In vitro titration of Theileria parva tick derived stabilates.

Abstract:

:Immunization against the protozoan Theileria parva by infection and treatment has proved to be very efficient for the control of East Coast fever, an acute and often-fatal lymphoproliferative tick-borne disease of cattle in Eastern, Central and Southern Africa. The immunizing dose of live T. parva sporozoites used in this method is usually determined by in vivo titration. An alternative in vitro method of quantification of sporozoites in whole tick-derived stabilates is proposed. The method consists of incubating serially diluted T. parva stabilates with bovine peripheral blood lymphocytes, the host cell that is infected naturally. Allowing the cultures to incubate undisturbed for the full cultivation period (10 days) reduced the variability among replicate titrations. Fungal contaminations were avoided by centrifuging stabilates at 400 g prior to the incubation, which did not precipitate sporozoites significantly. Fungistatics, Nystatin and Flucytosine, did not appear to interfere with the in vitro development of T. parva but their effect on fungal growth was limited. In vitro titration data were compared to in vivo infection data for 2 stabilates. In vitro titration of T. parva sporozoites should allow more ethical and efficient research on the preparation and storage of T. parva tick-derived stabilates.

journal_name

Parasitology

journal_title

Parasitology

authors

Marcotty T,Speybroeck N,Berkvens D,Chaka G,Besa R,Madder M,Dolan T,Losson B,Brandt J

doi

10.1017/s0031182003004323

subject

Has Abstract

pub_date

2004-02-01 00:00:00

pages

131-7

issue

Pt 2

eissn

0031-1820

issn

1469-8161

journal_volume

128

pub_type

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