Antigen-specific CD4- and CD8-positive signatures in different phases of Mycobacterium tuberculosis infection.

Abstract:

:Current diagnostic standards for Mycobacterium tuberculosis (MTB) infection do not distinguish between active and latent tuberculosis (TB). To identify specific biomarkers characterizing the different forms of TB infection, we investigated in parallel with the QuantiFERON -TB Gold In-Tube (QFT-IT) the use of flow cytometry measuring CD4 and CD8 MTB-specific immune response in 17 active-TB patients, 21 health care workers (HCW), 14 recent contacts of TB patients (RC-TB), and 10 bacille Calmette Guerin (BCG)-vaccinated healthy controls (BCG-HC). A correlation (r = 0.4526, P = 0.0002) was found only between the amount of IFN-γ measured by QFT-IT and the frequency of CD4+/CD69+/IFN-γ+ T cells. The frequency of CD4+/CD69+/IFNγ+ responding T cells was higher in active-TB patients (0.254 ± 0.336%, P < 0.01) compared to the other groups. The response of QFT-IT antigen-specific CD8+/CD69+/IFNγ+ T cells was significantly higher in RC-TB (0.245 ± 0.305%, P < 0.05) compared to the other study groups.

authors

Nikolova M,Markova R,Drenska R,Muhtarova M,Todorova Y,Dimitrov V,Taskov H,Saltini C,Amicosante M

doi

10.1016/j.diagmicrobio.2012.11.023

subject

Has Abstract

pub_date

2013-03-01 00:00:00

pages

277-81

issue

3

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(12)00506-8

journal_volume

75

pub_type

杂志文章
  • Fusarium verticillioides fungemia in a liver transplantation patient: successful treatment with voriconazole.

    abstract::Fusarium is an opportunistic fungal pathogen which is emerging as a significant cause of morbidity and mortality in immunocompromised hosts. We present a rare case of F. verticillioides fungemia that occurred in a patient who underwent a second orthotopic liver transplantation for chronic rejection and completely resp...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2011.08.024

    authors: Cocchi S,Codeluppi M,Venturelli C,Bedini A,Grottola A,Gennari W,Cavrini F,Di Benedetto F,De Ruvo N,Rumpianesi F,Gerunda GE,Guaraldi G

    更新日期:2011-12-01 00:00:00

  • Helicobacter pylori growth stimulation by adrenaline detected by two methods.

    abstract::Our aim was to assess growth effects of adrenaline on 32 Helicobacter pylori strains on 2.5% blood agar. By agar-well diffusion and disk diffusion methods, concentration-dependent growth stimulation occurred in ≤50.0% and 36.8% of the strains, respectively. Growth inhibition was less frequent. Most strains were adrena...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2018.08.004

    authors: Boyanova L,Markovska R,Mitov I

    更新日期:2019-01-01 00:00:00

  • In vitro susceptibility and synergy studies of Aspergillus species to conventional and new agents.

    abstract::In vitro susceptibility data using a macrodilution broth method on greater than 100 isolates of Aspergillus spp. are presented. For amphotericin B (Amp B) (n = 105), 67% had minimum inhibitory concentrations (MICs) less than or equal to 2 micrograms/ml, and 90% had MICs less than or equal to 4 micrograms/ml; for 5-flu...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/0732-8893(92)90053-v

    authors: Denning DW,Hanson LH,Perlman AM,Stevens DA

    更新日期:1992-01-01 00:00:00

  • Infective endocarditis and osteomyelitis caused by Cellulomonas: a case report and review of the literature.

    abstract::Cellulomonas spp. are often believed to be of low virulence and have never been reported as a pathogen causing human disease before. We report the first case of endocarditis caused by Cellulomonas and complicated with osteomyelitis of the lumbar spine in a 78-year-old woman. General weakness and aggravated lower back ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章,评审

    doi:10.1016/j.diagmicrobio.2009.06.001

    authors: Lai PC,Chen YS,Lee SS

    更新日期:2009-10-01 00:00:00

  • Production of extracellular enzymes and cytotoxicity by Vibrio vulnificus.

    abstract::Thirty-three strains of Vibrio vulnificus of clinical and environmental origin were examined for production of 12 extracellular enzymes of potential importance to the virulence of this bacterium. Strains of Vibrio vulnificus were consistent in their production of protease, mucinase, lipase, chondroitinase, hyaluronida...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/0732-8893(86)90112-4

    authors: Oliver JD,Wear JE,Thomas MB,Warner M,Linder K

    更新日期:1986-07-01 00:00:00

  • Cross-over assessment of serum bactericidal activity of moxifloxacin and levofloxacin versus penicillin-susceptible and penicillin-resistant Streptococcus pneumoniae in healthy volunteers.

    abstract::We compared the serum bactericidal activity (SBA) of moxifloxacin and levofloxacin against penicillin-susceptible and penicillin-resistant Streptococcus pneumoniae in 12 healthy volunteers. Each subject received 3 days of oral moxifloxacin 400 mg daily and levofloxacin 750 mg daily, respectively, with a 2- to 4-week w...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 临床试验,杂志文章

    doi:10.1016/j.diagmicrobio.2007.02.006

    authors: Hart D,Weinstein MP

    更新日期:2007-07-01 00:00:00

  • In vitro activity and killing effect of the synthetic hybrid cecropin A-melittin peptide CA(1-7)M(2-9)NH(2) on methicillin-resistant nosocomial isolates of Staphylococcus aureus and interactions with clinically used antibiotics.

    abstract::The in vitro activity of CA(1-7)M(2-9)NH(2), a 15-residue synthetic hybrid peptide derived from the sequences of cecropin A and melittin, alone and in combination with amoxicillin-clavulanate, imipenem, clarithromycin, ciprofloxacin, rifampin, and vancomycin, was investigated against 40 nosocomial isolates of methicil...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2004.02.008

    authors: Giacometti A,Cirioni O,Kamysz W,D'Amato G,Silvestri C,Simona Del Prete M,Lukasiak J,Scalise G

    更新日期:2004-07-01 00:00:00

  • Acinetobacter species isolates from a range of environments: species survey and observations of antimicrobial resistance.

    abstract::Acinetobacter species isolates from a range of environments, including soil, were investigated. We determined 16S rRNA and rpoB gene sequences for species identification and performed tests of antimicrobial resistance susceptibility. Twenty-nine of the isolates (8 from soil and 21 from life environment) belonged to th...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2012.06.023

    authors: Choi JY,Kim Y,Ko EA,Park YK,Jheong WH,Ko G,Ko KS

    更新日期:2012-10-01 00:00:00

  • Analytical and clinical validation of novel real-time reverse transcriptase-polymerase chain reaction assays for the clinical detection of swine-origin H1N1 influenza viruses.

    abstract::During the early stages of the 2009/2010 swine-origin H1N1 influenza A (S-OIV H1N1 FluA) outbreak, the development and validation of sensitive and specific detection methods were a priority for rapid and accurate diagnosis. Between May and June 2009, 2 real-time reverse transcriptase-polymerase chain reaction (rRT-PCR...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2010.09.020

    authors: Duncan C,Guthrie JL,Tijet N,Elgngihy N,Turenne C,Seah C,Lau R,McTaggart L,Mallo G,Perusini S,Rebbapragada A,Melano R,Low DE,Farrell D,Guyard C

    更新日期:2011-02-01 00:00:00

  • Nontyphoid salmonella urinary tract infections.

    abstract::Urinary tract infection caused by nontyphoid salmonella is rare. A case is described in a patient with acquired immunodeficiency syndrome who had multiple recurrences. A review of cases in which salmonella involved the urinary tract indicates that this infection usually occurs in patients who are structurally or immun...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/0732-8893(87)90016-2

    authors: Eng RH,Smith SM,Kloser P

    更新日期:1987-03-01 00:00:00

  • Performance of Directigen flu A+B enzyme immunoassay and direct fluorescent assay for detection of influenza infection during the 2004-2005 season.

    abstract::Early diagnosis of influenza infection is needed to optimize the benefit of prescribing antiviral drugs. However, the accuracy of rapid tests is highly variable. This study evaluated the performance of Directigen flu A+B enzyme immunoassay (EIA) and direct fluorescent assay (DFA) during the 2004-2005 influenza season....

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2007.03.011

    authors: Rahman M,Kieke BA,Vandermause MF,Mitchell PD,Greenlee RT,Belongia EA

    更新日期:2007-08-01 00:00:00

  • Comparison of the efficacy and adverse effect profile of cefotaxime and ceftriaxone in ICU patients with susceptible infections.

    abstract::In a prospective randomized double-blind trial, the efficacy and safety of cefotaxime and ceftriaxone were compared in intensive care unit (ICU) patients with serious infections requiring systemic antimicrobial therapy. Patients were randomly assigned to receive either cefotaxime 1 g i.v. t.i.d. or ceftriaxone 2 g eve...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1016/0732-8893(92)90062-x

    authors: Thomas P,Daly S,Misan G,Steele T

    更新日期:1992-01-01 00:00:00

  • Occurrence of the vanA and vanC2/C3 genes in Enterococcus species isolated from poultry sources in Malaysia.

    abstract::Enterococcus species isolated from poultry sources were characterized for their resistance to antibiotics, plasmid content, presence of van genes and their diversity by randomly amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR). The results showed that all isolates were multi-resistance to the antibiotics...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/s0732-8893(01)00222-x

    authors: Radu S,Toosa H,Rahim RA,Reezal A,Ahmad M,Hamid AN,Rusul G,Nishibuchi M

    更新日期:2001-03-01 00:00:00

  • Risk factors for acquisition of vancomycin-resistant enterococci among hematology-oncology patients.

    abstract::The incidence of VRE has increased dramatically and hematology-oncology patients are at high risk for acquisition of colonization and development of infection. Therefore, we performed a prospective cohort study to determine risk factors for VRE acquisition among hematology-oncology patients. Patients admitted to a sin...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/s0732-8893(02)00392-9

    authors: Suntharam N,Lankford MG,Trick WE,Peterson LR,Noskin GA

    更新日期:2002-07-01 00:00:00

  • Two-year surveillance on fluconazole susceptibility of Candida spp isolates in a general and university hospital in Rome.

    abstract::Fluconazole susceptibility was tested in 385 clinical yeast isolates (285 Candida albicans, 38 C. glabrata, 31 C. tropicalis, 31 other Candida subsp.) using the agar disk diffusion test. Yeasts were collected from specimens obtained from outpatients (69) and inpatients (intensive care unit: 79 isolates, major burn uni...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/s0732-8893(01)00280-2

    authors: Testore GP,Falco F,Sarrecchia C,Sordillo P,Bontempo G,Andreoni M

    更新日期:2001-09-01 00:00:00

  • Comparative in vitro activity of cefoxitin, cefotaxime alone, and in combination with desacetylcefotaxime against the Bacteroides species.

    abstract::The agar dilution method was used to determine the inhibitory activity of cefotaxime (CTX) alone, desacetylcefotaxime (dCTX) alone, CTX plus dCTX, and cefoxitin against 74 clinical isolates of the Bacteroides species recovered from diabetic patients with foot ulcers. The study concluded that the addition of dCTX to CT...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/0732-8893(89)90043-6

    authors: Canawati HN

    更新日期:1989-01-01 00:00:00

  • Assessing next-generation sequencing and 4 bioinformatics tools for detection of Enterovirus D68 and other respiratory viruses in clinical samples.

    abstract::We used 4 different bioinformatics algorithms to evaluate the application of a metagenomic shot-gun sequencing method in detection of Enterovirus D68 and other respiratory viruses in clinical specimens. Our data supported that next-generation sequencing, combined with improved bioinformatics tools, is practically feas...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2016.01.013

    authors: Huang W,Wang G,Lin H,Zhuge J,Nolan SM,Vail E,Dimitrova N,Fallon JT

    更新日期:2016-05-01 00:00:00

  • In vitro activity of tedizolid against staphylococci isolated from prosthetic joint infections.

    abstract::We determined the MIC and minimum biofilm bactericidal concentration (MBBC) of tedizolid and vancomycin against 97 isolates of Staphylococcus aureus and 74 isolates of Staphylococcus epidermidis associated with prosthetic joint infection. All isolates were vancomycin susceptible in the planktonic state; all staphyloco...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2016.01.008

    authors: Schmidt-Malan SM,Greenwood Quaintance KE,Karau MJ,Patel R

    更新日期:2016-05-01 00:00:00

  • Ceftriaxone pharmacokinetics by new simple and sensitive ultra-high-performance liquid chromatography method.

    abstract::Ceftriaxone is a cephalosporin antibiotic with a potent antimicrobial activity and excellent penetration in most body fluids such as pleural, peritoneal, spinal and brain. These facts contribute to the application of ceftriaxone in the treatment of bacterial peritonitis, an abdominal disorder in veterinary medicine, w...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2017.02.012

    authors: Campos ML,de Moura Alonso J,Dos Santos Martins E,Oliveira JA,Hussni CA,Peccinini RG

    更新日期:2017-05-01 00:00:00

  • Direct detection and amplification of Helicobacter pylori ribosomal 16S gene segments from gastric endoscopic biopsies.

    abstract::Helicobacter pylori is an organism thought to play an important causative role in gastritis and peptic ulcer diseases. We have designed an RNA dot blot assay for the detection of H. pylori, using as probe a synthetic oligonucleotide complementary to its 16S rRNA. We have also used oligonucleotide primers, complementar...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/0732-8893(90)90079-b

    authors: Hoshina S,Kahn SM,Jiang W,Green PH,Neu HC,Chin N,Morotomi M,LoGerfo P,Weinstein IB

    更新日期:1990-11-01 00:00:00

  • Comparative in vitro pharmacodynamics of imipenem and meropenem against ATCC strains of Escherichia coli, Staphylococcus aureus and Bacteroides fragilis.

    abstract::We evaluated the pharmacodynamics of imipenem and meropenem, utilizing time-kill studies over a concentration/MIC (C/MIC) range of 0.0625-1024 for E. coli ATCC 35218 (EC) and S. aureus ATCC 29213 (SA) and from 0.125-512 for B. fragilis ATCC 25285 (BF). Area under the time-kill curves were converted to percent response...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/s0732-8893(00)00219-4

    authors: White RL,Friedrich LV,Manduru M,Mihm LB,Bosso JA

    更新日期:2001-01-01 00:00:00

  • Evaluation of light diagnostics SimulFluor HSV/VZV immunofluorescence assay.

    abstract::In this study, we evaluated the Light Diagnostics SimulFluor HSV/VZV Immunofluorescence (SIM) assay. A total of 167 specimens yielded 21 VZV and 55 HSV true positive test results. The sensitivity/specificity of the SIM assay for VZV and HSV were, respectively, 100/98 and 62/99. These results were comparable to the res...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/s0732-8893(99)00078-4

    authors: Scicchitano LM,Shetterly B,Bourbeau PP

    更新日期:1999-11-01 00:00:00

  • CMY-2, CMY-8b, and DHA-1 plasmid-mediated AmpC β-lactamases among clinical isolates of Escherichia coli and Klebsiella pneumoniae from a university hospital, Thailand.

    abstract::Between February 2005 and January 2006 in Srinagarind Hospital, Thailand, 44 from 1730 isolates (2.5%) of Escherichia coli and 8 from 982 isolates (0.8%) of Klebsiella pneumoniae were found to produce plasmid-mediated AmpC β-lactamases (pAmpCs) as detected by a cefoxitin-Hodge test followed by a multiplex polymerase c...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2010.06.014

    authors: Singtohin S,Chanawong A,Lulitanond A,Sribenjalux P,Auncharoen A,Kaewkes W,Songsri J,Pienthaweechai K

    更新日期:2010-11-01 00:00:00

  • Direct detection of Mycobacterium tuberculosis in clinical specimens using single-tube biotinylated nested polymerase chain reaction-enzyme linked immunoassay (PCR-ELISA).

    abstract::A biotinylated single-tube nested polymerase chain reaction (PCR) assay with microwell hybridization assay (bPCR-ELISA) was developed for detection of Mycobacterium tuberculosis in clinical specimens. A total of 659 specimens (601 respiratory specimens and 58 nonrespiratory specimens) were collected for evaluation usi...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2003.11.006

    authors: Yam WC,Cheng VC,Hui WT,Wang LN,Seto WH,Yuen KY

    更新日期:2004-04-01 00:00:00

  • Identification of Staphylococcus spp. and detection of mecA by an oligonucleotide array.

    abstract::Phenotypic identification of coagulase-negative staphylococci (CoNS) is difficult and many staphylococcal species carry mecA. This study developed an array that was able to detect mecA and identify 30 staphylococcal species by targeting the internal transcribed spacer regions. A total of 129 target reference strains (...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2016.06.003

    authors: Han HW,Chang HC,Chang TC

    更新日期:2016-09-01 00:00:00

  • Occurrence of single-point gyrA mutations among ciprofloxacin-susceptible Escherichia coli isolates causing urinary tract infections in Latin America.

    abstract::To detect if isolates susceptible to quinolones already carry mutations in the gyrA and parC genes, we selected 12 ciprofloxacin-susceptible Escherichia coli strains collected from patients with urinary tract infections in Latin America in 1998, as part of ongoing SENTRY Antimicrobial Surveillance Program. The isolate...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/s0732-8893(99)00121-2

    authors: Gales AC,Gordon KA,Wilke WW,Pfaller MA,Jones RN

    更新日期:2000-01-01 00:00:00

  • The distribution and drug susceptibilities of clinical Candida species in TSARY 2014.

    abstract::The species distribution and drug susceptibilities of 1106 Candida isolates collected in Taiwan Surveillance of Antimicrobial Resistance of Yeasts (TSARY) in 2014 were determined. Candida albicans is still the dominant species, accounting for 35.9%, followed by 28.3% C. glabrata, 26.6% C. tropicalis, 5.2% C. parapsilo...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章,多中心研究

    doi:10.1016/j.diagmicrobio.2016.09.009

    authors: Zhou ZL,Lin CC,Chu WL,Yang YL,Lo HJ,TSARY Hospitals.

    更新日期:2016-12-01 00:00:00

  • Effects of feeding lactobacillus GG on lethal irradiation in mice.

    abstract::Mice exposed to 1400 rads of total body irradiation experienced 80%-100% mortality in 2 wk. Bacteremia was demonstrated in all dead animals. Feeding Lactobacillus GG strain reduced Pseudomonas bacteremia and prolonged survival time in animals colonized with this organism. In animals not colonized with Pseudomonas, fee...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/0732-8893(87)90063-0

    authors: Dong MY,Chang TW,Gorbach SL

    更新日期:1987-05-01 00:00:00

  • Corynebacterium jeikeium (group JK diphtheroids) endocarditis. A report of five cases.

    abstract::Corynebacterium jeikeium is the name recently given to a group of coryneform rods, formerly known as the JK group, and associated with a wide range of serious infections. We describe five patients with C. jeikeium endocarditis observed in two Belgian hospitals over a period of 5 years. In three cases, the infection oc...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/0732-8893(89)90025-4

    authors: Vanbosterhaut B,Surmont I,Vandeven J,Wauters G,Vandepitte J

    更新日期:1989-05-01 00:00:00

  • Two cases of acute hepatitis after a serial knife stabbing.

    abstract::Three persons became victims of a serial knife stabbing in a fight. Three months later, one of the victims presented with acute hepatitis. On the fifth day of his admission to the hospital, another victim of this serial stabbing presented to our clinic with acute hepatitis B infection. The patients told that the first...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2012.07.009

    authors: Bodur H,Onguru P,Akıncı E,Kanyılmaz D,Yetkin A,Ozdemir B,But A,Eren S

    更新日期:2012-11-01 00:00:00