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Comparison of four PCR methods for efficient detection of Trypanosoma cruzi in routine diagnostics.

Abstract：

:Due to increased migration, Chagas disease has become an international health problem. Reliable diagnosis of chronically infected people is crucial for prevention of non-vectorial transmission as well as treatment. This study compared four distinct PCR methods for detection of Trypanosoma cruzi DNA for the use in well-equipped routine diagnostic laboratories. DNA was extracted of T. cruzi-positive and negative patients' blood samples and cultured T. cruzi, T. rangeli as well as Leishmania spp. One conventional and two real-time PCR methods targeting a repetitive Sat-DNA sequence as well as one conventional PCR method targeting the variable region of the kDNA minicircle were compared for sensitivity, intra- and interassay precision, limit of detection, specificity and cross-reactivity. Considering the performance, costs and ease of use, an algorithm for PCR-diagnosis of patients with a positive serology for T. cruzi antibodies was developed.

journal_name

Diagn Microbiol Infect Dis

journal_title

Diagnostic microbiology and infectious disease

authors

Seiringer P,Pritsch M,Flores-Chavez M,Marchisio E,Helfrich K,Mengele C,Hohnerlein S,Bretzel G,Löscher T,Hoelscher M,Berens-Riha N

doi

10.1016/j.diagmicrobio.2017.04.003

subject

Has Abstract

pub_date

2017-07-01 00:00:00

pages

225-232

issue

3

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(17)30114-1

journal_volume

88

pub_type

杂志文章

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