Abstract：
UNLABELLED:Isolation of meiotic arrested oocytes from mammalian ovaries involves a resumption of the meiosis. Studying the effect of FSH on this process it was found that doses up to of 0.1 IU/ml did not maintain the meiotic arrest in hamster oocytes in pre-antral follicles during static incubation conditions. However, when the FSH medium was replaced at 120, 60 and 30 min the resumption of the meiosis gradually decreased. Using a replacement frequency of 30 min a complete log dose-curve was constructed (ED50: 0.5 mU/ml; minimum effective dose: 0.1 mU/ml). The lack of a FSH response during static incubation was not caused by exhaustion of its bioactivity: mixing of the FSH containing medium, obtained from a 4 hours' static incubation, resulted in an inhibition similar to the inhibition that occurred during the replacement procedure. IN CONCLUSION:FSH is able to maintain the meiotic arrest in pre-antral follicle enclosed oocytes at low doses only when the medium is in circulation.
journal_name
Life Scijournal_title
Life sciencesauthors
Goverde HJ,van de Venne WPdoi
10.1016/0024-3205(94)90045-0subject
Has Abstractpub_date
1994-01-01 00:00:00pages
885-91issue
11eissn
0024-3205issn
1879-0631pii
0024-3205(94)90045-0journal_volume
55pub_type
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