Abstract:
:We have determined the high resolution crystal structure of the A domain from the alpha chain of integrin CR3. The domain adopts a classic alpha/beta "Rossmann" fold and contains an unusual Mg2+ coordination site at its surface. One of the coordinating ligands is the glutamate side chain from another A domain molecule. We suggest that this site represents a general metal ion-dependent adhesion site (MIDAS) for binding protein ligands. We further propose that the beta subunits of integrins contain a MIDAS motif within a modified A domain. Our crystal structure will allow reliable models to be built for other members of the A domain superfamily and should facilitate development of novel adhesion modulatory drugs.
journal_name
Celljournal_title
Cellauthors
Lee JO,Rieu P,Arnaout MA,Liddington Rdoi
10.1016/0092-8674(95)90517-0subject
Has Abstractpub_date
1995-02-24 00:00:00pages
631-8issue
4eissn
0092-8674issn
1097-4172pii
0092-8674(95)90517-0journal_volume
80pub_type
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