Abstract：
:Bone tissue is dependent on an efficient blood supply to ensure delivery of nutrients and oxygen. One method to acquire a vascular-engineered bone tissue could be the use of an angiogenic gene-activated scaffold. In the current study, porous chitosan/hydroxyapatite (C/HA) scaffolds were fabricated via freeze-drying with desired pore size, and then combined with the adenoviral vector encoding vascular endothelial growth factor and green fluorescence protein (Ad-VEGF). Human osteoblasts were cultured and seeded on characterized scaffolds. The attachment, proliferation, and differentiation of cells on gene-activated and unactivated C/HA scaffolds were evaluated in vitro and in vivo by histo- and immunohistochemistry. Findings confirmed that human osteoblasts cultured on gene-activated C/HA scaffold secreted vascular endothelial growth factor, besides maintaining its characteristic phenotype with specific extracellular matrix production. In vivo experiments indicated that scaffolds were tissue biocompatible, and that gene-activated scaffold provided a suitable environment for neovessel formation by recruiting host endothelial cells into the newly forming ectopic bone-like tissue. This study revealed that the Ad-VEGF-activated C/HA composite scaffold has potential for vascular bone regeneration applications.
journal_name
J Biomater Appljournal_title
Journal of biomaterials applicationsauthors
Koç A,Finkenzeller G,Elçin AE,Stark GB,Elçin YMdoi
10.1177/0885328214544769subject
Has Abstractpub_date
2014-11-01 00:00:00pages
748-60issue
5eissn
0885-3282issn
1530-8022pii
0885328214544769journal_volume
29pub_type
杂志文章abstract：:Antenna coupling microwave plasma enables a highly oxidative treatment of the outmost surface of polypropylene (PP) nonwoven fabric within a short time period. Subsequently, grafting copolymerization with acrylic acid (AAc) makes the plasma-treated fabric durably hydrophilic and excellent in water absorbency. With hig...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1106/088532802030478
更新日期：2002-10-01 00:00:00
abstract：:Calcium polyphosphate is a biodegradable bone substitute. It remains a challenge to prepare porous calcium polyphosphate with desired gradient porous structures. In this study, a modified one-step gravity sintering method was used to prepare calcium polyphosphate scaffolds with desired-gradient-pore-size distribution....
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328215608335
更新日期：2016-02-01 00:00:00
abstract：:The manufacture of a polyester vascular prosthesis in the southern hemisphere is a new development in the global dissemination of this maturing technology. Hence the recent introduction of the Barone Microvelour arterial graft from Argentina has highlighted the need for a comparative in vitro and in vivo study to comp...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/088532828800300302
更新日期：1989-01-01 00:00:00
abstract：:In this study, a phosphorylation treatment of porous anodic alumina (PAA) was performed by wet impregnation in phosphoric acid and a subsequent heat treatment. The PAA and phosphorylated PAA specimens were analyzed using a field emission scanning electron microscope, an energy-dispersive X-ray spectrometer, and Fourie...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328214526765
更新日期：2014-09-01 00:00:00
abstract：:Poor blood circulation makes it difficult for antitubercular drugs to achieve effective bactericidal concentration at tuberculose focus. The residual Mycobacterium tuberculosis around surgical wound would multiply, resulting in nonunion or sinus formation. Carbon nanotubes have strong tissue penetration and can cross ...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328218814988
更新日期：2019-02-01 00:00:00
abstract：:The animal immune response against Galα1,3-Galβ1-4GlcNAc-R(α-Gal) epitopes gives an important cause for the failure of glutaraldehyde(GA)-fixed cardiac xenografts. This study aimed to assess the in vivo effect of our novel combined anticalcification treatment, which includes immunologic modification, using α1,3-galact...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328214552710
更新日期：2015-02-01 00:00:00
abstract：:The process of molecular self-assembly plays a crucial role in formulation of polymeric nanoparticulated drug delivery carriers as it creates the possibility for enhanced drug encapsulation and carrier surface engineering. This study aimed to develop a novel self-assembled polymeric micelles for targeted delivery in t...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328214549612
更新日期：2015-01-01 00:00:00
abstract：:The carcinogenic potential of asbestos has led to the development of non-asbestos materials for use as casting ring liners. Cellulose paper are replacing asbestos paper. The brass die and crown were made to produce full crowns. The standardized wax pattern was replicated twenty-nine times. Two grooves were marked on t...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/088532820001400406
更新日期：2000-04-01 00:00:00
abstract：:Single nanofibers with chemical and functional properties consistent with artery extracellular matrix nanofibers were produced by electrospinning. Using weight ratios to mimic artery extracellular matrix, five materials were tested: (1) Collagen type I, (2) Collagen type I + Collagen type III, (3) Collagen type I + po...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328213477893
更新日期：2014-01-01 00:00:00
abstract：:We assess the effects of chemical processing, ethylene oxide sterilization, and threading on bone surface and mechanical properties of bovine undecalcified bone screws. In addition, we evaluate the possibility of manufacturing bone screws with predefined dimensions. Scanning electronic microscopic images show that che...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328208092110
更新日期：2009-03-01 00:00:00
abstract：:To develop biocompatible composite microspheres for novel hemostatic use, we designed and prepared a novel biomaterial, composite microspheres consisting of carboxymethyl chitosan, sodium alginate, and collagen (CSCM). The ultra-structure of CSCM was investigated by scanning electron microscopy assay. In hemostatic fu...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328215618354
更新日期：2016-02-01 00:00:00
abstract：:L-Tyrosine polyurethanes (LTUs) have been synthesized by structural modification of the poly (amino acid) backbone to circumvent the problems associated with the processing of poly (amino acids) arising from their high crystallinity, insolubility in common organic solvents, and high glass-transition and melting temper...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328211432325
更新日期：2013-05-01 00:00:00
abstract：:The incidence of fractures increases with age. This is partly due to extraosseous factors and partly to the increased fragility of the bone material itself. Ageing adversely affects the "quality" of human bone material, its elastic and ultimate properties. The hypothesis here is that these effects are caused by factor...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1106/5JUJ-TFJ3-JVVA-3RJ0
更新日期：2001-01-01 00:00:00
abstract：:Polysulfone (PSU) composite films filled with Beta-dicalcium silicate (Beta-Ca(2)SiO(4)) particles are prepared by the solvent casting-evaporation method. The surface morphologies and mechanical properties of the films are determined. The bioactivity of the composite films is evaluated by soaking them in simulated bod...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328206054712
更新日期：2006-04-01 00:00:00
abstract：:The setup of colloidal hybrid nanosystems based on biomimetic calcium phosphate apatites doped with europium ions has recently raised great interest in the pharmacological community, especially due to their bio-inspired character. This is especially relevant in relation with medical imaging for cancer diagnosis. Quest...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328212473510
更新日期：2014-01-01 00:00:00
abstract：:In this study, the effects of sodium carbonate (Na(2)CO(3) ) solution with different concentrations (10, 15, 20, and 25 wt%) as liquid phase on the setting time and compressive strength of tricalcium silicate bone cements are investigated. The in vitro bioactivity and degradability of the resultant Ca(3)SiO(5)-Na(2)CO...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328208088714
更新日期：2008-11-01 00:00:00
abstract：PURPOSE:To produce biodurable small diameter microporous vascular grafts with self-sealing properties for vascular access, for peripheral vascular and potentially for coronary artery bypass. The prosthesis should retain compliance and pulsatile flow in situ using a unique modus operandi permitting wall compression whic...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/088532829501000207
更新日期：1995-10-01 00:00:00
abstract：:The development of an effective wound dressing with the ability to induce skin wound healing is a great challenge in medicine. Nanofibers are highly attractive for wound dressing preparation due to their properties such as hemostasis induction, good absorption of wound exudates, and facilitation of cell growth. Chitos...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328217739562
更新日期：2018-01-01 00:00:00
abstract：:Herb borneol is usually used in clinics for the treatment of central nervous system illness, for its ability of blood-brain barrier permeability, although its poor water solubility and poor bioavailability limit its clinical application to some degree. In this study, we developed a novel nanoparticle combining the ben...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328218799551
更新日期：2018-10-01 00:00:00
abstract：:In this work, we report brushite-based calcium phosphate cement (CPC) system to enhance the in vivo biodegradation and tissue in-growth by incorporation of micro-channeled hydroxyapatite (HAp) granule and silicon and sodium addition in calcium phosphate precursor powder. Sodium- and silicon-rich calcium phosphate powd...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328215601938
更新日期：2016-01-01 00:00:00
abstract：:An electronic sensor system for urinary bladder pressure monitoring requires an imbedding into a biocompatible, flexible, and liquid-impermeable material. Poly(dimethylsiloxane) (PDMS) was selected in the present set-up as packaging material because it fulfills the abovementioned requirements. However, the surface of ...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328211422119
更新日期：2013-02-01 00:00:00
abstract：:Using a hydraulic stress simulator, the mechanical behavior of the porcine pericardium used in the construction of cardiac valve leaflets was characterized following the same procedure employed with calf pericardium in Part 1 of this study. One hundred fifty pairs of tissue samples were subjected to tensile testing to...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1106/3JXM-UTPN-PXTG-72DT
更新日期：2001-07-01 00:00:00
abstract：:Polymer biocomposites based on segmented poly(ester urethane) and extracellular matrix components have been prepared for the development of tissue engineering applications with improved biological characteristics of the materials in contact with blood and tissues for long periods. Thermal, dynamical, and dielectrical ...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328210394468
更新日期：2012-08-01 00:00:00
abstract：:Nanodrug delivery systems have been widely researched to achieve efficient antitumor drug delivery. However, the controlled drug delivery at tumor cells remains the main challenge for antitumor therapy. Herein, a pH and reduction-responsive nanocarrier based on green tea polyphenols was employed as a smart excipient f...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328220924539
更新日期：2020-08-01 00:00:00
abstract：:The in vitro effect of platelet-rich plasma (PRP) on cell loading, proliferation, and osteogenic differentiation of human mesenchymal stem cells (MSC) is assessed on distinct resorbable and synthetic calcium phosphate scaffolds. A high specific surface area scaffold composed of calcium-deficient hydroxyapatite (CDHA; ...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328207088269
更新日期：2008-09-01 00:00:00
abstract：:This study reports the properties of tableted microparticles based on high-amylose corn starch (HACS)-pectin blend polymers as the controlled release system for diclofenac sodium (DS). HACS-pectin blend microparticles are prepared through a modified process by the spray drying technique, which is a widely used microen...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328206056771
更新日期：2007-01-01 00:00:00
abstract：:Calcium phosphate fillers have been shown to increase cement osteoconductivity, but have caused drawbacks in cement properties. Hydroxyapatite and Brushite were introduced in an acrylic two-solution cement at varying concentrations. Novel composite bone cements were developed and characterized using rheology, injectab...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328214544770
更新日期：2014-11-01 00:00:00
abstract：:In order to induce esophageal muscle cells' orientation, the silicon wafer with prototype 1 and prototype 2 was designed. Prototype 1 has micro-channels of 200 µm width and 30 µm depth with 30 µm wide wall as the interval. Prototype 2 has channels of 100 µm width and 30 µm depth with a discontinuous wall which has 30 ...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328215627585
更新日期：2016-04-01 00:00:00
abstract：:The aim of this study was to evaluate the possibility of metal complex formation between sodium-phosphorylated chitosan (PCTS) and ZnO. The polymer-metal complex formation was investigated in terms of thermal degradation. The structure deduction of the PCTS/ZnO complex was investigated by means of Fourier transform in...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328211408371
更新日期：2012-11-01 00:00:00
abstract：:Devices such as contact lenses and collagen shields have been used to improve the antibiotic bioavailability of eye drops formulations in the treatment of ulcerative keratitis. Nevertheless, these devices are not sustained drug delivery systems, and a combination with eye drops is necessary. In animal patients, it req...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328220931733
更新日期：2020-09-01 00:00:00
