Abstract:
:We compared the performance of 8 Clostridium difficile enzyme immunoassays to cell cytotoxicity neutralization assay and toxigenic culture. The effect of strain type on assay performance was also examined. There were a total of 71 (14.4%) samples in which C. difficile was recovered; 58 (81.7%) of 71 were toxigenic. Compared to a composite reference standard of either C. difficile cytotoxin assay positive or toxigenic C. difficile culture positive, the sensitivities of these assays varied from 31.7% to 55.2%, while the specificities were excellent, ranging from 98.1% to 100%. Among the 57 C. difficile isolates, 30 (51.7%) were of the NAP1 genotype. Stool samples positive for the C. difficile NAP1 strain had a higher positivity rate for the toxin assays.
journal_name
Diagn Microbiol Infect Disjournal_title
Diagnostic microbiology and infectious diseaseauthors
René P,Frenette CP,Schiller I,Dendukuri N,Brassard P,Fenn S,Loo VGdoi
10.1016/j.diagmicrobio.2012.01.005subject
Has Abstractpub_date
2012-05-01 00:00:00pages
94-6issue
1eissn
0732-8893issn
1879-0070pii
S0732-8893(12)00025-9journal_volume
73pub_type
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