Abstract:
CONTEXT:The filtrate of Staphylococcus aureus culture, named staphylococcal enterotoxin C injection, has been used for 10 years in China. SEC2 has been claimed to be the only staphylococcal enterotoxins (SEs) without certifiable evidence. OBJECTIVES:To present an efficient procedure for the expression and purification of recombinant proteins SEG and SEI, from S. aureus. MATERIALS AND METHODS:In present work, we extracted total DNA from S. aureus (FRI 1230) and the recombinant proteins of SEG and SEI were then cloned, expressed and purified using E. coli. Splenic lymphocytes were used as effector cells and K562 and B16 cells were used as target cells to evaluate the inhibitory and stimulatory abilities of purified rSEG and rSEI on in vitro proliferation. RESULTS:The size of amplified products of SEG and SEI genes were found to be about 400 and 467 bp, respectively. pGEX-SEG and pGEX-SEI were constructed successfully. SEG and SEI were demonstrated to be active stimulators of T-cell proliferation; moreover, they inhibited the proliferation of K562 cells and B16 cells. DISCUSSION:The current findings suggest that SEC2 might not be the only active component of staphylococcal enterotoxin C injection and may involve other essential proteins like SEG and SEI in its clinical efficacy. CONCLUSION:This efficient procedure for the expression and purification of SEG and SEI and may be useful for mass production of therapeutically important proteins. In the future, proteins acting as active stimulators of T-cell proliferation may help in developing effective cancer therapy.
journal_name
Pharm Bioljournal_title
Pharmaceutical biologyauthors
Chen B,Akash MS,Rehman K,Sun H,Chen Sdoi
10.3109/13880209.2013.804845subject
Has Abstractpub_date
2014-01-01 00:00:00pages
8-13issue
1eissn
1388-0209issn
1744-5116journal_volume
52pub_type
杂志文章abstract::Context:Caragana pruinosa Kom. (Fabaceae), a commonly used folk medicine, has been found to possess antitumor effects. However, the antiproliferative effect of 2,4-dihydroxy-3'-methoxy-4'-ethoxychalcone (DMEC) derived from C. pruinosa against multiple myeloma (MM) has never been investigated. Objective: This study sys...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.1080/13880209.2019.1662814
更新日期:2019-12-01 00:00:00
abstract:CONTEXT:Glycyrrhiza glabra L. (Febaceae) has been widely used in traditional medicine and scientifically explored for its anticonvulsant and memory improving potential. OBJECTIVE:The objective of this study is to investigate the effect of flavonoid rich fraction of G. glabra root extract against phenytoin-induced cogn...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2015.1063673
更新日期:2016-07-01 00:00:00
abstract::Heliopsis longipes S.F. Blake (Asteraceae: Heliantheae) (chilcuague) is used in Mexican traditional medicine against parasites and to alleviate tooth and muscle pains. Its biocide effect has already been experimentally demonstrated; however, its analgesic action and its action on the nervous system (NS) have not been ...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880200903078495
更新日期:2010-02-01 00:00:00
abstract:CONTEXT:Cyclophosphamide (CP) causes lung injury in rats through its ability to generate free radicals with subsequent epithelial and endothelial cell damage. OBJECTIVE:This study was conducted to assess whether allicin can ameliorate CP-induced early lung injury in rats. MATERIALS AND METHODS:Male Sprague Dawely rat...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2013.766895
更新日期:2013-06-01 00:00:00
abstract:CONTEXT:Commiphora myrrha (Burseraceae), a shrub resembling a small tree, has been used for several centuries for the treatment of various diseases. OBJECTIVE:This study investigates the hepatoprotective activity of C. myrrha ethanol extract against d-galactosamine/lipopolysaccharide (d-GalN/LPS)-induced acute hepatic...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2015.1005754
更新日期:2015-01-01 00:00:00
abstract:CONTEXT:The content of withanolides in the roots of Withania somnifera (L.) Dunal (Solanaceae) is important for therapeutic application. Earlier studies have shown that the deficiency of macro- and micronutrients affects the growth of W. somnifera. Therefore, we examined the effect of these deficiencies on the withanol...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2014.931439
更新日期:2015-04-01 00:00:00
abstract:UNLABELLED:Abstract Context: Solute carrier transporters (SLCs) are membrane proteins responsible for cellular influx of various substances including many pharmaceutical agents; therefore, they largely impact on drug disposition and elimination in body. Punica granatum Linnaeus (Lythraceae), pomegranate, is a fruit wit...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2014.900809
更新日期:2014-12-01 00:00:00
abstract:CONTEXT:Diabetes mellitus is characterized by oxidative stress, which in turn induces endothelial dysfunction. As a potent antioxidant compound, lycopene might rescue diabetic endothelial dysfunction by reducing oxidative stress. OBJECTIVE:The present study investigated whether lycopene could lower oxidative stress an...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2011.574707
更新日期:2011-11-01 00:00:00
abstract:CONTEXT:Pomegranate [Punica granatum L. (Lythraceae)] is rich in antioxidants. OBJECTIVE:The present work was undertaken to evaluate the protective effects of methanol extract of pomegranate peel extract (PPE) against a rat model of oxidative stress generated by treatment with mercuric chloride (HgCl(2)). MATERIALS A...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2012.738333
更新日期:2013-04-01 00:00:00
abstract:CONTEXT:Amaranthus spinosus Linn. (Amaranthaceae), commonly known as ''spiny pigweed'', is used in both Indian traditional system and folk medicine for treatment of infectious diseases for a long time in several traditional herbal medicinal preparations. A novel fatty acid [(14E, 18E, 22E, 26E)-methyl nonacosa-14, 18, ...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2016.1155628
更新日期:2016-10-01 00:00:00
abstract:CONTEXT:Hyperlipidemia is known to be a major risk factor for the development of cardiovascular diseases (CVDs) which include atherosclerosis, coronary heart disease, and stroke. Although there are a large number of anti-hyperlipidemic drugs available, unfortunately, they all have side effects. OBJECTIVE:Terminalia ch...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2014.962058
更新日期:2015-08-01 00:00:00
abstract:CONTEXT:Recent studies have shown compound Danshen dripping pills (CDDP) could improve microcirculation in ischemic/reperfusion injury and other microvascular disorders. The mechanism for CDDP's role in microcirculation is not clear. OBJECTIVE:To explore the protective effects of CDDP on microvascular dysfunction. MA...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.1080/13880209.2020.1790619
更新日期:2020-12-01 00:00:00
abstract:CONTEXT:Carissa edulis Vahl (Apocynaceae) is used in Nigerian folk medicine to manage a plethora of diseases including epilepsy, cancer, and inflammation; its efficacy is widely acclaimed among communities of northern Nigeria. OBJECTIVE:This study establishes anticonvulsant activities of aqueous fraction of ethanol ro...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2014.981280
更新日期:2015-01-01 00:00:00
abstract::Context Acetaminophen (APAP), also known as paracetamol and N-acetyl p-aminophenol, is one of the most frequently used drugs for analgesic and antipyretic purposes on a worldwide basis. It is safe and effective at recommended doses but has the potential for causing hepatotoxicity and acute liver failure (ALF) with ove...
journal_title:Pharmaceutical biology
pub_type: 杂志文章,评审
doi:10.3109/13880209.2016.1150302
更新日期:2016-10-01 00:00:00
abstract:CONTEXT:The roots of Phytolacca americana L. (Phytolaccaceae) may be toxic. Despite heated controversy over the toxic compounds of P. americana, especially esculentosides, relevant studies remain scarce. OBJECTIVE:The objective of this study is to screen the toxic fractions and compounds of P. americana, to determine ...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2015.1016182
更新日期:2016-01-01 00:00:00
abstract::Context Phyllanthus emblica L. (Euphorbiaceae) (amla), Manilkara zapota L.P. Royen (Sapotaceae) (sapota) and silymarin are reported to contain antioxidant effects. However, information on other biological activities relating to the anti-aging properties is limited. Objective To compare in vitro antioxidants, anti-coll...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2015.1133658
更新日期:2016-09-01 00:00:00
abstract:CONTEXT:The resistance of bacteria to antibiotics is raising serious concern globally. Asian medicinal plants could improve the current treatment strategies for bacterial infections. The antibacterial properties of medicinal plants used by the Khyang tribe in Bangladesh have not been investigated. OBJECTIVE:The presen...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.1080/13880209.2018.1446030
更新日期:2018-12-01 00:00:00
abstract:CONTEXT:Light microscopy is in most cases a quick method for the identification and discrimination of medicinally used plant drugs; moreover, this technique is very inexpensive. Reliable descriptions of the anatomy of plants and their adulterations are prerequisites for necessary purity controls. OBJECTIVE:The anatomy...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2010.548390
更新日期:2011-08-01 00:00:00
abstract::Context: Curcumin, a polyphenolic compound extracted from the rhizome of the tropical plant Curcuma longa L. (Zingiberaceae), has been considered as a cancer chemopreventive drug by American National Cancer Institute.Objective: To examine the effect of curcumin on acute monocytic leukaemia SHI-1 cells in vivo.Material...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.1080/13880209.2019.1701042
更新日期:2020-12-01 00:00:00
abstract:CONTEXT:Ageratum conyzoides Linn. (Asteraceae) is an annual herbaceous plant with a long history of traditional medicinal and agricultural uses; it is usually grown in the northeast part of Bangladesh. OBJECTIVE:The ethanol extract of the plant leaves was evaluated for preliminary phytochemical screening with its anti...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2013.770535
更新日期:2013-07-01 00:00:00
abstract:CONTEXT:Salvicine is a pharmacologically active derivative from Chinese medicinal plant Salvia prionitis Hance (Labiatae). It has been reported that salvicine inactivates β1 integrin and inhibits integrin-mediated cell adhesion to fibronectin. Given the emerging correlation between integrins and angiogenesis, we propos...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2013.776612
更新日期:2013-08-01 00:00:00
abstract:CONTEXT:Portulacerebroside A (PCA) is a novel cerebroside compound isolated from Portulaca oleracea L. (Portulacaceae), an edible and medicinal plant distributed in the temperate and tropical zones worldwide. OBJECTIVE:This study investigates the effects of PCA in human liver cancer HCCLM3 cells on metastasis and inva...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2014.941505
更新日期:2015-05-01 00:00:00
abstract::The activity of an (8-hydroxymethylen)-trieicosanyl acetate compound obtained from chloroform extracts of Senna villosa (Mill.) H.S. Irwin & Barneby (Leguminosae) against Trypanosoma cruzi was evaluated in vivo. Oral doses of 2.1, 8.4, and 33.6 microg/g were tested for 28 days in BALB/c mice infected with T. cruzi. Re...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880200903241853
更新日期:2010-06-01 00:00:00
abstract::Essential oil compositions and antioxidant potentials of fourteen ethanol (75%) root extracts prepared from twelve taxa of the genus Paeonia (Paeoniaceae), including P. arietina Anders., P. daurica Andrews, P. xkayae N. Ozhatay, P. kesrouanensis Thiéb., P. mascula (L.) Miller subsp. arasicola G. Kaynak, ö. Yilmaz & R....
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880200903029332
更新日期:2010-01-01 00:00:00
abstract::Context Yellow tea containing the same catechins as other types of tea but in different proportions has been suggested to possess potent anticancer activities. Objective This study investigates the chemopreventive effect of yellow tea aqueous extract against N-nitrosodiethylamine (NDEA)-induced liver carcinogenesis in...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2015.1137600
更新日期:2016-09-01 00:00:00
abstract:CONTEXT:Felicia muricata Thunb. (Nees) (Asteraceae) leaves are used in folklore medicine of South Africa as an oral remedy for pain and inflammation. However, the efficacy of the plant part is yet to be validated with scientific experiments. OBJECTIVE:The current study is an effort to investigate the anti-inflammatory...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880200903373664
更新日期:2010-09-01 00:00:00
abstract:CONTEXT:Virgin coconut oil (VCO) has been reported to possess antioxidative, anti-inflammatory and anti-stress properties. OBJECTIVE:Capitalizing on these therapeutic effects, this study investigated for the first time the potential of VCO on memory improvement in vivo. MATERIALS AND METHODS:Thirty male Wistar rats (...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.1080/13880209.2017.1280688
更新日期:2017-12-01 00:00:00
abstract:CONTEXT:Millettia barteri (Benth.) Dunn (Fabaceae) is an African medicinal plant used in folk medicine to treat many diseases. This species, as well as other Mellettia species, has been of interest to researchers because of their wide range of traditional uses. OBJECTIVE:Phytochemical, antimicrobial and 2,2-diphenyl-1...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2011.579618
更新日期:2012-02-01 00:00:00
abstract::Context: Puerarin and triptolide are sometimes used together for the treatment of disease in Chinese clinics; however, the drug-drug interaction between puerarin and triptolide is still unknown. Objective: This study investigates the effects of puerarin on the pharmacokinetics of triptolide in rats and clarifies its m...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.1080/13880209.2019.1626448
更新日期:2019-12-01 00:00:00
abstract:CONTEXT:Diabetic nephropathy is one of the important microvascular complications of diabetes; however, the main problem remains is the control of progression of nephropathy in diabetes. Chebulic acid was selected, as tannins from Terminalia chebula are used as antidiabetic, renoprotective, antioxidant, hypotensive and ...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2012.698288
更新日期:2013-01-01 00:00:00