Quantification and identification of polyomavirus DNA in blood and urine of renal transplant recipients.


:A cohort of 201 kidney transplant recipients (KTR) including 7 patients with evidence of renal function deterioration (as defined by creatinine levels >20% over baseline values) was analyzed for polyomavirus DNA in blood and urine samples by a new quantitative polymerase chain reaction method. Of 201 patients, 14 (6.9%) were positive for polyomavirus DNA in blood (median level, 500 copies per milliliter of blood) including all 7 patients with renal function deterioration. Polyomavirus DNA detection in blood for diagnosis of renal function deterioration in KTR showed a sensitivity of 100% and a specificity of 96%, whereas positive and negative predictive values were 50% and 100%, respectively. Diagnostic value of decoy cells detection and polyomavirus DNA quantification in urine samples was negligible.


Baldanti F,Fogazzi GB,Furione M,Saglimbeni L,Rovida F,Gatti M,Paolucci S,Tarantino A,Gerna G




Has Abstract


2007-03-01 00:00:00














  • Antimicrobial activity of cefepime tested against Bush group I beta-lactamase-producing strains resistant to ceftazidime. A multilaboratory national and international clinical isolate study.

    abstract::The potency of cefepime, a parenteral aminothiazolyl methoxyimino cephalosporin, was assessed against 256 ceftazidime-resistant Gram-negative bacilli from five medical centers in the United States. In addition, cefepime activity was compared with that of ciprofloxacin and imipenem against 506 ceftazidime-resistant Gra...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章,多中心研究


    authors: Jones RN,Marshall SA

    更新日期:1994-05-01 00:00:00

  • Colistin-resistant Enterobacteriaceae infections: clinical and molecular characterization and analysis of in vitro synergy.

    abstract::We described 27 polyclonal colistin-resistant Enterobacteriaceae (MIC 4-16 μg/mL) infections (12 pneumonia, 12 urinary tract infection (UTI), two Bacteremia, and one skin/soft tissue infection) in which 74% harbored KPC. The isolates were polyclonal, 6 STs were identified and the colistin resistance was due to chromos...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: de Maio Carrillho CM,Gaudereto JJ,Martins RC,de Castro Lima VA,de Oliveira LM,Urbano MR,Perozin JS,Levin AS,Costa SF

    更新日期:2017-03-01 00:00:00

  • Simple, rapid, and affordable point-of-care test for the serodiagnosis of typhoid fever.

    abstract::We developed a point-of-care test for the serodiagnosis of typhoid fever in the format of an immunochromatographic lateral flow assay. The flow assay for typhoid fever is based on the detection of Salmonella enterica serotype Typhi lipopolysaccharide-specific immunoglobulin M (IgM) antibodies. The assay was evaluated ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Pastoor R,Hatta M,Abdoel TH,Smits HL

    更新日期:2008-06-01 00:00:00

  • A rapid, standardized protein extraction method using adaptive focused acoustics for identification of mycobacteria by MALDI-ToF MS.

    abstract::Mycobacterial identification using MALDI-ToF MS (MALDI) has been hindered by inadequate extraction methods. Adaptive Focused Acoustics™ uses concentrated ultrasonic energy to achieve cellular disruption. Using this technology, we developed a rapid mycobacterial inactivation/protein extraction method for MALDI-based id...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Adams LL,Dionne K,Fisher S,Parrish N

    更新日期:2016-11-01 00:00:00

  • Clinical, immunological and microbiological predictors of poor outcome in Clostridium difficile infection.

    abstract:BACKGROUND:Clostridium difficile infection (CDI) causes increased morbidity and mortality. Clinical data cannot clearly predict poor CDI outcome. Data on the value of microbiological predictors is scarce. OBJECTIVE:To identify early predictors of poor outcome of CDI. METHODS:We prospectively included patients with CD...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Reigadas E,Alcalá L,Marín M,Martín A,Bouza E

    更新日期:2017-08-01 00:00:00

  • Application of a loop-mediated isothermal amplification method for the detection of pathogenic Leptospira.

    abstract::Leptospirosis is an emerging infectious disease, which is considered to be the most widespread zoonotic disease in the world. There are more than 230 known serovars in the genus Leptospira. A loop-mediated isothermal amplification (LAMP) assay for the rapid detection of pathogenic Leptospira spp. was developed and eva...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Lin X,Chen Y,Lu Y,Yan J,Yan J

    更新日期:2009-03-01 00:00:00

  • Development of a rapid assay methodology for antimicrobial susceptibility testing of Staphylococcus aureus.

    abstract::Development of a new phenotypic technique for rapid antimicrobial susceptibility testing (AST) of methicillin-resistant Staphylococcus aureus is presented. The new technique combines bacterial culturing and specific immunometric detection in a single separation-free process. The technique uses dry chemistry reagents a...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Koskinen JO,Stenholm T,Vaarno J,Soukka J,Meltola NJ,Soini AE

    更新日期:2008-11-01 00:00:00

  • SPM-1-producing Pseudomonas aeruginosa ST277 clone recovered from microbiota of migratory birds.

    abstract::The production of São Paulo metallo-β-lactamase (SPM-1) is the most common carbapenem resistance mechanism detected among multidrug-resistant Pseudomonas aeruginosa clinical isolates in Brazil. Dissemination of SPM-1-producing P. aeruginosa has been restricted to the nosocomial settings, with sporadic reports of envir...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Martins WMBS,Narciso AC,Cayô R,Santos SV,Fehlberg LCC,Ramos PL,da Cruz JB,Gales AC

    更新日期:2018-03-01 00:00:00

  • Anti-Mycoplasma pneumoniae secretory antibody in human breast milk.

    abstract::The secretory antibody responses to M. pneumoniae polypeptides were assessed among human breast milk samples. M. pneumoniae polypeptides were resolved with conventional SDS-PAGE, and antibody responses were determined by immunoblotting. Antibodies that contained secretory component were found to recognize antigens of ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Macadam S,Cimolai N

    更新日期:2002-07-01 00:00:00

  • Rapid identification of Branhamella catarrhalis. A comparison of five rapid methods.

    abstract::Five methods for the rapid identification and differentiation of Branhamella catarrhalis from other Neisseria species in 86 respiratory specimens were compared. These tests included the 4-methylumbelliferyl butyrate (MUB), API quadFerm, B.CAT.Confirm, Gonochek II, and the Tributyrin disc. All five tests reliably and a...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Louie M,Ongsansoy EG,Forward KR

    更新日期:1990-05-01 00:00:00

  • Case report: lymphogranuloma venereum proctitis-from rapid screening to molecular confirmation of a masked sexually transmitted disease.

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    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Markowicz M,Grilnberger E,Huber F,Leibl G,Abrahamian H,Gartner M,Huber M,Chott A,Reiter M,Stanek G

    更新日期:2013-08-01 00:00:00

  • A positive pneumococcal urinary antigen test promotes narrow spectrum antibiotic use in patients with non-invasive pneumococcal pneumonia.

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    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Banks R,Zappernick T,Wilson B,Perez F,Jump RLP

    更新日期:2020-02-01 00:00:00

  • Activity of doripenem with and without levofloxacin, amikacin, and colistin against Pseudomonas aeruginosa and Acinetobacter baumannii.

    abstract::At 24 h, sub-MIC doripenem and levofloxacin showed synergy against 21 of 25 Pseudomonas aeruginosa strains, sub-MIC doripenem and amikacin against 22 isolates, and sub-MIC doripenem and colistin against 19 isolates. Of 25 Acinetobacter baumannii strains, sub-MIC doripenem and levofloxacin showed synergy against 11 str...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Pankuch GA,Seifert H,Appelbaum PC

    更新日期:2010-06-01 00:00:00

  • Comparative in vitro activities of ampicillin, BMY 28142, and imipenem against Mycobacterium avium complex.

    abstract::The in vitro activity of ampicillin, BMY 28142, and imipenem was evaluated against 21 clinical isolates of Mycobacterium avium complex by both a broth and an agar dilution method. The MIC90 by broth dilution for ampicillin, BMY 28142, and imipenem was 16 micrograms/ml, 8 micrograms/ml, and greater than 32 micrograms/m...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Cynamon MH,Palmer GS,Sorg TB

    更新日期:1987-02-01 00:00:00

  • Prototheca zopfii isolated from a patient with olecranon bursitis.

    abstract::Prototheca zopfii was isolated from a patient with olecranon bursitis. Olecranon bursitis caused by Prototheca is a distinct clinical entity. Recognition of this infection was made by observing characteristic organisms in tissue and subsequent isolation of the organism. ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Naryshkin S,Frank I,Nachamkin I

    更新日期:1987-02-01 00:00:00

  • Gatifloxacin in community-based treatment of acute respiratory tract infections in the elderly.

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    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 临床试验,杂志文章,多中心研究


    authors: Nicholson SC,High KP,Gothelf S,Webb CD

    更新日期:2002-09-01 00:00:00

  • Real-world performance of a microarray-based rapid diagnostic for Gram-positive bloodstream infections and potential utility for antimicrobial stewardship.

    abstract::The Verigene Gram-positive blood culture assay (BC-GP) is a microarray-based rapid diagnostic test, which includes targets for 12 bacterial species and 3 resistance determinants. We prospectively compared the diagnostic accuracy of the BC-GP to routine microbiologic methods and evaluated the potential of the BC-GP for...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Aitken SL,Hemmige VS,Koo HL,Vuong NN,Lasco TM,Garey KW

    更新日期:2015-01-01 00:00:00

  • Disseminated "Haemophilus quentini" infection in a patient with multiple myeloma - a case report and review of the literature.

    abstract::We describe a case report of a 56-year-old male with undiagnosed multiple myeloma who had severe sepsis associated with pneumonia, meningitis, polyarthritis, and osteomyelitis related to invasive "Haemophilus quentini" infection. The genus was misidentified as H. influenzae by the common bacterial identification syste...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章,评审


    authors: Cohen R,Finn T,Babushkin F,Karalnik S,Paikin S,Adler A,Geffen Y,Rokney A,Ron M

    更新日期:2019-07-01 00:00:00

  • Vertical transmission of human immunodeficiency virus type 1. Prognostic value of IgA antibody to HIV-1 polypeptides during pregnancy.

    abstract::In a retrospective study of 31 pregnant women infected with human immunodeficiency virus type 1 (HIV-1), nine (29%) infants presented unequivocal signs of HIV-1 infection (persistent p24 antigenemia and/or positive virus isolation). All serum samples obtained from the others, during pregnancy and on delivery, were stu...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Re MC,Furlini G,Vignoli M,Ricchi E,Ramazzotti E,Bianchi S,Guerra B,Costigliola P,La Placa M

    更新日期:1992-08-01 00:00:00

  • Comparison of eight commercial enzyme immunoassays for the detection of Clostridium difficile from stool samples and effect of strain type.

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    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: René P,Frenette CP,Schiller I,Dendukuri N,Brassard P,Fenn S,Loo VG

    更新日期:2012-05-01 00:00:00

  • Characterization of Trypanosoma cruzi isolated from humans, vectors, and animal reservoirs following an outbreak of acute human Chagas disease in Santa Catarina State, Brazil.

    abstract::During March 2005, 24 cases of acute human Chagas disease were detected in Santa Catarina State, southern Brazil, all of them related to the ingestion of Trypanosoma cruzi-contaminated sugar cane juice. Following field studies allowed the isolation of 13 T. cruzi strains from humans, opossums (Didelphis aurita and Did...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Steindel M,Kramer Pacheco L,Scholl D,Soares M,de Moraes MH,Eger I,Kosmann C,Sincero TC,Stoco PH,Murta SM,de Carvalho-Pinto CJ,Grisard EC

    更新日期:2008-01-01 00:00:00

  • Extended spectrum beta-lactamase production and fluorquinolone resistance in pathogens associated with community acquired urinary tract infection.

    abstract::We have evaluated the susceptibility of 199 pathogens isolated in pure culture from consecutive urine samples submitted from the community. Rates of susceptibility for all organisms were ampicillin, 48%; amoxycillin/clavulanic acid, 88%; cephalothin, 57%; cefuroxime axetil, 74%; nalidixic acid, 85%; ciprofloxacin, 99%...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Cormican M,Morris D,Corbett-Feeeney G,Flynn J

    更新日期:1998-12-01 00:00:00

  • Evaluation of interpretive criteria of agar dilution and disk diffusion susceptibility tests for Neisseria gonorrhoeae.

    abstract::Using strains of Neisseria gonorrhoeae from Western New York, the National Committee for Clinical Laboratory Standards' (NCCLS) interpretive criteria for disk diffusion susceptibility testing was evaluated on chocolate-Mueller-Hinton agar (CMH) and GC agar. The reference method for comparison was the NCCLS agar diluti...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Altaie SS,Moore LS,Dryja D,Furness K

    更新日期:1994-03-01 00:00:00

  • In vitro activity of cefoperazone/sulbactam and other antimicrobials against anaerobic bacteria.

    abstract::Sulbactam inhibits the hydrolytic activity of several, clinically important beta-lactamases including those produced by anaerobic bacteria. This study was undertaken to determine the effect of sulbactam on the activity of cefoperazone against 250 anaerobic bacteria including 174 isolates belonging to the Bacteroides f...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: D'Amato RF,Hochstein L,Frankel H

    更新日期:1990-01-01 00:00:00

  • Salmonella prosthetic valve endocarditis.

    abstract::Four cases of Salmonella prosthetic valve endocarditis have been reported previously in the English medical literature (Fraser et al. 1967; Yamamoto et al., 1974; Shanson et al., 1977; Bassa et al., 1989). This report describes a fifth case in a 62-year-old woman who developed tricuspid valve endocarditis after an epi...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章,评审


    authors: Choo PW,Gantz NM,Anderson C,Maguire JH

    更新日期:1992-03-01 00:00:00

  • Cefmetazole and trospectomycin in vitro susceptibility testing interpretive criteria and quality control guidelines for Neisseria gonorrheae.

    abstract::Cefmetazole and trospectomycin were tested in a multilaboratory trial to establish Neisseria gonorrhoeae susceptibility testing criteria and quality control (QC) guidelines. Cefmetazole was active against the penicillinase-producing isolates and has an MIC90 of 16 micrograms/ml, the breakpoint MIC previously used for ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 指南,杂志文章


    authors: Jones RN,Erwin ME,Washington JA,Koontz FP,Murray PR,Gerlach EH

    更新日期:1992-09-01 00:00:00

  • Host and microbiologic factors associated with mortality in Taiwanese children with invasive pneumococcal diseases, 2001 to 2006.

    abstract::We concurrently evaluated host- and organism-related factors in determining the outcomes of 160 invasive pneumococcal diseases episodes in 158 Taiwanese children during 2001 and 2006. Most (138/160, 86.2%) episodes occurred at age less than 60 months, and an underlying condition was present in 35 (22.2%) cases. Common...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Chen CJ,Lin CL,Chen YC,Wang CW,Chiu CH,Lin TY,Huang YC

    更新日期:2009-02-01 00:00:00

  • Low intrathecal immune response of anti-EBNA-1 antibodies and EBV DNA from multiple sclerosis patients.

    abstract::Numerous studies have been carried out to determine whether an Epstein-Barr virus (EBV) infection can be considered a risk factor for multiple sclerosis (MS), following the evidence of an increase in IgG response to nuclear antigen-1 (EBNA-1) in both serum and cerebrospinal fluid (CSF) from MS patients. However, the p...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Villegas E,Santiago O,Carrillo JA,Sorlózano A,Guerrero M,Fernández O,Gutiérrez J

    更新日期:2011-05-01 00:00:00

  • Performance of the Gen-Probe amplified Mycobacterium tuberculosis direct test in a laboratory that infrequently isolates Mycobacterium tuberculosis.

    abstract::The performance of the Gen-Probe Mycobacterium tuberculosis direct (MTD) test was assessed in a laboratory whose specimens were derived from a population with a low prevalence (1.3%) of tuberculosis. A total of 339 specimens from 113 patients were included in the study. Nine of 10 MTD positive samples were culture pos...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Welch K,Brown G,Jonas V,Ferraro MJ

    更新日期:1995-07-01 00:00:00

  • Cross-reactivity of non-Aspergillus fungal species in the Aspergillus galactomannan enzyme immunoassay.

    abstract::The Aspergillus galactomannan enzyme-linked immunosorbant assay (EIA) has been demonstrated to facilitate rapid and sensitive detection of invasive aspergillosis. However, test specificity has not been fully evaluated in non-Aspergillus fungal species. Of 53 fungal isolates, cross-reactivity was observed with 5 non-As...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Cummings JR,Jamison GR,Boudreaux JW,Howles MJ,Walsh TJ,Hayden RT

    更新日期:2007-09-01 00:00:00