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Molecular analysis of archived blood slides reveals an atypical human Trypanosoma infection.

Abstract：

:In 2003, a 10-month-old Ghanaian boy recovered from a Trypanosoma brucei infection, although the patient was not treated with antitrypanosomal drugs. Only T. brucei gambiense and T. brucei rhodesiense are able to infect humans, causing human African trypanosomiasis. The disease is considered 100% fatal if left untreated. The identity of the trypanosome was determined by DNA extraction from the archived stained blood slides followed by sequential application of polymerase chain reactions (PCRs) that are specific for the order, subgenus, species and subspecies, followed by genotyping with microsatellite PCR. Molecular analysis indicated that the parasites observed in the patient's blood in 2003 belong to the T. brucei subspecies brucei, which is normally not infectious to humans. Next to the clinical message, this article provides technical information to extract successfully DNA from archived blood slides for subsequent molecular analysis and to identify a trypanosome by taxon-specific PCRs and microsatellite genotyping.

journal_name

Diagn Microbiol Infect Dis

journal_title

Diagnostic microbiology and infectious disease

authors

Deborggraeve S,Koffi M,Jamonneau V,Bonsu FA,Queyson R,Simarro PP,Herdewijn P,Büscher P

doi

10.1016/j.diagmicrobio.2008.03.006

subject

Has Abstract

pub_date

2008-08-01 00:00:00

pages

428-33

issue

4

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(08)00163-6

journal_volume

61

pub_type

杂志文章

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