Reversal effects of Rabdosia rubescens extract on multidrug resistance of MCF-7/Adr cells in vitro.

Abstract:

CONTEXT:Rabdosia rubescens (Hemsl.) Hara (Lamiaceae) is widely used in traditional Chinese medicines for the treatment of antitumor, antimicrobial, anti-inflammatory and antioxidation. It is also used as a supplement in the treatment of many cancers, such as esophagus, mammary gland, liver and prostate cancers. OBJECTIVE:To investigate the multidrug resistance (MDR) reversal effects and its possible mechanism of R. rubescens extracts on human breast cancer cell line MCF-7/Adr (Michigan Cancer Foundation--7/adriamycin resistance). MATERIALS AND METHODS:Rabdosia rubescens were extracted by reflux extraction method with different solvent such as petroleum ether, chloroform, ethyl acetate, n-butyl alcohol and water in order and obtain petroleum ether fraction (PEF), chloroform fraction (CF), ethyl acetate fraction (EAF), n-butyl alcohol fraction (BAF) and aqueous fraction (AF). The active extract fractions of R. rubescens were screened by rhodamine123 (Rh123) accumulation assay. Cytotoxicity of the effect fraction was examined by the MTT assay; the intracellular accumulation of adriamycin and expression of P-gp were examined by flow cytometry; the gene transcription of MDR1 was determined by RT-PCR. RESULTS:CF and EAF fractions could increase the intracellular accumulation of adriamycin in MCF-7/Adr cells, PEF, BAF and AF fractions showed little effect on the intracellular accumulation of adriamycin or Rh123. When adriamycin was used in combination with CF and EAF fractions at non-toxic concentration on MCF-7/Adr cells, CF and EAF fractions can reverse MDR of MCF-7/Adr cells, and the reverse folds were 2.16 (CF, 4 μg/mL), 4.60 (CF, 20 μg/mL), 1.87 (EAF, 4 μg/mL) and 4.02 (EAF, 20 μg/mL), respectively. After treatment with CF (4.20 μg/mL) and EAF (4.20 μg/mL) for 48 h, the MDR1 gene expression level in MCF-7/Adr cells was decreased by 40.17, 48.14, 33.86 and 42.52%, and the abundance of P-gp also decreased by 8.63, 24.53, 27.50 and 34.91% in MCF-7/Adr cells, respectively. DISCUSSION AND CONCLUSION:These results indicate the therapeutic value of chloroform fraction (CF) and ethyl acetate fraction (EAF) from R. rubescens as potential MDR reversing agents and warranted further investigation.

journal_name

Pharm Biol

journal_title

Pharmaceutical biology

authors

Li F,Fan J,Wu Z,Liu RY,Guo L,Dong Z,Wang Z

doi

10.3109/13880209.2013.784342

subject

Has Abstract

pub_date

2013-09-01 00:00:00

pages

1196-203

issue

9

eissn

1388-0209

issn

1744-5116

journal_volume

51

pub_type

杂志文章
  • Protective effect on phenytoin-induced cognition deficit in pentylenetetrazol kindled mice: A repertoire of Glycyrrhiza glabra flavonoid antioxidants.

    abstract:CONTEXT:Glycyrrhiza glabra L. (Febaceae) has been widely used in traditional medicine and scientifically explored for its anticonvulsant and memory improving potential. OBJECTIVE:The objective of this study is to investigate the effect of flavonoid rich fraction of G. glabra root extract against phenytoin-induced cogn...

    journal_title:Pharmaceutical biology

    pub_type: 杂志文章

    doi:10.3109/13880209.2015.1063673

    authors: Singh P,Singh D,Goel RK

    更新日期:2016-07-01 00:00:00

  • Antimicrobial bioassay-guided fractionation of a methanol extract of Eupatorium triplinerve.

    abstract:CONTEXT:Eupatorium triplinerve Vahl (Asteraceae), popularly known as Japana, is widely used in folk medicine, due its analgesic, anticoagulant, antianorexic, antiparasitic, anthelmintic, sedative, antifungal, and antibacterial properties. OBJECTIVE:The present study evaluated the chemical composition and antimicrobial...

    journal_title:Pharmaceutical biology

    pub_type: 杂志文章

    doi:10.3109/13880209.2014.948634

    authors: Matos Lopes TR,de Oliveira FR,Malheiros FF,de Andrade MA,Monteiro MC,Baetas Gonçalves AC

    更新日期:2015-06-01 00:00:00

  • Bioassay-guided isolation of antioxidant agents with analgesic properties from flowers of Tagetes patula.

    abstract:CONTEXT:Tagetes patula L. is one of the French marigold group of the Asteraceae family. It is recognized in folklore for its medicinal and pesticidal properties. OBJECTIVE:In search of more effective, but non-toxic compounds with antioxidative potential led to the bioassay guided isolation studies on the extracts of T...

    journal_title:Pharmaceutical biology

    pub_type: 杂志文章

    doi:10.3109/13880209.2010.523006

    authors: Faizi S,Dar A,Siddiqi H,Naqvi S,Naz A,Bano S,Lubna

    更新日期:2011-05-01 00:00:00

  • Effects of puerarin on the pharmacokinetics of triptolide in rats.

    abstract::Context: Puerarin and triptolide are sometimes used together for the treatment of disease in Chinese clinics; however, the drug-drug interaction between puerarin and triptolide is still unknown. Objective: This study investigates the effects of puerarin on the pharmacokinetics of triptolide in rats and clarifies its m...

    journal_title:Pharmaceutical biology

    pub_type: 杂志文章

    doi:10.1080/13880209.2019.1626448

    authors: Wang Q,Wu Y,Xiang F,Feng Y,Li Z,Ding Y

    更新日期:2019-12-01 00:00:00

  • Changes in the contents of main secondary metabolites in two Turkish Hypericum species during plant development.

    abstract:CONTEXT:The genus Hypericum (Guttiferae) has received considerable scientific interest as a source of biologically active compounds. OBJECTIVE:The study determined the morphogenetic and ontogenetic variation in the main bioactive compounds of two Hypericum species, namely, Hypericum aviculariifolium subsp. depilatum v...

    journal_title:Pharmaceutical biology

    pub_type: 杂志文章

    doi:10.3109/13880209.2012.733012

    authors: Cirak C,Radusiene J,Camas N,Caliskan O,Odabas MS

    更新日期:2013-03-01 00:00:00

  • Plants used to treat skin diseases in northern Maputaland, South Africa: antimicrobial activity and in vitro permeability studies.

    abstract:CONTEXT:Ethnobotanical claims of medicinal plants used in northern Maputaland are limited. Objectives To establish scientific validity for a selection of the plants used in Maputaland to treat skin diseases. MATERIALS AND METHODS:Aqueous and dichloromethane-methanol extracts were prepared from 37 plant species which w...

    journal_title:Pharmaceutical biology

    pub_type: 杂志文章

    doi:10.3109/13880209.2016.1158287

    authors: Nciki S,Vuuren S,van Eyk A,de Wet H

    更新日期:2016-11-01 00:00:00

  • Therapeutic potential of gossypol: an overview.

    abstract:CONTEXT:Polyphenols are naturally occurring compounds found in fruits, vegetables, cereals, and beverages. Polyphenols occupy a unique place in biological science for their pharmacological properties. Gossypol is a polyphenolic compound that has attracted attention because of its biological effects. OBJECTIVE:Gossypol...

    journal_title:Pharmaceutical biology

    pub_type: 杂志文章,评审

    doi:10.3109/13880209.2013.832776

    authors: Keshmiri-Neghab H,Goliaei B

    更新日期:2014-01-01 00:00:00

  • Development and validation of HPLC method for vicenin-1 isolated from fenugreek seeds in rat plasma: application to pharmacokinetic, tissue distribution and excretion studies.

    abstract:CONTEXT:Vicenin-1, a flavonol glycoside, has potent platelet aggregation inhibition, antioxidant, radioprotectants and anti-inflammatory activities. OBJECTIVE:To establish a rapid, simple, precise and sensitive high-performance liquid chromatography (HPLC) method for determination of vicenin-1 in rat plasma, and to in...

    journal_title:Pharmaceutical biology

    pub_type: 杂志文章

    doi:10.3109/13880209.2016.1172245

    authors: Kandhare AD,Bodhankar SL,Mohan V,Thakurdesai PA

    更新日期:2016-11-01 00:00:00

  • Stress-mediated modulations in dopaminergic system and their subsequent impact on behavioral and oxidative alterations: an update.

    abstract:CONTEXT:Stress-induced changes in the dopaminergic system and subsequent enhancement of oxidative load and behavior are associated with a wide range of central and peripheral nervous disorders. Dopamine acts as a key neurotransmitter in the brain plays an important role in the regulation of motor and limbic functions. ...

    journal_title:Pharmaceutical biology

    pub_type: 杂志文章,评审

    doi:10.3109/13880209.2013.837492

    authors: Alghasham A,Rasheed N

    更新日期:2014-03-01 00:00:00

  • Antinociceptive and antioxidant potential of the crude ethanol extract of the leaves of Ageratum conyzoides grown in Bangladesh.

    abstract:CONTEXT:Ageratum conyzoides Linn. (Asteraceae) is an annual herbaceous plant with a long history of traditional medicinal and agricultural uses; it is usually grown in the northeast part of Bangladesh. OBJECTIVE:The ethanol extract of the plant leaves was evaluated for preliminary phytochemical screening with its anti...

    journal_title:Pharmaceutical biology

    pub_type: 杂志文章

    doi:10.3109/13880209.2013.770535

    authors: Hossain H,Karmakar UK,Biswas SK,Shahid-Ud-Daula AF,Jahan IA,Adnan T,Chowdhury A

    更新日期:2013-07-01 00:00:00

  • Molluscicidal activity of Solanum elaeagnifolium seeds against Galba truncatula intermediate host of Fasciola hepatica: Identification of β-solamarine.

    abstract:CONTEXT:The persistence of fascioliasis in many developing countries urges the search for simple, cheap, and effective substances. In this view, plants provide interesting molluscicidal activities thanks to the secondary metabolites they produce. The genus Solanum is known for its potent effect on vector snails. OBJEC...

    journal_title:Pharmaceutical biology

    pub_type: 杂志文章

    doi:10.3109/13880209.2015.1073332

    authors: Njeh F,Feki H,Koubaa I,Hamed N,Damak M,Ayadi A,Hammami H,Mezghani-Jarraya R

    更新日期:2016-01-01 00:00:00

  • Antioxidant and drug detoxification potential of aqueous extract of Annona senegalensis leaves in carbon tetrachloride-induced hepatocellular damage.

    abstract:CONTEXT: Despite the myriad uses of Annona senegalensis Pers. (Annonaceae) leaves in folklore medicine of Nigeria, the basis is yet to be substantiated by scientific investigations. OBJECTIVES: To investigate the antioxidant (in vitro and in vivo) and drug detoxification potential of aqueous extract of A. senegalensis...

    journal_title:Pharmaceutical biology

    pub_type: 杂志文章

    doi:10.3109/13880209.2010.483247

    authors: Ajboye TO,Yakubu MT,Salau AK,Oladiji AT,Akanji MA,Okogun JI

    更新日期:2010-12-01 00:00:00

  • Antinociceptive and anxiolytic-like effects of a neo-clerodane diterpene from Salvia semiatrata aerial parts.

    abstract:CONTEXT:Salvia semiatrata Zucc. (Lamiaceae) is a species used as a tranquilizer and to relieve pain in folk medicine in Santiago Huauclilla, Oaxaca, Mexico. OBJECTIVE:To evaluate the antinociceptive and anxiolytic-like effects of S. semiatrata extracts and identify a bioactive metabolite. MATERIALS AND METHODS:The ex...

    journal_title:Pharmaceutical biology

    pub_type: 杂志文章

    doi:10.1080/13880209.2020.1784235

    authors: Ortiz-Mendoza N,Zavala-Ocampo LM,Martínez-Gordillo MJ,González-Trujano ME,Peña FAB,Rodríguez IJB,Chávez JAR,Dorazco-González A,Aguirre-Hernández E

    更新日期:2020-12-01 00:00:00

  • Chemical composition and antibacterial activity of the essential oils from flower, leaf and stem of Ferula cupularis growing wild in Iran.

    abstract:CONTEXT:Ferula cupularis (Boiss.) Spalik et S. R. Downie (Apiaceae) is a common plant in Iran that grows in the foothills of Dena Mountain. In traditional folk medicine, this plant has different applications, but there are no studies proving their uses. OBJECTIVE:This study is the first attempt to investigate the chem...

    journal_title:Pharmaceutical biology

    pub_type: 杂志文章

    doi:10.3109/13880209.2014.924149

    authors: Alipour Z,Taheri P,Samadi N

    更新日期:2015-04-01 00:00:00

  • Comparative anatomical study on argentine species of lippia known as "poleo".

    abstract::Nine species belonging to the genus Lippia - Verbenaceae - are used in Argentine folk medicine. Four of them are known under the trivial name "poleo": L. turbinata Griseb., L. fissicalyx Troncoso, L. integrifolia (Griseb.) Hieron. and L. grisebachiana Mold. Mainly, their leaves are employed as digestive, stomaquique, ...

    journal_title:Pharmaceutical biology

    pub_type: 杂志文章

    doi:10.1076/1388-0209(200004)3821-1FT120

    authors: Bassols GB,Gurni AA

    更新日期:2000-01-01 00:00:00

  • Chrysanthemum boreale flower floral water inhibits platelet-derived growth factor-stimulated migration and proliferation in vascular smooth muscle cells.

    abstract:CONTEXT:Chrysanthemum boreale Makino (Compositae) (CBM) is a traditional medicine that has been used for the prevention or treatment of various disorders; it has various properties including antioxidation, anti-inflammation, and antitumor. OBJECTIVE:The present study was designed to explore the in vitro effect of CBM ...

    journal_title:Pharmaceutical biology

    pub_type: 杂志文章

    doi:10.3109/13880209.2014.941882

    authors: Kim DY,Won KJ,Yoon MS,Yu HJ,Park JH,Kim B,Lee HM

    更新日期:2015-05-01 00:00:00

  • Raspberry ketone, a naturally occurring phenolic compound, inhibits adipogenic and lipogenic gene expression in 3T3-L1 adipocytes.

    abstract:CONTEXT:Raspberry ketone (RK) is a natural phenolic compound of red raspberry. The dietary intake of RK has been reported to exert anti-obese actions and alter the lipid metabolism in vivo and human studies. OBJECTIVE:To elucidate a possible mechanism for anti-obese actions of RK, the effects of RK on the adipogenic a...

    journal_title:Pharmaceutical biology

    pub_type: 杂志文章

    doi:10.3109/13880209.2014.946059

    authors: Park KS

    更新日期:2015-06-01 00:00:00

  • Anthelmintic efficacy of Clerodendrum viscosum on fowl tapeworm Raillietina tetragona.

    abstract:CONTEXT:Clerodendrum viscosum Vent. (Verbenaceae) is a shrub, widely used amongst the natives of India against various diseases. OBJECTIVE:Crude extract of the plant was tested in vitro on a tapeworm Raillietina tetragona Molin (Davaineidae) to evaluate its potential anthelmintic efficacy and ultrastructural changes i...

    journal_title:Pharmaceutical biology

    pub_type: 杂志文章

    doi:10.1080/13880209.2016.1226367

    authors: Nandi S,Ukil B,Roy S,Kundu S,Lyndem LM

    更新日期:2017-12-01 00:00:00

  • Sacchachitin, a novel chitin-polysaccharide conjugate macromolecule present in Ganoderma lucidum: purification, composition, and properties.

    abstract:CONTEXT:The extraction method and the crude wound healing effects of sacchachitin from Ganoderma tsugae Murr. (Ganodermataceae) has been cited. However, its purity is still largely limited. OBJECTIVE:An improvement of the fractionation protocol to purify the sacchachitin from Ganoderma lucidum L. (Ganodermataceae) (SG...

    journal_title:Pharmaceutical biology

    pub_type: 杂志文章

    doi:10.3109/13880209.2012.711840

    authors: Chuang CM,Wang HE,Chang CH,Peng CC,Ker YB,Lai JE,Chen KC,Peng RY

    更新日期:2013-01-01 00:00:00

  • Antithrombotic effect of a novel protein from Fusarium sp. CPCC 480097 in a rat model of artery-vein bypass thrombosis.

    abstract:CONTEXT:The incidence and mortality of thrombotic disorders are rapidly increasing throughout the world. Therefore, attempts have been made to develop new anticoagulant and antithrombotic drugs. Our previous studies showed that a novel protein, named Fu-P, had fibrinogenolytic activity and much higher fibrinolytic acti...

    journal_title:Pharmaceutical biology

    pub_type: 杂志文章

    doi:10.3109/13880209.2011.641023

    authors: Wu B,Xu J

    更新日期:2012-07-01 00:00:00

  • In vitro synergism of a water insoluble fraction of Uncaria tomentosa combined with fluconazole and terbinafine against resistant non-Candida albicans isolates.

    abstract:CONTEXT:Uncaria tomentosa D.C. (Rubiaceae) has several biological activities, including activity against resistant Candida strains. The synergistic interaction with terbinafine or fluconazole can be an important alternative to overcome this resistance. OBJECTIVES:The potential synergy between a water insoluble fractio...

    journal_title:Pharmaceutical biology

    pub_type: 杂志文章

    doi:10.1080/13880209.2016.1242631

    authors: Moraes RC,Carvalho AR,Lana AJ,Kaiser S,Pippi B,Fuentefria AM,Ortega GG

    更新日期:2017-12-01 00:00:00

  • Hepatoprotective activity of Glycosmis pentaphylla against paracetamol-induced hepatotoxicity in Swiss albino mice.

    abstract:CONTEXT:Glycosmis pentaphylla (Retz.) DC (Rutaceae) is a shrub, traditionally used to treat anemia, rheumatism, as an anthelmintic, febrifuge and vermifuge, for jaundice, and liver complaints. OBJECTIVE:This study evaluated the hepatoprotective activity of G. pentaphylla against paracetamol-induced hepatotoxicity in S...

    journal_title:Pharmaceutical biology

    pub_type: 杂志文章

    doi:10.3109/13880209.2010.501084

    authors: Nayak SS,Jain R,Sahoo AK

    更新日期:2011-02-01 00:00:00

  • UHPLC/QTOF-MS-based metabolomics reveal the effect of Melastoma dodecandrum extract in type 2 diabetic rats.

    abstract::Context:Melastoma dodecandrum Lour. (Melastomataceae) is a traditional Chinese medicine. This is the first study to report a protective effect of the ethanol extract from M. dodecandrum (MDE) in type 2 diabetic (T2DM) rats.Objective: To investigate the therapeutic mechanism of MDE in T2DM rats.Materials and methods: S...

    journal_title:Pharmaceutical biology

    pub_type: 杂志文章

    doi:10.1080/13880209.2019.1693605

    authors: Weng J,Zhou J,Liang L,Li L

    更新日期:2019-12-01 00:00:00

  • Hepatoprotection of emodin and Polygonum multiflorum against CCl(4)-induced liver injury.

    abstract:CONTEXT:Polygonum multiflorum is known as a medicinal plant. It has been used as a folk medicine which showed antioxidative property. OBJECTIVE:Protective effects of the water extracts (w/v:1/10) from fresh P. multiflorum (WEP) on carbon tetrachloride (CCl(4))-induced liver damage in rats were investigated. MATERIALS...

    journal_title:Pharmaceutical biology

    pub_type: 杂志文章

    doi:10.3109/13880209.2011.604335

    authors: Lee BH,Huang YY,Duh PD,Wu SC

    更新日期:2012-03-01 00:00:00

  • Red ginseng powder fermented with probiotics exerts antidiabetic effects in the streptozotocin-induced mouse diabetes model.

    abstract:CONTEXT:Red ginseng (heat-processed Panax ginseng) is a well-known alternative medicine with pharmacological antidiabetic activity. It exerts pharmacological effects through the transformation of saponin into metabolites by the intestinal microbiota. Given that intestinal conditions and intestinal microflora vary among...

    journal_title:Pharmaceutical biology

    pub_type: 杂志文章

    doi:10.1080/13880209.2016.1237978

    authors: Jang SH,Park J,Kim SH,Choi KM,Ko ES,Cha JD,Lee YR,Jang H,Jang YS

    更新日期:2017-12-01 00:00:00

  • Lycopene attenuates endothelial dysfunction in streptozotocin-induced diabetic rats by reducing oxidative stress.

    abstract:CONTEXT:Diabetes mellitus is characterized by oxidative stress, which in turn induces endothelial dysfunction. As a potent antioxidant compound, lycopene might rescue diabetic endothelial dysfunction by reducing oxidative stress. OBJECTIVE:The present study investigated whether lycopene could lower oxidative stress an...

    journal_title:Pharmaceutical biology

    pub_type: 杂志文章

    doi:10.3109/13880209.2011.574707

    authors: Zhu J,Wang CG,Xu YG

    更新日期:2011-11-01 00:00:00

  • Dicranopteris linearis extract inhibits the proliferation of human breast cancer cell line (MDA-MB-231) via induction of S-phase arrest and apoptosis.

    abstract:CONTEXT:Dicranopteris linearis (Burm.f.) Underw. (Gleicheniaceae) has been scientifically proven to exert various pharmacological activities. Nevertheless, its anti-proliferative potential has not been extensively investigated. OBJECTIVE:To investigate the anti-proliferative potential of D. linearis leaves and determi...

    journal_title:Pharmaceutical biology

    pub_type: 杂志文章

    doi:10.1080/13880209.2018.1495748

    authors: Baharuddin AA,Roosli RAJ,Zakaria ZA,Md Tohid SF

    更新日期:2018-12-01 00:00:00

  • Effect of cocoyam (Colocasia esculenta), unripe plantain (Musa paradisiaca) or their combination on glycated hemoglobin, lipogenic enzymes, and lipid metabolism of streptozotocin-induced diabetic rats.

    abstract:CONTEXT:The possibility of combining unripe plantain [Musa paradisiacae Linn (Plantaginaceae)] and cocoyam [Colocassia esculenta Linn (Araceae)] in the management of diabetes has not been investigated. OBJECTIVE:The objective of this study is to evaluate the antihyperglycemic and antihyperlipidemic actions of unripe p...

    journal_title:Pharmaceutical biology

    pub_type: 杂志文章

    doi:10.3109/13880209.2015.1016181

    authors: Eleazu CO,Eleazu KC,Iroaganachi MA

    更新日期:2016-01-01 00:00:00

  • A sensitive WST-8-based bioassay for PEGylated granulocyte colony stimulating factor using the NFS-60 cell line.

    abstract:CONTEXT:Granulocyte colony stimulating factor (G-CSF) has been commonly used to treat neutropenia caused by chemotherapy, radiotherapy, and organ transplants. Improved in vitro efficacy of G-CSF has already been observed by conjugating it to polyethylene glycol (PEG). OBJECTIVE:The in vivo bioassay using tetrazolium d...

    journal_title:Pharmaceutical biology

    pub_type: 杂志文章

    doi:10.3109/13880209.2014.943248

    authors: Tiwari K,Wavdhane M,Haque S,Govender T,Kruger HG,Mishra MK,Chandra R,Tiwari D

    更新日期:2015-06-01 00:00:00

  • Evaluation of cytotoxic activity of patriscabratine, tetracosane and various flavonoids isolated from the Bangladeshi medicinal plant Acrostichum aureum.

    abstract:CONTEXT:Acrostichum aureumL. (Pteridaceae), a mangrove fern, has been used as a Bangladeshi traditional medicine for a variety of diseases including peptic ulcer. OBJECTIVE:Isolation and structural elucidation of cytotoxic secondary metabolites from the methanol extract of the aerial parts of A. aureum. MATERIALS AND...

    journal_title:Pharmaceutical biology

    pub_type: 杂志文章

    doi:10.3109/13880209.2012.673628

    authors: Uddin SJ,Grice D,Tiralongo E

    更新日期:2012-10-01 00:00:00