Abstract：
:The association of [3H] [D-Ala2, D-Leu5] enkephalin ([3H]DADLE]) with mouse neuroblastoma cells (N4TG1) was investigated. Under identical conditions the time course, dose response curve and temperature dependence for ligand uptake were similar to those for ligand-induced receptor loss (down regulation). Uptake of [3H]DADLE was inhibited by opiate ligands as well as by the metabolic inhibitors sodium azide and 2,4 dinitrophenol. Comparison of the effects of these inhibitors on receptor binding, ligand uptake and receptor loss indicated that these cells accumulate [3H]DADLE in excess of their surface receptor number. The data suggest that receptor recycling occurs and that ligand is internalized via receptor mediated endocytosis.
journal_name
Life Scijournal_title
Life sciencesauthors
Blanchard SG,Chang KJ,Cuatrecasas Pdoi
10.1016/0024-3205(82)90369-1subject
Has Abstractpub_date
1982-09-20 00:00:00pages
1311-4issue
12-13eissn
0024-3205issn
1879-0631pii
0024-3205(82)90369-1journal_volume
31pub_type
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