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Polyphenols from aerial parts of Polygonum bellardii and their biological activities.

Abstract：

CONTEXT:Polygonum species have been used in the treatment of several types of inflammatory disorders and cancer. Nevertheless, there are no reports related to the anti-inflammatory and anti-proliferative activities of Polygonum bellardii All. (Polygonaceae). OBJECTIVE:This study investigated the chemical composition of the methanol extract of P. bellardii. The anti-inflammatory and cytotoxic activities of methanol, n-butanol, ethyl acetate extracts and isolated polyphenols were determined. MATERIALS AND METHODS:The chemical structure of the isolated compounds was elucidated using different spectral techniques. MTT assay was used to evaluate the anti-proliferative activity in HeLa, MCF-7 and HepG-2 cells. Inhibition of 5-lipoxygenase (5-LOX) activity and prostaglandin E2 (PGE2) production in stimulated HepG-2 cells were used to assess the anti-inflammatory activity. RESULTS:The present study resulted in isolation of five compounds (new for the species). They were identified as gallic acid (1), quercetin (2), myricetin (3), quercetin-3-O-β-D-glucopyranoside (5) and myricetin-3-O-α-arabinofuranoside (7). Additionally, a couple of previously isolated compounds such as quercetin-3-O-(5″-acetyl-α-arabinofuranoside) (4) and myricetin-3-O-(5″-acetyl-α-arabinofuranoside) (6) were detected. The n-butanol extract has the highest cytotoxicity in HeLa, MCF-7 and HepG-2 cells, with IC₅₀ values of 15.26, 50.66 and 30.09 µg/ml, respectively. Compound 6 exhibited a marked cytotoxicity in HeLa (IC₅₀ 75.04 µg/ml) and HepG-2 (IC₅₀ 41.03 µg/ml) cells. Crude extracts and pure compounds inhibited the 5-LOX activity and PGE2 production in a dose-dependent manner (0.1-250 µg/ml). DISCUSSION AND CONCLUSION:These results explain the traditional uses of P. bellardii and indicate that polyphenols, despite structural similarity, have different cytotoxic and anti-inflammatory effects.

journal_name

Pharm Biol

journal_title

Pharmaceutical biology

authors

Abd El-Kader AM,El-Readi MZ,Ahmed AS,Nafady AM,Wink M,Ibraheim ZZ

doi

10.3109/13880209.2013.775160

subject

Has Abstract

pub_date

2013-08-01 00:00:00

pages

1026-34

issue

eissn

1388-0209

issn

1744-5116

journal_volume

pub_type

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Polyphenols from aerial parts of Polygonum bellardii and their biological activities.