Abstract:
:An M13 phage random 12-mers peptide library was used to screen cathepsin L mimotopes of Fasciola hepatica and to evaluate their immunogenicity in sheep. Seven clones showed positive reactivity to a rabbit anti-cathepsin L1/L2 antiserum in ELISA, and their amino acid sequences deduced by DNA sequencing were tentatively mapped on the protein. Twenty sheep were randomly allocated into 4 groups of 5 animals each, for immunization with 1x10(14) phage particles of clones 1, 20, a mixture of 7 clones and PBS, without adjuvant at the beginning, and 4 weeks later. All groups were challenged with 300 metacercariae at week 6 and slaughtered 16 weeks later. The mean worm burdens after challenge were reduced by 47.61% and 33.91% in sheep vaccinated with clones 1 and 20, respectively; no effect was observed in animals inoculated with the clone mixture. Also, a significant reduction in worm size and burden was observed for those sheep immunized with clone 1. Animals receiving clone 20, showed a significant reduction in egg output. Immunization induced a reduction of egg viability ranging from 58.92 to 82.11%. Furthermore, vaccinated animals produced clone-specific antibodies which were boosted after challenge with metacercariae of F. hepatica.
journal_name
Parasitologyjournal_title
Parasitologyauthors
Villa-Mancera A,Quiroz-Romero H,Correa D,Ibarra F,Reyes-Pérez M,Reyes-Vivas H,López-Velázquez G,Gazarian K,Gazarian T,Alonso RAdoi
10.1017/S003118200800471Xsubject
Has Abstractpub_date
2008-10-01 00:00:00pages
1437-45issue
12eissn
0031-1820issn
1469-8161pii
S003118200800471Xjournal_volume
135pub_type
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