Abstract：
:Combining a polymerase chain reaction (PCR) test with bronchoscopy is frequently performed to allow a rapid diagnosis of smear-negative pulmonary tuberculosis (PTB). However, limited data are available concerning clinical judgment in patients with suspected PTB and AFB smear- and PCR-negative bronchial aspirates (BA). The present study evaluated the usefulness of whole-blood QuantiFERON-TB Gold In-Tube (QFT) testing in these patients. Of 166 patients with suspected PTB who had undergone bronchoscopy because of smear-negative sputum or inadequate sputum production, 93 (56%) were diagnosed with culture-positive PTB. Seventy-four patients were either AFB smear- or PCR-positive. In the 75 patients whose BA AFB smear and PCR results were both negative, 19 were finally diagnosed with PTB by culture. The QFT test had a negative predictive value of 91% for PTB. The QFT test may be useful for excluding PTB in patients with suspected PTB whose BA AFB smear and PCR results are both negative.
journal_name
Diagn Microbiol Infect Disjournal_title
Diagnostic microbiology and infectious diseaseauthors
Lee J,Lee SY,Yoo SS,Cha SI,Won DI,Park JY,Lee WK,Kim CHdoi
10.1016/j.diagmicrobio.2012.03.019subject
Has Abstractpub_date
2012-07-01 00:00:00pages
252-6issue
3eissn
0732-8893issn
1879-0070pii
S0732-8893(12)00111-3journal_volume
73pub_type
杂志文章abstract：:We characterized spa types, SCCmec types, and antimicrobial resistance patterns of 516 methicillin-resistant Staphylococcus aureus (MRSA) isolates, collected between 2011 and 2014 from nares and blood cultures of United States patients. Among nares isolates, 45 spa types were observed; 29.9% were t002/SCCmec II and 30...
journal_title：Diagnostic microbiology and infectious disease
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abstract：:The Inno LiPA Mycobacteria assay, based on PCR amplification of the 16-23S rRNA spacer region of Mycobacterium species, has been designed for identification of mycobacteria grown in culture media and discrimination between Mycobacterium tuberculosis complex, M. avium, M. intracellulare, M. kansasii, M. gordonae, M. xe...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/s0732-8893(02)00406-6
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abstract：:Serious Candida glabrata infections, which can be difficult to treat, are often treated with echinocandins. We compared in vitro the effects of high and low concentrations of 3 echinocandins (micafungin [MCF], caspofungin [CAS], and anidulafungin [ANF]), voriconazole (VRC), and amphotericin B (AmB), singly and VRC in ...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2011.11.004
更新日期：2012-03-01 00:00:00
abstract：:We compared the serum bactericidal activity (SBA) of moxifloxacin and levofloxacin against penicillin-susceptible and penicillin-resistant Streptococcus pneumoniae in 12 healthy volunteers. Each subject received 3 days of oral moxifloxacin 400 mg daily and levofloxacin 750 mg daily, respectively, with a 2- to 4-week w...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 临床试验,杂志文章
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更新日期：2007-07-01 00:00:00
abstract：:Clostridium difficile infections (CDI) is a leading cause of nosocomial infections worldwide. The changes in the epidemiology of CDI during the past years, including the appearance of new epidemic strains of C. difficile that cause CDI episodes with increased severity, have led to the development of molecular methods ...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
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abstract：:A 4 hr test for hydrolysis of L-pyrrolidonyl-beta-naphthylamide (PYR) was evaluated for its use in identifying group A beta-hemolytic streptococci, group D enterococci, and Staphylococcus haemolyticus. All of the group A streptococci and the enterococci tested were positive in the PYR test, whereas all of the nongroup...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
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abstract：:The Verigene Gram-positive blood culture assay (BC-GP) is a microarray-based rapid diagnostic test, which includes targets for 12 bacterial species and 3 resistance determinants. We prospectively compared the diagnostic accuracy of the BC-GP to routine microbiologic methods and evaluated the potential of the BC-GP for...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2014.09.025
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journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/s0732-8893(96)00126-5
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abstract：:We report a 60-yr-old diabetic female who developed vertebral osteomyelitis and discitis due to Hemophilus aphrophilus, and review three previously reported cases of this uncommon infection. Predisposing factors, clinical features, and treatment of H. aphrophilus vertebral osteomyelitis are discussed. ...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章,评审
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abstract：:A commercially available indirect fluorescent antibody (IFA) test was evaluated for the determination of measles-specific immunoglobulins G and M (MIgG, MIgM). The IFA test detected fourfold rises of MIgG in 34 of 35 (97%) cases of measles confirmed by complement fixation or hemagglutination inhibition. In determining...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/0732-8893(86)90068-4
更新日期：1986-04-01 00:00:00
abstract：:Molecular epidemiology allows us to know local HIV transmission and to design strategies of prevention. We studied 25 HIV newly diagnosed patients with the E138A mutation since the year 2010. Most transmission networks involved young and promiscuous men who have sex with men. Recent infection was only documented in pa...
journal_title：Diagnostic microbiology and infectious disease
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abstract：:A 14-year longitudinal survey of Streptococcus pneumoniae (18,911) strains by the SENTRY Antimicrobial Surveillance Program shows significant increases in resistance to amoxicillin/clavulanate (18.9%), penicillin at a MIC of ≥ 4 μg/mL (14.8%), and ceftriaxone (11.7%) among 2011 isolates. Fluoroquinolones (levofloxacin...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2012.08.024
更新日期：2013-01-01 00:00:00
abstract：:Outbreaks of rapidly growing mycobacterium (RGM) infections are increasingly being reported worldwide. Information about genetic relatedness of isolates obtained during outbreaks can provide opportunities for prompt intervention. Pulsed-field gel electrophoresis (PFGE) is expensive, time consuming, and labor intensive...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2006.01.006
更新日期：2006-06-01 00:00:00
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journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2014.12.008
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abstract：:Although there are many reports of the high diagnostic accuracy of commercially available serologic kits for Helicobacter pylori infection in Western countries, they rarely has been investigated in oriental population. Accordingly we examined their usefulness in 492 Japanese patients with dyspeptic symptoms. Diagnosti...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 临床试验,杂志文章
doi：10.1016/s0732-8893(99)00143-1
更新日期：2000-02-01 00:00:00
abstract：:Legionella pneumophila is responsible for Legionnaires' disease (LD). Its detection in both environmental and clinical samples is mainly performed by culture plate method which requires up to 10days to obtain results. Nowadays, there are commercial antibodies against this bacterium, but they have not been tested again...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2017.11.005
更新日期：2018-02-01 00:00:00
abstract：:The FiltraCheck-UT1 (FC) is a disposable colorimetric urine screen for bacteriuria that requires less than 1 min to perform. The results of the FC and two other urine screens, the Bac-T-Screen (BTS) and the Chemstrip LN test strip (LN), were compared with quantitative culture method. A total of 551 urine specimens wer...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/0732-8893(90)90019-r
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abstract：:A comparison was made of the discriminating power of the E-Test and conventional methods to distinguish different bacterial strains harboring plasmid-mediated extended-spectrum beta-lactamases. The E-Test provided quantitative data comparable to those of a broad range of concentrations in the agar dilution method, but...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
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journal_title：Diagnostic microbiology and infectious disease
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journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
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abstract：:Vitek® 2 (bioMérieux) is a widely used commercial antimicrobial susceptibility test (AST) system. AST-N244 card includes cephalothin as first-generation cephalosporin. We compared the cephalothin susceptibility results obtained with Vitek® 2 AST-N244 to those obtained by broth microdilution (BMD) and disk diffusion (D...
journal_title：Diagnostic microbiology and infectious disease
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journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
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更新日期：2007-07-01 00:00:00
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journal_title：Diagnostic microbiology and infectious disease
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journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
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journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
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journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
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abstract：:The emergence of the pandemic influenza virus A H1N1 has made fast and accurate diagnosis essential. However, few well-validated diagnostic techniques exist. The real-time RT-PCR developed by the Centers for Disease Control and Prevention (CDC) is the recommended technique. Our objective was to compare the CDC real-ti...
journal_title：Diagnostic microbiology and infectious disease
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abstract：PURPOSE:In this retrospective study, we aimed to investigate the value of FDG-PET/CT in the diagnosis of spondylodiscitis (SD), the significance of dual time point imaging (DTPI) for SD diagnosis and the worth of SUVmax data for distinguishing tuberculous vs. non-tuberculous SD. MATERIALS AND METHODS:32 patients with ...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
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更新日期：2016-07-01 00:00:00
abstract：:We used 4 different bioinformatics algorithms to evaluate the application of a metagenomic shot-gun sequencing method in detection of Enterovirus D68 and other respiratory viruses in clinical specimens. Our data supported that next-generation sequencing, combined with improved bioinformatics tools, is practically feas...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
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更新日期：2016-05-01 00:00:00
abstract：:An analysis was made on the basis of 5,389 isolates from 5,008 positive blood cultures of the relative yields from 10, 20, and 30 ml of blood. Average yields from cultures of 20 and 30 ml of blood were, respectively, 38 and 61% greater than that from 10 ml of blood. ...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/0732-8893(83)90039-1
更新日期：1983-06-01 00:00:00
