Hymenolepis diminuta: metacestode-induced reduction in the synthesis of the yolk protein, vitellogenin, in the fat body of Tenebrio molitor.

Abstract:

:Vitellogenin synthesis by the fat body has been monitored using in vitro culture and immunoprecipitation. This system was found to be efficient for measuring vitellogenin production in both non-infected Tenebrio molitor and those infected with Hymenolepis diminuta. In fat bodies from infected beetles, vitellogenin production was decreased by up to 75% (day 24 post-infection) and, at all times investigated, vitellogenin synthesis was significantly below control levels (days 3-30 post-infection). Incubating fat bodies from control insects with isolated metacestodes indicated that this may be a direct effect by the parasite which is developmental stage-specific. Stage II, but not Stage III-IV, not heat-killed parasites could bring about this decrease in vitellogenin. In addition, these effects may be density dependent within the range of 2-20 parasites per fat body; only 2 metacestodes were necessary to cause a significant decrease. Since metacestodes do not take up vitellogenin, nor limit the amount of [14C]leucine available to the fat body for vitellogenin production, it is conceivable that the parasite produces a potent inhibitor of vitellogenin synthesis, or a molecule which induces cells within the fat body.

journal_name

Parasitology

journal_title

Parasitology

authors

Webb TJ,Hurd H

doi

10.1017/s0031182000066658

subject

Has Abstract

pub_date

1996-04-01 00:00:00

pages

429-36

eissn

0031-1820

issn

1469-8161

journal_volume

112 ( Pt 4)

pub_type

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