Genetic structure of Trypanosoma cruzi populations from Argentina estimated from enzyme polymorphism.

Abstract:

:Isolates of Trypanosoma cruzi from human patients, domestic and sylvatic animals and vector insects were obtained in different areas of Argentina. Electrophoretic patterns of enzymes from extracts of 95 isolates were analysed. On the basis of zymograms providing information on 10 loci, 12 zymodemes are described according to their genotypes. Data presented show fixed heterozygosity, absence of segregation of genotypes, significant departures from Hardy-Weinberg equilibrium, and over-represented genotypes. This evidence supports the hypothesis that sexual reproduction is very restricted or absent in this parasite. The proportion of polymorphic loci is 80%. The expected mean heterozygosity per locus (He) is 0.43, while the observed value (Ho) is 0.24. Differences between these values may be explained by accepting a basically clonal structure for T. cruzi. The data matrix of 12 zymodemes using 28 characters was analysed using a Wagner parsimony algorithm. Two equally most parsimonious unrooted trees were generated; both have 39 steps. The results show clusters clearly separated according to the geographical origin of the stocks. There are some indications of some correlations between genetic composition of the parasite and the clinical picture of the infection in human patients.

journal_name

Parasitology

journal_title

Parasitology

authors

de Luca D'oro GM,Gardenal CN,Perret B,Crisci JV,Montamat EE

doi

10.1017/s0031182000067755

subject

Has Abstract

pub_date

1993-11-01 00:00:00

pages

405-10

eissn

0031-1820

issn

1469-8161

journal_volume

107 ( Pt 4)

pub_type

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