当前位置： SCI文献检索 > DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE期刊下所有文献 > Rapid identification of medically important Candida to species level by polymerase chain reaction and single-strand conformational polymorphism.

Rapid identification of medically important Candida to species level by polymerase chain reaction and single-strand conformational polymorphism.

Abstract：

:Invasive fungal disease has taken a great toll on immunocompromised patients. With the emergence of fluconazole and amphotericin B resistance, the rapid identification of fungi to species level is of clinical relevance in guiding appropriate antifungal therapy. Among these opportunistic fungi, Candida species are the most commonly encountered. We had developed a molecular method utilizing single-strand conformational polymorphism (SSCP) to delineate different patterns on a 260-bp amplicon from the 28S rRNA gene from six medically important Candida species. The SSCP banding patterns obtained from a total of 52 isolates were sufficiently unique to allow distinction between the species, thus indicated a high level of specificity. This method of PCR-SSCP can provide a simple and specific method for the rapid identification of medically important Candida to species level.

journal_name

Diagn Microbiol Infect Dis

journal_title

Diagnostic microbiology and infectious disease

authors

Hui M,Ip M,Chan PK,Chin ML,Cheng AF

doi

10.1016/s0732-8893(00)00171-1

subject

Has Abstract

pub_date

2000-10-01 00:00:00

pages

95-9

issue

2

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(00)00171-1

journal_volume

38

pub_type

杂志文章

相关文献

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE文献大全
  • A culture medium for screening 16S rRNA methylase-producing pan-aminoglycoside resistant Gram-negative bacteria.

    abstract：:The amikacin plus gentamicin-containing SuperAminoglycoside medium was developed for screening multiple-aminoglycoside resistance in Gram-negative bacteria (Enterobacteriaceae, Pseudomonas aeruginosa, and Acinetobacter baumannii). It was evaluated using aminoglycoside-susceptible (n=12) and aminoglycoside-resistant (n...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2018.01.026

    authors： Nordmann P,Mazé A,Culebras E,Dobias J,Jayol A,Poirel L

    更新日期：2018-06-01 00:00:00

  • Pleural effusion with negative culture: a challenge for pneumococcal diagnosis in children.

    abstract：BACKGROUND:Pneumococcal parapneumonic effusion seems to be increasing in children in the postvaccine era and is frequently associated with negative culture. Due to the low yield of culture, culture-independent tools are evaluated. METHODS:Culture-negative pleural fluid specimens from 38 children with parapneumonic eff...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2016.07.022

    authors： Perez VP,Caierão J,Fischer GB,Dias CA,d'Azevedo PA

    更新日期：2016-10-01 00:00:00

  • Differentiation of Enterobacteriaceae, Pseudomonas aeruginosa, and Bacteroides and Haemophilus species in gram-stained direct smears.

    abstract：:The accuracy of examination of the Gram-stained direct smear to classify presumptively Gram-negative rods into three morphotype groups, that is, (a) Enteric bacteria, (b) Pseudomonas, and (c) Bacteroides or Haemophilus, was evaluated. Randomly selected clinical strains (4-9) each of Escherichia coli, Klebsiella pneumo...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(91)90032-b

    authors： Bartlett RC,Mazens-Sullivan MF,Lerer TJ

    更新日期：1991-05-01 00:00:00

  • Characterization of novel Mycobacterium tuberculosis pncA gene mutations in clinical isolates from the Ukraine.

    abstract：:Multidrug-resistant (MDR) and extensively drug-resistant (XDR) Mycobacterium tuberculosis cases in the Ukraine are increasing. Pyrazinamide (PZA) is critically important for first- and second-line tuberculosis (TB) treatment regimes. However, PZA drug susceptibility testing is time consuming and technically challengin...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2018.10.018

    authors： Daum LT,Konstantynovska OS,Solodiankin OS,Poteiko PI,Bolotin VI,Rodriguez JD,Gerilovych AP,Chambers JP,Fischer GW

    更新日期：2019-04-01 00:00:00

  • Diagnostic performance of a new automated heterophile antibody test in adults and children.

    abstract：:Epstein-Barr virus serologic profiles were obtained from 618 patients using an automated platform, including the new BioPlex heterophile assay. When compared with 2 IgM viral capsid antigen (VCA) assays and a predicate heterophile assay, the BioPlex heterophile assay was more likely to be associated with serologic pat...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2008.02.016

    authors： Klutts JS,Wu AH,Smith A,Yen-Lieberman B,Gronowski AM

    更新日期：2008-07-01 00:00:00

  • Quantification and identification of polyomavirus DNA in blood and urine of renal transplant recipients.

    abstract：:A cohort of 201 kidney transplant recipients (KTR) including 7 patients with evidence of renal function deterioration (as defined by creatinine levels >20% over baseline values) was analyzed for polyomavirus DNA in blood and urine samples by a new quantitative polymerase chain reaction method. Of 201 patients, 14 (6.9...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2006.08.006

    authors： Baldanti F,Fogazzi GB,Furione M,Saglimbeni L,Rovida F,Gatti M,Paolucci S,Tarantino A,Gerna G

    更新日期：2007-03-01 00:00:00

  • Diagnosis of pneumococcal empyema using immunochromatographic test on pleural fluid and serotype distribution in Korean children.

    abstract：:To evaluate the diagnostic value of immunochromatographic test (ICT) on pleural fluid in diagnosing pneumococcal empyema in children and to determine pneumococcal serotypes, 62 exudative parapneumonic effusions from Korean children were tested with culture, ICT for S. pneumoniae, pneumococcal autolysin polymerase chai...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2011.09.025

    authors： Lee JH,Kim SH,Lee J,Choi EH,Lee HJ

    更新日期：2012-02-01 00:00:00

  • Oral ofloxacin for infections caused by bacteria resistant to oral antimicrobial agents.

    abstract：:We conducted an open trial of oral ofloxacin, 400 mg q 12 hr, in the treatment of infections caused by Pseudomonas aeruginosa and other bacteria resistant to traditional oral antibiotics. There were 53 evaluable subjects, 30 having infection of the skin/skin structure, 13 of the respiratory tract, six of bone, and fou...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 临床试验,杂志文章

    doi：10.1016/0732-8893(92)90085-8

    authors： Eron LJ,Gentry LO

    更新日期：1992-07-01 00:00:00

  • Detection of Pneumocystis carinii by DNA amplification in human immunodeficiency virus-positive patients.

    abstract：:The opportunistic pathogen Pneumocystis carinii (PC) is a frequent cause of a life-threatening pneumonia in human immunodeficiency virus (HIV)-infected individuals and in other immunocompromised hosts. Specimens obtained from 128 bronchoalveolar lavage (BAL) fluid samples from 123 HIV-positive patients with pulmonary ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 临床试验,杂志文章

    doi：10.1016/s0732-8893(97)00126-0

    authors： Savoia D,Millesimo M,Cassetta I,Forno B,Caramello P

    更新日期：1997-10-01 00:00:00

  • In vitro activity of lomefloxacin against multiply resistant Pseudomonas aeruginosa, Enterobacter cloacae, and Staphylococcus epidermidis.

    abstract：:Lomefloxacin is a promising new difluorinated quinolone with good antimicrobial activity and favorable pharmacokinetic properties. The in vitro activity of lomefloxacin was compared to other quinolones, expanded spectrum beta-lactams, and gentamicin. The lomefloxacin MIC90 against multiply resistant strains of P. aeru...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(89)90059-x

    authors： DiVincenzo CA,Shatzer KL,Venezio FR

    更新日期：1989-05-01 00:00:00

  • Pharmacokinetics of cefotaxime in healthy volunteers and patients.

    abstract：:Cefotaxime is a third-generation cephalosporin that has maintained good susceptibility pattern despite its extensive use. It is available for intravenous and intramuscular administration. Its pharmacokinetic property includes a small volume of distribution with low protein binding. Cefotaxime's half-life is approximat...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(95)00072-i

    authors： Patel KB,Nicolau DP,Nightingale CH,Quintiliani R

    更新日期：1995-05-01 00:00:00

  • Improvement of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry identification of difficult-to-identify bacteria and its impact in the workflow of a clinical microbiology laboratory.

    abstract：:This study evaluates matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) capability for the identification of difficult-to-identify microorganisms. A total of 150 bacterial isolates inconclusively identified with conventional phenotypic tests were further assessed by 16S rRNA se...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2014.01.021

    authors： Rodríguez-Sánchez B,Marín M,Sánchez-Carrillo C,Cercenado E,Ruiz A,Rodríguez-Créixems M,Bouza E

    更新日期：2014-05-01 00:00:00

  • Cultures of sternal wound and mediastinum taken at the end of heart surgery do not predict postsurgical mediastinitis.

    abstract：:The aim of the study was to assess of the role of intraoperative cultures taken at the end of major heart surgery (MHS) in the prediction of postoperative mediastinitis (PM) in patients undergoing MHS over a 6-month period in a tertiary university hospital. Just before wound closure, a sample of the sternal border was...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2006.06.012

    authors： Bouza E,Muñoz P,Alcalá L,Pérez MJ,Rincón C,Barrio JM,Pinto A

    更新日期：2006-12-01 00:00:00

  • Cefmetazole (CS-1170), a "new" cephamycin with a decade of clinical experience.

    abstract：:In vitro and in vivo study results were reviewed from cefmetazole, a "new" parenteral cephamycin. Cefmetazole's spectrum of activity was comparable to that of second-generation cephalosporins, which includes clinical coverage of many Enterobacteriaceae, Staphylococcus spp., streptococci, Haemophilus spp., pathogenic N...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章,评审

    doi：10.1016/0732-8893(89)90106-5

    authors： Jones RN

    更新日期：1989-09-01 00:00:00

  • Pharmacokinetics of polymyxin B1 in patients with multidrug-resistant Gram-negative bacterial infections.

    abstract：:Polymyxin B is increasingly used clinically for the treatment of multidrug-resistant Gram-negative infections, despite very limited understanding of its disposition in humans. The disposition of intravenous polymyxin B1 in 9 adult patients was characterized. Random blood samples (specifically timed in relation to the ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2007.08.008

    authors： Kwa AL,Lim TP,Low JG,Hou J,Kurup A,Prince RA,Tam VH

    更新日期：2008-02-01 00:00:00

  • Improved urine screening using a combination of leukocyte esterase and the Lumac system.

    abstract：:Three rapid urine screening tests, leukocyte esterase, nitrite, and the Lumac System for detection of bacterial ATP, were evaluated alone and in combination to determine their utility in screening urine specimens from male patients for bacteruria. The combination of leukocyte esterase and Lumac testing resulted in sig...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(85)90036-7

    authors： Pfaller MA,Scharnweber G,Stewart B,Koontz FP

    更新日期：1985-05-01 00:00:00

  • Value of blood culture time to positivity in identifying complicated nontyphoidal Salmonella bacteremia.

    abstract：:Few studies analyzed the association between blood culture time to positivity (TTP) and risk of complicated nontyphoidal Salmonella (NTS) bacteremia. We conducted a retrospective study of 206 patients (aged 60.4 ± 17.4 years) with NTS bacteremia during a 30-month period. Complicated NTS bacteremia was defined as the p...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2018.02.005

    authors： Chen SY,Weng TH,Tseng WP,Fu CM,Lin HW,Liao CH,Lee TF,Hsueh PR,Fang CC,Chen SY

    更新日期：2018-07-01 00:00:00

  • Treatment of non-life-threatening methicillin-resistant Staphylococcus aureus infections with alternative antimicrobial agents: a 2-year retrospective review.

    abstract：:The emergence of methicillin-resistant Staphylococcus aureus (MRSA) infections has created a need for additional antimicrobial options. Patients at the Minneapolis Veterans Affairs Medical Center, Minneapolis, MN, who received alternative (nonvancomycin, nonlinezolid) therapy for MRSA infections from January 2004 to D...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2008.10.001

    authors： Drekonja DM,Traynor LM,DeCarolis DD,Crossley KB,Johnson JR

    更新日期：2009-02-01 00:00:00

  • Comparison of vidas Clostridium difficile toxin-A assay and premier C. difficile toxin-A assay to cytotoxin-B tissue culture assay for the detection of toxins of C. difficile.

    abstract：:Damage to the intestinal mucosa by Clostridium difficile (CD) is toxin mediated. Two enzyme immunoassays (EIAs) for toxin-A detection, the automated Vitek immunodiagnostic assay system CDA (Vidas CDA), and the Premier toxin A (Premier) were tested for their ability to detect toxin A in 301 stool samples and compared w...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(93)90062-c

    authors： Knapp CC,Sandin RL,Hall GS,Ludwig MD,Rutherford I,Washington JA

    更新日期：1993-07-01 00:00:00

  • Antifungal susceptibility of 1000 Candida bloodstream isolates to 5 antifungal drugs: results of a multicenter study conducted in São Paulo, Brazil, 1995-2003.

    abstract：:We evaluated all Candida sp. bloodstream isolates obtained from patients admitted to 4 tertiary care hospitals between 1995 and 2003 in the city of São Paulo, Brazil. Susceptibility to amphotericin B, 5-fluorocytosine, fluconazole (FCZ), itraconazole (ITZ), and voriconazole (VCZ) was determined using the Clinical Labo...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章,多中心研究

    doi：10.1016/j.diagmicrobio.2006.10.011

    authors： da Matta DA,de Almeida LP,Machado AM,Azevedo AC,Kusano EJ,Travassos NF,Salomão R,Colombo AL

    更新日期：2007-04-01 00:00:00

  • Distribution of capsular and surface polysaccharide serotypes of Staphylococcus aureus.

    abstract：:Because of its ability to cause serious and fatal infections, Staphylococcus aureus remains one of the most feared microorganisms. Methicillin-resistant S. aureus (MRSA) has long been a common pathogen in healthcare facilities, but within the past decade, it has emerged as a problematic pathogen in the community setti...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2007.01.016

    authors： von Eiff C,Taylor KL,Mellmann A,Fattom AI,Friedrich AW,Peters G,Becker K

    更新日期：2007-07-01 00:00:00

  • Diagnostic value of F-18 FDG PET/CT in patients with spondylodiscitis: Is dual time point imaging time worthy?

    abstract：PURPOSE:In this retrospective study, we aimed to investigate the value of FDG-PET/CT in the diagnosis of spondylodiscitis (SD), the significance of dual time point imaging (DTPI) for SD diagnosis and the worth of SUVmax data for distinguishing tuberculous vs. non-tuberculous SD. MATERIALS AND METHODS:32 patients with ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2016.03.021

    authors： Gunes BY,Onsel C,Sonmezoglu K,Ozaras R,Halac M,Tabak F,Sager S,Mete B,Sayman H,Kanmaz B

    更新日期：2016-07-01 00:00:00

  • SPM-1-producing Pseudomonas aeruginosa ST277 clone recovered from microbiota of migratory birds.

    abstract：:The production of São Paulo metallo-β-lactamase (SPM-1) is the most common carbapenem resistance mechanism detected among multidrug-resistant Pseudomonas aeruginosa clinical isolates in Brazil. Dissemination of SPM-1-producing P. aeruginosa has been restricted to the nosocomial settings, with sporadic reports of envir...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2017.11.003

    authors： Martins WMBS,Narciso AC,Cayô R,Santos SV,Fehlberg LCC,Ramos PL,da Cruz JB,Gales AC

    更新日期：2018-03-01 00:00:00

  • Evaluation of commercial serologic test reagents for immunoidentification of medically important aspergilli.

    abstract：:We evaluated commercial serodiagnostic test reagents from Greer Laboratories (GL), Lenoir, NC; Immuno-mycologics, Inc. (IMI), Norman, OK; and Scott Laboratories (SL), Fiskville, RI; for their ability to detect Aspergillus spp. exoantigens and group them in their proper series. We detected 87 culture extracts from code...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(87)90170-2

    authors： Sekhon AS,Standard PG,Kaufman L,Garg AK

    更新日期：1987-11-01 00:00:00

  • Leukocytoclastic vasculitis complicating Klebsiella pneumoniae bacteremia.

    abstract：:A 79-year old woman, with a history of hypertension, presented with clinical features of congestive heart failure, fever, a purpuric rash, and left lower quadrant abdominal tenderness. Contrast computed tomography scan of the abdomen showed features of acute diverticulitis, and blood culture was subsequently positive ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/s0732-8893(00)00151-6

    authors： Lum PN,Woo PC,Wong SS,Yuen K

    更新日期：2000-08-01 00:00:00

  • Isolation of cholesterol-dependent Candida glabrata from clinical specimens.

    abstract：:In the past few years, we have detected in the United Kingdom and in the United States several isolates of Candida glabrata that grew poorly unless bile was available. Cholesterol, a component of bile, stimulated equivalent growth of the bile-dependent isolates. The bile-dependent C. glabrata isolates appeared resista...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2004.12.006

    authors： Hazen KC,Stei J,Darracott C,Breathnach A,May J,Howell SA

    更新日期：2005-05-01 00:00:00

  • Application of recombinant fimbrial protein for the specific detection of Salmonella enteritidis infection in poultry.

    abstract：:A number of disease outbreaks of Salmonella enterica serotype enteritidis (SE) in humans have been traced to the consumption of SE-contaminated egg and egg products. A rapid, specific, and inexpensive method of detecting SE infection in poultry is necessary to reduce human outbreaks. We evaluated rSEF14 fimbrial antig...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/s0732-8893(98)00091-1

    authors： Rajashekara G,Munir S,Lamichhane CM,Back A,Kapur V,Halvorson DA,Nagaraja KV

    更新日期：1998-11-01 00:00:00

  • Comparison of five different susceptibility test methods for detecting antimicrobial agent resistance among Haemophilus influenzae isolates.

    abstract：:The detection of antimicrobial agent resistance among ninety-eight Haemophilus influenzae isolates was assessed by six different antibiotic test methods: agar dilution on Mueller-Hinton agar supplemented with 5% lysed horse blood (MH-LHB), E-test using both Haemophilus test medium (HTM) agar and chocolate Mueller-Hint...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(96)00026-0

    authors： Giger O,Mortensen JE,Clark RB,Evangelista A

    更新日期：1996-03-01 00:00:00

  • The effect of fecal turbidity on norovirus detection by reverse transcriptase polymerase chain reaction.

    abstract：:A 10-min fecal preparation results in greater specimen turbidity than a 45-min protocol, but reverse transcriptase polymerase chain reaction (RT-PCR) norovirus test sensitivity is essentially the same. Feces processed so that particle size does not exceed approximately 560 nm do not display greater norovirus RT-PCR in...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2008.08.006

    authors： Witlox KJ,Karapanagiotidis T,Bruggink LD,Marshall JA

    更新日期：2010-02-01 00:00:00

  • Screening the enteroaggregative Escherichia coli activity and detection of the aggA, aafA, and astA genes with novel PCR primers for the Escherichia coli isolates from diarrhea cases in Taiwan.

    abstract：:Enteroaggregative Escherichia coli (EAggEC) are emerging enteropathogens associated with human diarrhea diseases and food poisoning cases. They show distinctive aggregative pattern of adherence to cultured human epithelial cells. However, EAggEC strains are diverse and not all of them have the aggregative adherence fi...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/s0732-8893(03)00043-9

    authors： Tsai CC,Chen SY,Tsen HY

    更新日期：2003-07-01 00:00:00