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Investigation of nosocomial respiratory infection due to Pseudomonas cepacia by arbitrarily primed polymerase chain reaction.

Abstract：

:We used DNA fingerprinting by the arbitrarily primed polymerase chain reaction (AP-PCR) technique for an epidemiologic investigation of Pseudomonas cepacia nosocomial isolates obtained from patients attending our hospital. This approach was compared with conventional phenotypic typing and pulsed-field gel electrophoresis (PFGE). The patterns of gel electrophoresis of the products of AP-PCR differed significantly according to differences in the concentration of Mg2+ and in pH. AP-PCR and PFGE was identical in their resolving power, as the two methods generated four different profiles and identified the same group of strains. The AP-PCR method constitutes an easy alternative to the well-established PFGE method.

journal_name

Diagn Microbiol Infect Dis

journal_title

Diagnostic microbiology and infectious disease

authors

Miyawaki H,Fujita J,Takigawa K,Negayama K,Yamagishi Y,Yamaji Y,Ouchi K,Nakazawa T,Kawanishi K,Takahara J

doi

10.1016/0732-8893(95)00176-x

subject

Has Abstract

pub_date

1995-11-01 00:00:00

pages

77-83

issue

3

eissn

0732-8893

issn

1879-0070

pii

0732-8893(95)00176-X

journal_volume

23

pub_type

杂志文章

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