Abstract:
:Context:Caragana pruinosa Kom. (Fabaceae), a commonly used folk medicine, has been found to possess antitumor effects. However, the antiproliferative effect of 2,4-dihydroxy-3'-methoxy-4'-ethoxychalcone (DMEC) derived from C. pruinosa against multiple myeloma (MM) has never been investigated. Objective: This study systematically evaluates the antiproliferative effect of DMEC against MM cells. Materials and methods: The antiproliferative effect of DMEC (1, 2, 4, 8, 16, 32, and 64 μM) on MM cells lines, including RPMI8226, MM.1S, and U266, was examined using Cell counting kit-8 (CCK-8) assay after 24 h incubation. The proapoptotic effect of DMEC (20 μM) was determined using fluorescent microscope and flow cytometer, and its possible underlying mechanisms were further studied by using western blotting analysis. Results: The half maximal inhibitory concentrations (IC50) of DMEC on RPMI8226, MM.1S, and U266 cells were calculated as 25.97, 18.36, and 15.02 μM, respectively. The inhibitory effect of DMEC on MM cells was related to mitochondria-mediated apoptosis via upregulation of the cleaved-caspase-3 (C-3), cleaved-caspase-9 (C-9), Bad, and cytochrome C (Cyto C), but downregulation of the Bcl-2 and poly ADP-ribose polymerase (PARP). Furthermore, DMEC (5, 10, and 20 μM) reduced the expression of phosphatidylinositol-3-kinase (PI3K), phosphorylated (p)-protein kinase B (Akt), and p-mammalian target of rapamycin (p-mTOR), which were further evidenced by pretreatment with IGF-1, a PI3K activator. Conclusion: Collectively, our results indicate that the DMEC could be treated as a new candidate for treatment of multiple myeloma in the future. Also, an in vivo study is warranted in the future.
journal_name
Pharm Bioljournal_title
Pharmaceutical biologyauthors
Zhu F,Jiang D,Zhang M,Zhao Bdoi
10.1080/13880209.2019.1662814subject
Has Abstractpub_date
2019-12-01 00:00:00pages
641-648issue
1eissn
1388-0209issn
1744-5116journal_volume
57pub_type
杂志文章abstract:CONTEXT:Different parts of Punica granatum Linn. (Punicaceae) are traditionally used as renal protective agents in the Indian system of medicine. However, there is paucity of information regarding its role in diabetic nephropathy. OBJECTIVE:The present study investigates the nephroprotective potential of flavonoid-ric...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2014.910533
更新日期:2015-01-01 00:00:00
abstract:CONTEXT:The pharmaceutical alkaloid huperzine A (HupA), currently used in herbal supplements and medicines worldwide, is predominantly sourced from the Chinese lycopod Huperzia serrata (Thunb. ex Murray) Trev. (Lycopodiaceae), which on average contains only 0.08 mg HupA g(-1) dry weight, and is experiencing a rapid dec...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2010.485619
更新日期:2010-09-01 00:00:00
abstract:CONTEXT:Glycyrrhiza glabra L. (Febaceae) has been widely used in traditional medicine and scientifically explored for its anticonvulsant and memory improving potential. OBJECTIVE:The objective of this study is to investigate the effect of flavonoid rich fraction of G. glabra root extract against phenytoin-induced cogn...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2015.1063673
更新日期:2016-07-01 00:00:00
abstract:CONTEXT:Bombacaceae is a small family of the order Malvales and contains about 28 genera and 200 species. Members of this family are not only showy ornamentals but they possess significant economical and commercial reputation as well. In addition, various plant parts of several species are widely used as foods and trad...
journal_title:Pharmaceutical biology
pub_type: 杂志文章,评审
doi:10.3109/13880209.2012.698286
更新日期:2013-01-01 00:00:00
abstract:CONTEXT:Neuroprotective therapy to rescue dopaminergic neurons is an important trait in the management of Parkinson's disease (PD). OBJECTIVE:The present study identified and evaluated SFSE-T, a standardized hydroalcoholic extract of Trigonella foenum-graecum L. seeds (Fabaceae), in animal models of PD. MATERIALS AND...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2012.747547
更新日期:2013-05-01 00:00:00
abstract:CONTEXT:Vernonia amygdalina Del. (VA; Asteraceae or Compositae) is a small tree growing throughout tropical Africa. It is widely used for food and medicinal purposes by local people. It was reported that it had several qualities, including anticancer activity. OBJECTIVE:A sesquiterpene lactone, vernodalinol, was isola...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2010.523429
更新日期:2011-05-01 00:00:00
abstract::Rhinacanthus nasutus (L.) Kurz (Acanthaceae) has long been used in Thai traditional medicine for treatment of tinea versicolor, ringworm, pruritic rash, and abscess. The active constituents are known as a group of naphthoquinone esters, rhinacanthins. This work focused on establishment of R. nasutus root cultures and ...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880200903578747
更新日期:2010-11-01 00:00:00
abstract:CONTEXT:Virgin coconut oil (VCO) has been reported to possess antioxidative, anti-inflammatory and anti-stress properties. OBJECTIVE:Capitalizing on these therapeutic effects, this study investigated for the first time the potential of VCO on memory improvement in vivo. MATERIALS AND METHODS:Thirty male Wistar rats (...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.1080/13880209.2017.1280688
更新日期:2017-12-01 00:00:00
abstract:CONTEXT:Liposomes are increasingly employed to deliver chemotherapeutic agents, antisense oligonucleotides, and genes to various therapeutic targets. OBJECTIVE:The present investigation evaluates the ability of fusogenic pH-sensitive liposomes of rapamycin in increasing its antiproliferative effect on human breast ade...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2013.871640
更新日期:2014-07-01 00:00:00
abstract:CONTEXT:Silymarin (SM) is extracted from milk thistle Silybum marianum L. [Asteraceae (Compositae)] and known for antioxidative and anti-inflammatory effects. OBJECTIVE:The potential antidepressant-like effect of acute SM and possible involvement of nitric oxide (NO) were determined in male mice. MATERIAL AND METHODS...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2014.942787
更新日期:2015-05-01 00:00:00
abstract:CONTEXT:Growing adipose tissue is thought to require adipogenesis, angiogenesis, and extracellular matrix (ECM) remodeling. Close examination of developing adipose tissue microvasculature reveals that angiogenesis often precedes adipogenesis. Since our previous study demonstrated that Ob-X, the anti-angiogenic herbal c...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2010.547208
更新日期:2011-08-01 00:00:00
abstract:CONTEXT:Crataegus species are widely used as herbal medicines for preventing cardiovascular diseases (CVDs). However, there are no studies on the effects of Crataegus monogyna Jacq. (Rosaceae) and C. davisii Browicz on thrombosis, which is an important mechanism in CVDs. OBJECTIVE:The purpose of this study was to inve...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2014.914957
更新日期:2015-02-01 00:00:00
abstract::Abstract Context: Diabetes mellitus (DM) type 2 is a chronic disease characterized by hyperglycemia and insulin resistance. Oxidative stress participates in development and progression of DM, in which changes of superoxide dismutase (SOD) and catalase (CAT) were noted in DM mice. Berberine has been widely used as an a...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2013.839714
更新日期:2013-11-05 00:00:00
abstract:CONTEXT:Salsola imbricata Forssk. (Chenopodiaceae) has folkloric repute for the treatment of various gastrointestinal and respiratory ailments. OBJECTIVE:The present study investigates spasmolytic and bronchorelaxant effects of S. imbricata. MATERIALS AND METHODS:The crude aqueous-ethanol extract of the aerial parts ...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.1080/13880209.2017.1291691
更新日期:2017-12-01 00:00:00
abstract:CONTEXT:Cancer chemopreventive action of walnut [Juglans regia L. (Juglandaceae)] has been explored. OBJECTIVE:This study evaluated antiproliferative and antioxidant activities of walnut. MATERIALS AND METHODS:Various fractions of walnut extract have been screened for antiproliferative activity against human cancer c...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2010.537666
更新日期:2011-06-01 00:00:00
abstract:CONTEXT:The fruits of Aronia melanocarpa Elliot (Rosaceae), (black chokeberry), and Sambucus nigra L. (Caprifoliaceae), elderberries are rich in anthocyanins. Many studies have reported that anthocyanins are beneficial in diabetes due to their capacity to stimulate insulin secretion and reduce oxidative stress. OBJECT...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2014.931441
更新日期:2015-04-01 00:00:00
abstract:CONTEXT:The incidence and mortality of thrombotic disorders are rapidly increasing throughout the world. Therefore, attempts have been made to develop new anticoagulant and antithrombotic drugs. Our previous studies showed that a novel protein, named Fu-P, had fibrinogenolytic activity and much higher fibrinolytic acti...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2011.641023
更新日期:2012-07-01 00:00:00
abstract:CONTEXT:Polygonum species have been used in the treatment of several types of inflammatory disorders and cancer. Nevertheless, there are no reports related to the anti-inflammatory and anti-proliferative activities of Polygonum bellardii All. (Polygonaceae). OBJECTIVE:This study investigated the chemical composition o...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2013.775160
更新日期:2013-08-01 00:00:00
abstract:CONTEXT:Hippocratea africana (Willd.) Loes. ex Engl. (Celastraceae) root is used traditionally as an antipoison or antidote to treat liver diseases. OBJECTIVE:To evaluate antioxidative burst and hepatoprotective potentials of H. africana against paracetamol-induced liver injury in rats. MATERIALS AND METHOD:Antioxida...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2013.768273
更新日期:2013-07-01 00:00:00
abstract:CONTEXT:Silymarin, a flavonolignan from Silybum marianum (L.) Gaertn. (Asteraceae), has been reported to have antioxidant and anti-inflammatory properties. Therefore, it may be worthwhile to study the effect of silymarin on wound healing. OBJECTIVE:To evaluate the effect of silymarin on human fibroblast cells in an in...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2012.721789
更新日期:2013-03-01 00:00:00
abstract:CONTEXT:Stachys guyoniana Noë ex. Batt. and Mentha aquatica L. are two Algerian Lamiaceae used in folk medicine. OBJECTIVE:To investigate their antioxidant, anticholinesterase and antibacterial activities. MATERIAL AND METHODS:n-Butanol (BESG), ethyl acetate (EESG) and chloroform (CESG) extracts of S. guyoniana and m...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.1080/13880209.2016.1238488
更新日期:2017-12-01 00:00:00
abstract:CONTEXT:Diabetes mellitus is characterized by oxidative stress, which in turn induces endothelial dysfunction. As a potent antioxidant compound, lycopene might rescue diabetic endothelial dysfunction by reducing oxidative stress. OBJECTIVE:The present study investigated whether lycopene could lower oxidative stress an...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2011.574707
更新日期:2011-11-01 00:00:00
abstract:CONTEXT:Stress-induced changes in the dopaminergic system and subsequent enhancement of oxidative load and behavior are associated with a wide range of central and peripheral nervous disorders. Dopamine acts as a key neurotransmitter in the brain plays an important role in the regulation of motor and limbic functions. ...
journal_title:Pharmaceutical biology
pub_type: 杂志文章,评审
doi:10.3109/13880209.2013.837492
更新日期:2014-03-01 00:00:00
abstract:CONTEXT:Hypertrophic scarring, a common proliferative disorder of dermal fibroblasts, results from an overproduction of collagen and excessive deposition of extracellular matrix. Although the treatment with surgical excisions or steroid hormones can modify the symptoms, numerous treatment-related complications have als...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2010.542761
更新日期:2011-07-01 00:00:00
abstract:CONTEXT:The possibility of combining unripe plantain [Musa paradisiacae Linn (Plantaginaceae)] and cocoyam [Colocassia esculenta Linn (Araceae)] in the management of diabetes has not been investigated. OBJECTIVE:The objective of this study is to evaluate the antihyperglycemic and antihyperlipidemic actions of unripe p...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2015.1016181
更新日期:2016-01-01 00:00:00
abstract:CONTEXT:Dyslipidemia is one of the major risk factors for cardiovascular disease in diabetes mellitus (DM). The availability of multiple lipid-lowering drugs and supplements provides new opportunities for patients to regulate lipid levels. OBJECTIVE:The present study was designed to evaluate the effect of Butea monosp...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2013.876055
更新日期:2014-08-01 00:00:00
abstract::2-Formyl-1-hydroxyanthraquinone, along with ten other known anthraquinones (1-hydroxy-2-methylanthraquinone, nordamnacanthal, damnacanthal, lucidin-?-methyl ether, rubiadin, rubiadin-1-methyl ether, soranjidiol, morindone, morindone-5-methyl ether and alizarin-1-methyl ether), isolated from the roots of Morinda ellipt...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.1076/1388-0209(200009)3841-AFT298
更新日期:2000-01-01 00:00:00
abstract:CONTEXT:Fructus Meliae toosendan extracts (FMTE) have a good therapeutic effect on coccidiosis, but there is no relevant research on its prophylactic effect on coccidiosis. OBJECTIVE:This study comprehensively evaluates the anticoccidial effect of FMTE. MATERIALS AND METHODS:In vitro, the unsporulated oocysts were tr...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.1080/13880209.2020.1784234
更新日期:2020-12-01 00:00:00
abstract:CONTEXT:The fruit of the Prunus mume Sieb. et Zucc (Rosaceae) is used as a health food or medicinal material in traditional herb medicine for a long time in Eastern Asian countries. OBJECTIVE:Our present study investigated the hypouricemic effect of the methanol extract from P. mume fruit (MPMF) in mice with potassium...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.3109/13880209.2012.683115
更新日期:2012-11-01 00:00:00
abstract:CONTEXT:Clerodendrum viscosum Vent. (Verbenaceae) is a shrub, widely used amongst the natives of India against various diseases. OBJECTIVE:Crude extract of the plant was tested in vitro on a tapeworm Raillietina tetragona Molin (Davaineidae) to evaluate its potential anthelmintic efficacy and ultrastructural changes i...
journal_title:Pharmaceutical biology
pub_type: 杂志文章
doi:10.1080/13880209.2016.1226367
更新日期:2017-12-01 00:00:00