Selva L,Esteva C,Gené A,de Sevilla MF,Hernandez-Bou S,Muñoz-Almagro C

doi

10.1016/j.diagmicrobio.2009.05.023

subject

Has Abstract

pub_date

2010-02-01 00:00:00

pages

204-6

issue

2

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(09)00235-1

journal_volume

66

pub_type

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