当前位置： SCI文献检索 > DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE期刊下所有文献 > An integrated microfluidic system using magnetic beads for virus detection.

An integrated microfluidic system using magnetic beads for virus detection.

Abstract：

:An integrated system capable of sample pretreatment using antibody-conjugated magnetic beads and one-step reverse transcriptase-polymerase chain reaction (RT-PCR) on a microfluidic system was developed to accelerate the detection of RNA viruses such as dengue virus or enterovirus 71. The targeted virus in the sample was first captured by the specific antibody-conjugated magnetic beads, which were manipulated by micro-electromagnets made of micro-electro-mechanical systems technology. The RNA of the targeted virus then underwent thermal lysis and was reverse-transcripted to cDNA using a microRT-PCR module. The sensitivity to detect dengue virus is around 10-100 PFU, which is equivalent to the commercial RNA extraction kit and a large-scale RT-PCR machine. This microsystem can specifically detect 4 serotypes of dengue virus, as well as enterovirus 71. The specificity was warranted by both antibody and primer. The microfluidic system allows automatic process of sample including mixing, incubation, and reaction. The antibody-conjugated magnetic beads offer sample pretreatment of purification and concentration. The integration of antibody-conjugated magnetic beads into the microfluidic system is promising for fast molecular diagnosis of microorganisms.

journal_name

Diagn Microbiol Infect Dis

journal_title

Diagnostic microbiology and infectious disease

authors

Lee WC,Lien KY,Lee GB,Lei HY

doi

10.1016/j.diagmicrobio.2007.07.010

subject

Has Abstract

pub_date

2008-01-01 00:00:00

pages

51-8

issue

1

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(07)00331-8

journal_volume

60

pub_type

杂志文章

相关文献

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE文献大全
  • Comparison of 9 different PCR primers for the rapid detection of severe acute respiratory syndrome coronavirus using 2 RNA extraction methods.

    abstract：:The sensitivity and specificity of various severe acute respiratory syndrome coronavirus (SARS-CoV) PCR primer and probe sets were evaluated through the use of commercial kits and in-house amplification formats. Conventional and real-time PCR assays were performed using a heat-block thermocycler ABI 9600, the Roche Li...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2005.03.007

    authors： Chui L,Drebot M,Andonov A,Petrich A,Glushek M,Mahony J

    更新日期：2005-09-01 00:00:00

  • Assessing next-generation sequencing and 4 bioinformatics tools for detection of Enterovirus D68 and other respiratory viruses in clinical samples.

    abstract：:We used 4 different bioinformatics algorithms to evaluate the application of a metagenomic shot-gun sequencing method in detection of Enterovirus D68 and other respiratory viruses in clinical specimens. Our data supported that next-generation sequencing, combined with improved bioinformatics tools, is practically feas...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2016.01.013

    authors： Huang W,Wang G,Lin H,Zhuge J,Nolan SM,Vail E,Dimitrova N,Fallon JT

    更新日期：2016-05-01 00:00:00

  • Trends in nalidixic acid resistance in nontyphoidal Salmonella isolated from 1999 to 2002: decreased susceptibility to 6 fluoroquinolones.

    abstract：:We determined the resistance to nalidixic acid and 6 fluoroquinolones of 771 nontyphoidal Salmonella strains isolated from humans between 1999 and 2002. A total of 22 different serotypes were identified among the Salmonella isolates, the most common being Salmonella Enteritidis (79%) and Salmonella Derby (8%). Resista...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2005.02.013

    authors： Rodriguez-Avial I,Rodriguez-Avial C,López O,Picazo JJ

    更新日期：2005-07-01 00:00:00

  • Fluconazole in combination with flucytosine in the treatment of fluconazole-resistant Candida infections.

    abstract：:Two patients with renal failure and fluconazole resistant but flucytosine susceptible Candida deep infection were successfully treated with the combination of the two drugs. In vitro susceptibility, determined by microdilution and time-kill methods, and fungostatic activity of the serum showed an additive interaction ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/s0732-8893(03)00064-6

    authors： Girmenia C,Venditti M,Martino P

    更新日期：2003-07-01 00:00:00

  • Evaluation of the fully automated AIX1000 rapid plasma reagin system compared to a manual plasma reagin testing method for the diagnosis of syphilis.

    abstract：:The analytical performance of the FDA-cleared AIX1000 automated RPR testing platform was evaluated in comparison to manual RPR card testing. Eight hundred thirty-three patient serum samples were analyzed, 87 samples were positive by the AIX1000, 108 were positive by the manual test method; overall agreement between me...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2020.115081

    authors： Pyden A,Kang AD,Amato S,Dutta S,Berg G,Riedel S

    更新日期：2020-08-01 00:00:00

  • Evaluation of 3 automated real-time PCR (Xpert C. difficile assay, BD MAX Cdiff, and IMDx C. difficile for Abbott m2000 assay) for detecting Clostridium difficile toxin gene compared to toxigenic culture in stool specimens.

    abstract：:We evaluated the performance of the 3 automated systems (Cepheid Xpert, BD MAX, and IMDx C. difficile for Abbott m2000) detecting Clostridium difficile toxin gene compared to toxigenic culture. Of the 254 stool specimens tested, 87 (48 slight, 35 moderate, and 4 heavy growth) were toxigenic culture positive. The overa...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2015.05.005

    authors： Yoo J,Lee H,Park KG,Lee GD,Park YG,Park YJ

    更新日期：2015-09-01 00:00:00

  • Evaluation of antimicrobial susceptibility for beta-lactams using the Etest method against clinical isolates from 100 medical centers in Japan (2006).

    abstract：:This antimicrobial resistance surveillance study was performed in 100 medical centers. Susceptibility testing (Etest; AB BIODISK, Solna, Sweden) of 9152 strains including Escherichia coli (991 strains), Klebsiella spp. (1000 strains), Enterobacter spp. (971 strains), Citrobacter spp. (803 strains), indole-positive Pro...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2007.08.006

    authors： Ishii Y,Tateda K,Yamaguchi K,Japan Antimicrobial Resistance Surveillance Participants Group (JARS).

    更新日期：2008-02-01 00:00:00

  • Methicillin-susceptible strains responsible for postoperative orthopedic infection are not selected by the use of cefazolin in prophylaxis.

    abstract：:Comparison of methicillin-susceptible Staphylococcus aureus (MSSA) isolates responsible for bone and joint infection (BJI, n=73) and nasal colonization (n=57) revealed similar prevalence of β-lactamase (blaZ) type A production, associated with cefazolin hydrolysis, suggesting that blaZ type A-carrying MSSA isolates im...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2015.11.013

    authors： Trouillet-Assant S,Valour F,Mouton W,Martins-Simões P,Lustig S,Laurent F,Ferry T,Lyon BJI study group.

    更新日期：2016-03-01 00:00:00

  • Comparison of two commercial systems (Vitek and MicroScan-WalkAway) for antimicrobial susceptibility testing of Pseudomonas aeruginosa isolates from cystic fibrosis patients.

    abstract：:Antimicrobial susceptibility testing of cystic fibrosis (CF) isolates of Pseudomonas aeruginosa is difficult because the organisms are often mucoid and slow-growing. This study of 498 CF strains examined the correlation of results derived from two commonly used commercial systems (Vitek, MicroScan-WalkAway) with a ref...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章,多中心研究

    doi：10.1016/s0732-8893(01)00234-6

    authors： Burns JL,Saiman L,Whittier S,Krzewinski J,Liu Z,Larone D,Marshall SA,Jones RN

    更新日期：2001-04-01 00:00:00

  • Evaluation of light diagnostics SimulFluor HSV/VZV immunofluorescence assay.

    abstract：:In this study, we evaluated the Light Diagnostics SimulFluor HSV/VZV Immunofluorescence (SIM) assay. A total of 167 specimens yielded 21 VZV and 55 HSV true positive test results. The sensitivity/specificity of the SIM assay for VZV and HSV were, respectively, 100/98 and 62/99. These results were comparable to the res...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/s0732-8893(99)00078-4

    authors： Scicchitano LM,Shetterly B,Bourbeau PP

    更新日期：1999-11-01 00:00:00

  • Changes in the balance between Treg and Th17 cells in patients with chronic hepatitis B.

    abstract：:The purpose of this study was to explore the role of Treg cells, Th17 cells and cytokines associated with Treg/Th17 differentiation in the occurrence, development and outcome of chronic hepatitis B (CHB). To do so, we detected populations of Treg and Th17 cells and their associated cytokines in the peripheral blood of...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2013.04.026

    authors： Su ZJ,Yu XP,Guo RY,Ming DS,Huang LY,Su ML,Deng Y,Lin ZZ

    更新日期：2013-08-01 00:00:00

  • The central nervous system and infection by Candida species.

    abstract：:In this paper we have reviewed the main clinico-pathologic disease groups of neurocandidiasis: the microabscesses, the macroabscesses, and the meningitis. Special attention has been paid to the predisposing conditions for the appearance of neurocandidiasis, the neuroimaging techniques, and the study of the cerebrospin...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章,评审

    doi：10.1016/s0732-8893(00)00140-1

    authors： Sánchez-Portocarrero J,Pérez-Cecilia E,Corral O,Romero-Vivas J,Picazo JJ

    更新日期：2000-07-01 00:00:00

  • Identification of genetic recombination between Acinetobacter species based on multilocus sequence analysis.

    abstract：:During multilocus sequence analysis of Acinetobacter calcoaceticus-Acinetobacter baumannii complex, we identified the evidence of recent genetic recombination between 2 Acinetobacter species. While 3 isolates belonged to A. nosocomialis based on 16S rRNA, gyrB, fusA, gdhB, and rplB gene sequences, they showed close re...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2012.03.027

    authors： Kim DH,Park YK,Choi JY,Ko KS

    更新日期：2012-07-01 00:00:00

  • Comparison of the performance of IFA, CFA, and ELISA assays for the serodiagnosis of acute Q fever by quality assessment.

    abstract：:The indirect immunofluorescence assay (IFA) is considered the reference method for diagnosing Q fever, but serology is also performed by complement fixation assay (CFA) or enzyme-linked immunosorbent assay (ELISA). However, comparability between these assays is not clear, and therefore a quality assessment was perform...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2012.09.001

    authors： Herremans T,Hogema BM,Nabuurs M,Peeters M,Wegdam-Blans M,Schneeberger P,Nijhuis C,Notermans DW,Galama J,Horrevorts A,van Loo IH,Vlaminckx B,Zaaijer HL,Koopmans MP,Berkhout H,Socolovschi C,Raoult D,Stenos J,Nicholson W

    更新日期：2013-01-01 00:00:00

  • Hemophilus aphrophilus vertebral osteomyelitis: a case report and literature review.

    abstract：:We report a 60-yr-old diabetic female who developed vertebral osteomyelitis and discitis due to Hemophilus aphrophilus, and review three previously reported cases of this uncommon infection. Predisposing factors, clinical features, and treatment of H. aphrophilus vertebral osteomyelitis are discussed. ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章,评审

    doi：10.1016/0732-8893(87)90171-4

    authors： Gribble MJ,Hunter T

    更新日期：1987-11-01 00:00:00

  • Colonization and microbiology of the motile enterococci in a patient population.

    abstract：:The motile enterococci with the vanC gene have intrinsic low-level resistance to vancomycin, but have not been implicated in a nosocomial outbreak. We determined the colonization rate of motile enterococci in hospitalized and nonhospitalized patients. Perianal or stool specimens were cultured in Enterococcosel broth s...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/s0732-8893(98)00052-2

    authors： Van Horn KG,Rodney KM

    更新日期：1998-08-01 00:00:00

  • Occurrence of single-point gyrA mutations among ciprofloxacin-susceptible Escherichia coli isolates causing urinary tract infections in Latin America.

    abstract：:To detect if isolates susceptible to quinolones already carry mutations in the gyrA and parC genes, we selected 12 ciprofloxacin-susceptible Escherichia coli strains collected from patients with urinary tract infections in Latin America in 1998, as part of ongoing SENTRY Antimicrobial Surveillance Program. The isolate...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/s0732-8893(99)00121-2

    authors： Gales AC,Gordon KA,Wilke WW,Pfaller MA,Jones RN

    更新日期：2000-01-01 00:00:00

  • Antigen-specific CD4- and CD8-positive signatures in different phases of Mycobacterium tuberculosis infection.

    abstract：:Current diagnostic standards for Mycobacterium tuberculosis (MTB) infection do not distinguish between active and latent tuberculosis (TB). To identify specific biomarkers characterizing the different forms of TB infection, we investigated in parallel with the QuantiFERON -TB Gold In-Tube (QFT-IT) the use of flow cyto...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2012.11.023

    authors： Nikolova M,Markova R,Drenska R,Muhtarova M,Todorova Y,Dimitrov V,Taskov H,Saltini C,Amicosante M

    更新日期：2013-03-01 00:00:00

  • Elution kinetics, antimicrobial activity, and mechanical properties of 11 different antibiotic loaded acrylic bone cement.

    abstract：:Antibiotic-loaded acrylic bone cements (ALABC) spacers are routinely used in the treatment of prosthetic joint infections. The objectives of our study were to evaluate different ALABC for elution kinetics, thermal stability, and mechanical properties. A 10 or 20% mixture (w/w) beads of medium viscosity bone cement (De...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2013.09.014

    authors： Gálvez-López R,Peña-Monje A,Antelo-Lorenzo R,Guardia-Olmedo J,Moliz J,Hernández-Quero J,Parra-Ruiz J

    更新日期：2014-01-01 00:00:00

  • Evaluation of the efficacy of a posaconazole and anidulafungin combination in a murine model of pulmonary aspergillosis due to infection with Aspergillus fumigatus.

    abstract：:Posaconazole (PSC) in combination with anidulafungin (AFG) was evaluated in a murine model of pulmonary aspergillosis. Immunosuppressed animals were infected via the nasal cavity with 2 different A. fumigatus strains. The animals received PSC (oral, 20mg/kg per day) and/or AFG (i.p., 10mg/kg per day) for 7days. On Day...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2017.10.004

    authors： Bedin Denardi L,Pantella Kunz de Jesus F,Keller JT,Weiblen C,de Azevedo MI,Oliveira V,Morais Santurio J,Hartz Alves S

    更新日期：2018-01-01 00:00:00

  • Comparison of the microbiological characteristics and virulence factors of ST131 and non-ST131 clones among extended-spectrum β-lactamase-producing Escherichia coli causing bacteremia.

    abstract：:We evaluated the molecular epidemiology and microbiological characteristics of extended-spectrum β-lactamase-producing Escherichia coli (ESBL-EC) isolates that cause bacteremia in Korean hospitals, focusing especially on ST131. Our data suggest that ST131 isolates possessed more virulence traits and showed more multid...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章,多中心研究

    doi：10.1016/j.diagmicrobio.2015.10.015

    authors： Cha MK,Kang CI,Kim SH,Cho SY,Ha YE,Wi YM,Chung DR,Peck KR,Song JH,Korean Network for Study on Infectious Diseases (KONSID).

    更新日期：2016-02-01 00:00:00

  • Histoplasma capsulatum fungemia documented using the DuPont Isolator System.

    abstract：:A patient with disseminated histoplasmosis is described in whom blood cultures were negative using conventional media but positive for Histoplasma capsulatum using the DuPont Isolator system. Fungal blood culture methods are discussed. ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(84)90022-1

    authors： Wheat LJ,Bartlett M

    更新日期：1984-01-01 00:00:00

  • Age-related frequency of penicillin resistance of oral Veillonella.

    abstract：:Veillonella spp. are early colonizing inhabitants in the mouth. As part of studies on penicillin resistance among oral indigenous anaerobic microbiota in childhood, the aim of the present longitudinal study was to examine the emergence of resistant strains in Veillonella populations. Altogether 305 Veillonella isolate...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/s0732-8893(03)00082-8

    authors： Nyfors S,Könönen E,Bryk A,Syrjänen R,Jousimies-Somer H

    更新日期：2003-08-01 00:00:00

  • High prevalence of virulent Helicobacter pylori strains in symptomatic Bulgarian patients.

    abstract：:The aim of the study was to evaluate the prevalence of main virulence genes in Helicobacter pylori strains from 116 patients with peptic ulcers (41 cases) and nonulcer diseases (75) by polymerase chain reaction (PCR) with pure cultures and to compare the results with those by multiplex PCR in 39 H. pylori-positive gas...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2009.04.005

    authors： Boyanova L,Markovska R,Yordanov D,Marina M,Ivanova K,Panayotov S,Gergova G,Mitov I

    更新日期：2009-08-01 00:00:00

  • Evaluation of a sequential enzyme immunoassay testing algorithm for Lyme disease demonstrates lack of test independence but high diagnostic specificity.

    abstract：:To diagnose Lyme disease, a two-tier testing algorithm is used in which supplemental IgM and IgG immunoblots to detect antibody to Borrelia burgdorferi are reflexively performed if a first-tier assay, such as a whole-cell sonicate-based enzyme immunoassay (WCS EIA), is reactive. Recent data suggest that equal specific...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2018.02.006

    authors： Wormser GP,Molins CR,Levin A,Lipsett SC,Nigrovic LE,Schriefer ME,Branda JA

    更新日期：2018-07-01 00:00:00

  • Evaluation of Brucella dipstick assay for the diagnosis of acute brucellosis.

    abstract：:The diagnostic value of the dipstick assay was evaluated by comparison with Rose Bengal (RB), serum aglutination tests (SAT) and 2 mercaptoethanol test (2-ME) on consecutive serum samples submitted because of suspicion of brucellosis. Serum samples of 232 patients with suspected brucellosis that were submitted for lab...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/s0732-8893(02)00456-x

    authors： Altuglu I,Zeytinoğlu A,Bilgic A,Kamcioglu S,Karakartal G,Smits H

    更新日期：2002-11-01 00:00:00

  • The emergence of Staphylococcus epidermidis simultaneously nonsusceptible to linezolid and teicoplanin in China.

    abstract：:Two linezolid-resistant and teicoplanin-intermediate Staphylococcus epidermidis strains were isolated from blood cultures in China. The 2 S. epidermidis strains were methicillin-resistant and showed multidrug-resistance patterns; in addition, population analysis profiling/area under the curve (PAP/AUC) result showed h...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2019.114956

    authors： Liu C,Chen C,Ye Y,Li X,Sun J,Xu L,Ming L

    更新日期：2020-02-01 00:00:00

  • Clinical evaluation of a new single-tube multiplex reverse transcription PCR assay for simultaneous detection of 11 respiratory viruses, Mycoplasma pneumoniae and Chlamydia in hospitalized children with acute respiratory infections.

    abstract：:Respiratory Pathogen 13 Detection Kit (13× kit) is able to simultaneously detect 11 respiratory viruses, Mycoplasma pneumoniae (MP) and Chlamydia in a single reaction. Using 572 Nasopharyngeal aspirates collected from hospitalized children, the clinical performance of 13× kit for detecting 11 respiratory viruses was e...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2017.03.010

    authors： Zhao MC,Li GX,Zhang D,Zhou HY,Wang H,Yang S,Wang L,Feng ZS,Ma XJ

    更新日期：2017-06-01 00:00:00

  • Rapid detection of coinfections by Trichomonas vaginalis, Mycoplasma hominis, and Ureaplasma urealyticum by a new multiplex polymerase chain reaction.

    abstract：:We developed a multiplex polymerase chain reaction (M-PCR) assay to simultaneously detect Trichomonas vaginalis, Mycoplasma hominis, and Ureaplasma urealyticum. The test is extremely specific and has a sensitivity of 10 cells for T. vaginalis and U. urealyticum and of 1 cell for M. hominis. The technique was validated...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2009.12.022

    authors： Diaz N,Dessì D,Dessole S,Fiori PL,Rappelli P

    更新日期：2010-05-01 00:00:00

  • In vitro susceptibility of 33 clinical case isolates of Xanthomonas maltophilia. Inconsistent correlation of agar dilution and of disk diffusion test results.

    abstract：:Xanthomonas maltophilia is an emerging nosocomial pathogen, possibly selected by a changing antimicrobial usage and patient population. In the present study, we tested the susceptibility of 33 recent clinical case isolates to 12 commonly employed antimicrobials. Trimethoprim-sulfamethoxazole (1:19 ratio) and doxycycli...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(91)90072-n

    authors： Hohl P,Frei R,Aubry P

    更新日期：1991-09-01 00:00:00