Abstract:
:Evidences in the central nervous system are in favor that adenosine under basal conditions is released by a direct excitation-secretion modality. However, till now, there is no direct evidence that adenosine is contained in synaptic vesicles. Eight synaptic vesicle fractions were recovered on a discontinuous sucrose gradient after ultracentrifugation of the crude synaptosomal fraction (pellet P2) of rat brain. The adenosine content in each fraction was measured by high-performance liquid chromatography coupled to a fluorescence detector (minimum sensitivity 10 femtomoles). The immunoblot analysis, to detect synaptophysin, a molecular marker for the vesicle membrane, showed that fractions from 3 to 8 were rich in synaptophysin. The sum of adenosine found in fractions 3-8 was (mean ± SEM, n = 4) 3325.6 ± 94.6 pmol/mg of tissue protein. We proved that adenosine measured in synaptic vesicle fractions was not contaminated by cytosolic adenosine, as adenosine exogenously added to the P2 preferentially distributed in fractions 1 and 2 that are synaptophysin-free and did not contaminate the vesicle pellet P3. Data provide direct demonstration that adenosine is present in rat brain synaptic vesicle fractions. This information is consistent with the notion that adenosine is stored in synaptic vesicles and is released under normoxic physiological conditions by an excitation-secretion mechanism typical of neuronal cells.
journal_name
Neuroreportjournal_title
Neuroreportauthors
Corti F,Cellai L,Melani A,Donati C,Bruni P,Pedata Fdoi
10.1097/WNR.0000000000000033subject
Has Abstractpub_date
2013-12-04 00:00:00pages
982-7issue
17eissn
0959-4965issn
1473-558Xjournal_volume
24pub_type
杂志文章相关文献
NEUROREPORT文献大全abstract::The increase in neural activity in cat visual cortex associated with eye movements has been thought to reflect a replica of the motor command signal. We examined the timing of the saccade-related increase in neural activity in cat areas 17 and 18 in relation to saccade onset and offset. The increase in activity was te...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-200006050-00013
更新日期:2000-06-05 00:00:00
abstract::5-[125I]iodo-3-(2(S)-azetidinylmethoxy)pyridine ([125I]5-I-A-85380) was evaluated in the mouse as a potential in vivo imaging ligand for central nicotinic acetylcholine receptors (nAChRs). After i.v. administration of [125I]5-I-A-85380, peak brain levels of radioactivity were measured within 1 h and declined slowly ov...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-199807130-00030
更新日期:1998-07-13 00:00:00
abstract::To study the number cognition process, we recorded event-related potentials (ERPs) in 14 subjects when they were indicating whether a number pair was the same or not. The two numbers given in sequence were either the same (condition 1) or different (condition 2). After 270 ms following the onset of the second stimulus...
journal_title:Neuroreport
pub_type: 临床试验,杂志文章
doi:10.1097/00001756-200004270-00010
更新日期:2000-04-27 00:00:00
abstract::It is unclear whether the neural algorithm that underlies stereoscopic vision in birds incorporates both low level (camouflage breaking) and high level (depth ordering) comparisons of information available to each of the eyes. Both visual functions were successfully tested by examining transitive inference performance...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-199804200-00050
更新日期:1998-04-20 00:00:00
abstract::Using a guessing paradigm we measured visual sensitivity in the blind and normal half-fields of four cerebrally hemidecorticated patients. In the blind field, sensitivity was reduced by approximately 3 long units. Stimuli which produced significant detection also evoked conscious sensations of light and colour. Contro...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-199608120-00027
更新日期:1996-08-12 00:00:00
abstract::Extrasynaptic transmission is mediated by the diffusion of transmitters, through the extracellular space (ECS) to receptors on neurons and glia. The three-dimensional diffusion of tetramethylammonium (mol. wt 74.1 kDa) was investigated in the isolated rat spinal cord at postnatal days 4-20. The diffusion parameters of...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-199711100-00022
更新日期:1997-11-10 00:00:00
abstract::Spontaneous saccades of both eyes were recorded in head-restrained pigeons placed in 6 different visual conditions (darkness and biocular, uniocular, frontal or lateral viewing). Most saccades (95%) were biocular and directed forward (around the beak axis) and backward (around the horizontal line). In the dark, the pr...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-199201000-00004
更新日期:1992-01-01 00:00:00
abstract::The rat suprachiasmatic (SCN) contains a dense plexus of low-affinity p75 neurotrophin receptor (p75NTR)-immunoreactivity. In some SCN neurons, p75NTR is co-localized with vasoactive intestinal peptide (VIP). The present study examines the effect of third ventricle administration of 192 IgG-saporin immunotoxin on p75N...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-199810050-00012
更新日期:1998-10-05 00:00:00
abstract::Prepulse inhibition (PPI) of the acoustic startle reflex is a measure of sensorimotor gating which occurs in both rodents and humans. PPI is deficient in severe neuropsychiatric disorders such as schizophrenia. We investigated PPI in 10 adult monkeys (Cebus apella). Stimuli were 115 dB white noise startle pulses, eith...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-200101220-00031
更新日期:2001-01-22 00:00:00
abstract::This study examined 'prepulse inhibition' in the context of an auditory two-tone discrimination task performed by 15 healthy subjects. In order to distinguish between masking or excitatory information processes, weak acoustic pulses immediately preceded or succeeded a target or non-target tone in the discrimination. B...
journal_title:Neuroreport
pub_type: 临床试验,杂志文章
doi:10.1097/00001756-199601310-00065
更新日期:1996-01-31 00:00:00
abstract::Transcription factors have been used as neuronal markers in the nucleus accumbens (ACB) of male juvenile spontaneously hypertensive rats (SHR), an animal model of attention-deficit hyperactivity disorder (ADHD), to trace putative neural substrates. In SHR, immunocytochemistry and PC-assisted image analysis showed lowe...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-199705060-00011
更新日期:1997-05-06 00:00:00
abstract::Retinal light exposure induces several immediate-early genes in the hypothalamic suprachiasmatic nucleus (SCN), which contains the major circadian pacemaker of mammals. Clock-controlled and light-induced genes expressed in the SCN such as c- and contain upstream regulatory elements similar to those of the major immedi...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-200303030-00029
更新日期:2003-03-03 00:00:00
abstract::The localization of PKC gamma mRNA expression during the maturation of rat cerebellum has been studied by in situ hybridization. We found a transient expression over the granule cell layer and persistent high expression in the Purkinje cells during postnatal development. Expression in granule cells appeared as early a...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-199307000-00015
更新日期:1993-07-01 00:00:00
abstract::Interleukin-6 (IL-6) is a neurotrophic cytokine, however, its direct effect on nerve regeneration has not been well characterized. We therefore examined the effect of IL-6 on neurite regeneration using the rat dorsal root ganglion. IL-6 significantly enhanced neurite regeneration from transected nerve terminals. We al...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-200104170-00043
更新日期:2001-04-17 00:00:00
abstract::Hypoxia or global ischemia causes rapid loss of consciousness and a sudden increase in spontaneous transmitter release suggesting that coordinated synaptic activity is impaired. Gamma oscillations (30-100 Hz) are thought to provide for binding of parallel processed information in the brain, contributing to cognition a...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/WNR.0b013e3282f13e4f
更新日期:2007-11-19 00:00:00
abstract::Hyperactivity of the hypothalamic-pituitary-adrenal axis and impairment of the central corticotropin-releasing factor system are factors in the pathogenesis of depression. Though several antagonists of the corticotropin-releasing factor 1 receptor were effective in the recognized behavioral tests for antidepressant ac...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/WNR.0000000000001331
更新日期:2020-01-08 00:00:00
abstract::Opioid-receptor-like I (ORLI) receptors, ORLI mRNA and nociceptin are particularly abundant in the limbic system and in central monoaminergic areas, brain regions involved in mood regulation and response to antidepressants. To analyse whether ORLI receptors adaptations occur during the first 3 weeks of an antidepressa...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-200201210-00026
更新日期:2002-01-21 00:00:00
abstract::(-)-Epigallocatechin-3-gallate (EGCG) is the most abundant polyphenolic extract in green tea and it has attracted increasing attention for its multiple bioactive effects. However, the mechanisms by which EGCG exerts its neuroprotective actions in Alzheimer's disease (AD) are presently lacking. In the present study, a ...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/WNR.0000000000000803
更新日期:2017-07-05 00:00:00
abstract::We investigated the effect of nicotine, a nicotinic agonist, and D-cycloserine (DCS, a partial glycine-B agonist of the N-methyl-D-aspartate (NMDA) receptor complex) on aging-induced defects of water maze (WM) spatial navigation in rats. Nicotine (0.3 mg kg-1, s.c.) or DCS (10 mg kg-1, i.p.) enhanced acquisition of th...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-199702100-00024
更新日期:1997-02-10 00:00:00
abstract::The alpha 2-macroglobulin receptor/low-density lipoprotein receptor-related protein (alpha 2-M-R/LRP) is a multifunctional receptor which has been implicated in lipoprotein metabolism, clearance of proteinase-proteinase inhibitor complexes and regulation of growth factor/cytokine metabolism. This receptor is abundantl...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-199612200-00030
更新日期:1996-12-20 00:00:00
abstract::Abnormal sleep architecture and white matter hyperintensities (WMHs) may be involved in cognitive impairment. There is little evidence on the connections between WMHs and chronic insomnia disorder (CID). Therefore, we evaluated the severity of WMHs and polysomnography parameters as well as cognitive tests to explore t...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/WNR.0000000000001232
更新日期:2019-06-12 00:00:00
abstract::Musical expertise has been shown to induce widespread structural and functional alterations in the brain, even-handedly affecting top-down and bottom-up factors. At the same time, it is known that the early evoked gamma-band response (GBR) can be modulated by top-down as well as bottom-up factors such as attention and...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/WNR.0b013e328360abdb
更新日期:2013-06-19 00:00:00
abstract::An fMRI investigation was conducted to determine whether patients with impaired face recognition, a deficit known as prosopagnosia, would show functional activation in the fusiform gyrus, the neural substrate for face processing, when viewing faces. While the patients did show activation in the fusiform gyrus, with si...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-200106130-00014
更新日期:2001-06-13 00:00:00
abstract::To clarify possible interaction between the ventral hippocampal cannabinoid CB2 receptors and the cholinergic system in control of the memory process, the effects of cannabinoid and acetylcholine receptor agents on memory consolidation have been investigated in mice. Animals implanted with bilateral cannulas at the CA...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/WNR.0000000000001381
更新日期:2020-01-27 00:00:00
abstract::Neuronotrophic factors (e.g. basic fibroblast growth factor, bFGF and nerve growth factor, NGF) have been demonstrated to respectively promote survival and neuritogenesis in cultures of dissociated adult rat spiral ganglia. Transforming growth factor beta (TGF beta 1) has been shown to modulate the response of culture...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-199204000-00001
更新日期:1992-04-01 00:00:00
abstract::Leptin in the rat's nucleus accumbens (NAcc) core has previously shown to disrupt the effects of acute administration of cocaine on both locomotor activity and the phosphorylation levels of glycogen synthase kinase 3β. In the present study, we further measured the phosphorylation levels of GluA1 after bilateral microi...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/WNR.0000000000001001
更新日期:2018-04-11 00:00:00
abstract::Changes in the regulation, formation, and gating of connexin-based gap junction channels occur in various disorders. It has been shown that H and Ca are involved in the regulation of gap junctional communication. Ischemia-induced intracellular acidification and Ca overload lead to closure of gap junctions and inhibit ...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/WNR.0000000000000746
更新日期:2017-03-01 00:00:00
abstract::Psychotic-like episodes in divers exposed to high pressure have been attributed to either the high-pressure neurological syndrome, confinement in pressure chamber, the subject's personality, or the addition of nitrogen or hydrogen to the basic helium-oxygen breathing mixture used for deep diving. Alternatively, it is ...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-199503000-00027
更新日期:1995-03-07 00:00:00
abstract::In situ hybridization has demonstrated mRNA for olfactory receptors (OR) in the axon terminals of olfactory receptor neurons. Neurons that express the same OR appear to send their axons to two stereotyped glomeruli in the olfactory bulb (OB). Based on these observations, we tested the feasibility of using RT-PCR to is...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/00001756-199811160-00032
更新日期:1998-11-16 00:00:00
abstract::Müller cells, as the predominant glial element in the sensory retina, play a crucial role in healthy and diseased retina. Overactivation of Müller cells in response to damage is detrimental to the retina tissue. Current research shows that inhibiting glial fibrillary acidic protein (GFAP), a sensitive indicator of Mül...
journal_title:Neuroreport
pub_type: 杂志文章
doi:10.1097/WNR.0000000000001143
更新日期:2018-12-05 00:00:00