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Application of a loop-mediated isothermal amplification method for the detection of pathogenic Leptospira.

Abstract：

:Leptospirosis is an emerging infectious disease, which is considered to be the most widespread zoonotic disease in the world. There are more than 230 known serovars in the genus Leptospira. A loop-mediated isothermal amplification (LAMP) assay for the rapid detection of pathogenic Leptospira spp. was developed and evaluated through amplification of the lipL41 gene coding for the outer membrane protein LipL41. The LAMP assay did not rely on the isolation and culture of leptospires, and no cross-reactivity was observed with other bacterial species. A SYBR Green I-based LAMP assay was also carried out for the real-time detection of DNA amplification. The lower detection limit of the LAMP assay was approximately 100 copies, which was the same as the polymerase chain reaction (PCR) and real-time PCR assays. The accuracy of the LAMP reaction was confirmed by restriction endonuclease analysis of the amplified product. The LAMP assay is easy to perform and inexpensive, and so may be applied in the rapid and specific diagnosis of Leptospira.

journal_name

Diagn Microbiol Infect Dis

journal_title

Diagnostic microbiology and infectious disease

authors

Lin X,Chen Y,Lu Y,Yan J,Yan J

doi

10.1016/j.diagmicrobio.2008.10.012

subject

Has Abstract

pub_date

2009-03-01 00:00:00

pages

237-42

issue

3

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(08)00446-X

journal_volume

63

pub_type

杂志文章

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