Abstract:
:A rapid, visually read, dot-ELISA developed for the detection and differentiation of trypanosome species in tsetse flies (Glossina spp.), was field tested alongside the standard fly dissection method on a range in south eastern Kenya. Of 104 G. pallidipes dissected, 2 were found to be infected with trypanosomes in their midguts. By the dissection method the infecting trypanosome species could not be identified, as both flies had no salivary gland infections. However, using the dot-ELISA, the 2 flies were shown to be infected with Trypanosoma congolense in their midguts. The midguts of an additional 6 (5.8%) of the 104 G. pallidipes tested positive for T. congolense int he dot-ELISA, even though no trypanosomes were seen on dissection. The infection rate for this fly species as determined using the dot-ELISA, therefore, was 7.7% for T. congolense in midgut infections compared to 1.9% identified by fly dissection. The salivary glands and mouthparts of the 6 additional tsetse flies identified by dot-ELISA were all negative as determined by the 2 techniques. None of 390 G. longipennis flies dissected and examined for trypanosomes in the midgut, salivary glands and mouthparts was shown, by this method, to be infected. Using the dot-ELISA, however, 17 (4.4%) of the flies tested positve for T. congolense in the midgut, whilst the salivary glands and mouthparts of the same flies were negative. Thus, the dot-ELISA appears to be more sensitive than the fly dissection method under field conditions. Moreover, the dot-ELISA can be performed in the field without electricity. It is simple to perform, and was not affected by high ambient temperatures (22-32 degrees C), or by contamination of reactants with dust.
journal_name
Parasitologyjournal_title
Parasitologyauthors
Bosompem KM,Masake RA,Assoku RK,Opiyo EA,Nantulya VMdoi
10.1017/s0031182000084778subject
Has Abstractpub_date
1996-02-01 00:00:00pages
205-11eissn
0031-1820issn
1469-8161journal_volume
112 ( Pt 2)pub_type
杂志文章相关文献
PARASITOLOGY文献大全abstract::As more facts emerge regarding the ways in which E. multilocularis-derived molecules trigger the host immune response and modulate the host-parasite interplay, it becomes possible to envisage how the parasite can survive and proliferate in its intermediate host, while in other hosts it dies out. Through effects on cel...
journal_title:Parasitology
pub_type: 杂志文章,评审
doi:10.1017/S0031182009991533
更新日期:2010-03-01 00:00:00
abstract::Plant cysteine proteinases (CPs) from Carica papaya kill parasitic and free-living nematodes in vitro by hydrolysis of the worm cuticle, a mechanism that is different to all commercially available synthetic anthelmintics. We have developed a cheap and effective, rapid-throughput Caenorhabditis elegans-based assay for ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182016002353
更新日期:2017-04-01 00:00:00
abstract::Schizont-infected red blood cells (SI-RBC) from Plasmodium berghei-infected mice contain between 2 and 10 times as much sialic acid as uninfected RBC from the same blood (99-550 micrograms/10(10) RBC versus 33-65 micrograms/10(10) RBC). Total RBC samples from infected animals containing up to 63% ring- and trophozoite...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000065434
更新日期:1986-06-01 00:00:00
abstract::A 30-h in vitro susceptibility test of Plasmodium falciparum wild isolates to artemisinin, chloroquine, sulfadoxine/pyrimethamine and mefloquine was performed in Kibaha, Tanzania. A sigmoid Emax model was fitted to all data for each isolate and drug combination. Artemisinin and mefloquine exhibited 100% growth inhibit...
journal_title:Parasitology
pub_type: 杂志文章
doi:
更新日期:1997-06-01 00:00:00
abstract::Urinary schistosomiasis is an important source of human morbidity in Msambweni, Kenya, where the intermediate host snail, Bulinus nasutus is found in ponds and water pools. In the past, aquatic habitats in the area have been studied separately; however, recent collections of B. nasutus snails and shells indicated that...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182006001594
更新日期:2007-05-01 00:00:00
abstract::An electron microscopical study was made on the development of Eimeria dispersa in the small intestine of the domestic turkey. Turkey poults, 10-14 days of age, were inoculated with oocysts and pieces of intestinal tissue were fixed at intervals between 3.5 and 114 h after inoculation. Sporozoites were occasionally se...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000051611
更新日期:1982-02-01 00:00:00
abstract::We estimate the incidence rate, serological conversion rate and basic case reproduction number (R0) of Leishmania infantum from a cohort study of 126 domestic dogs exposed to natural infection rates over 2 years on Marajó Island, Pará State, Brazil. The analysis includes new methods for (1) determining the number of s...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182097001200
更新日期:1997-08-01 00:00:00
abstract::Philasterides dicentrarchi is a histophagous ciliate causing systemic scuticociliatosis in cultured turbot. This study demonstrates that turbot which survive this disease have serum antibodies that recognize ciliary antigens of this ciliate in ELISA and immobilize/agglutinate the ciliate in vitro. Mouse sera raised ag...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182002002688
更新日期:2003-02-01 00:00:00
abstract::A new histozoic species of myxosporean was found to infect the gill filaments, gut, kidney and liver of the freshwater teleost Leporinus friderici, collected from the estuarine region of the Amazon, near the city of Belém, Brazil. The plasmodia show asynchronous development, at any one time composed of mature spores a...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182003002944
更新日期:2003-04-01 00:00:00
abstract::During the early stages of the primary Plasmodium chabaudi chabaudi AS parasitaemia in CBA/Ca mice this parasite invaded normocytes, but as the parasitaemia developed increasing numbers of parasites were seen within reticulocytes. During and just after peak parasitaemia, as further parasite replication was controlled,...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000058583
更新日期:1989-10-01 00:00:00
abstract::Toxocariasis is a common helminthic infection that has a worldwide distribution. However, data from Lebanon about the prevalence of this infection are non-existent. We conducted a Toxocara seroprevalence study with 150 subjects attending the outpatient clinics at the American University of Beirut Medical Center betwee...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182005009637
更新日期:2006-05-01 00:00:00
abstract::Nematophagous fungi are used in the biological control of the parasitic helminths of animals and plants. The association of ovicidal and predator nematophagous fungi may present a complementary and increased action on the biological control of helminths. Joint growth compatibility and predation tests were carried out ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S003118201900060X
更新日期:2019-09-01 00:00:00
abstract::The surface coat of the 2nd-stage juveniles (J2) of plant-parasitic nematodes is considered to be involved in interactions with microorganisms in the soil and rhizosphere, as well as with the host plant. Characterization of surface antigens might be important in the development of new nematode control strategies. In t...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182002001907
更新日期:2002-08-01 00:00:00
abstract::Eimerian oocysts were found in 98% of 422 faeces samples from goats in SE England. It was found that 74% of the samples contained thousands or tens of thousands of oocysts/g. Kids less than 1 year old had higher oocyst counts than yearling or adult goats. Nine species of Eimeria were identified, and 65% of the samples...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000064052
更新日期:1986-04-01 00:00:00
abstract::The fine structure of the oncosphere of Hymenolepis nana has been investigated by transmission and scanning electron microscopy, together with light microscope observations of JB-4 embedded material. The outer surface of the oncosphere is covered by an epithelial layer, termed the embryonic epithelium. Cell types pres...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s003118200006697x
更新日期:1981-06-01 00:00:00
abstract::In order to study the effects of acclimatization of Plasmodium in beta-thalassaemic mice, we used a mouse model of beta-thalassaemia (DBA/2J/beta-thal/beta-thal), similar to that observed in humans. We acclimatized 3 rodent malarias (P. berghei, P. chabaudi and P. yoelii) in DBA/2J and DBA/2J/beta-thal mice lines, by ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000065781
更新日期:1996-03-01 00:00:00
abstract::The effects of the phenothiazine, Stelazine, on Hymenolepis diminuta were investigated. The cestode was incubated for 10 min at 37 degrees C with 1 mM trifluoperazine, in the presence and absence of Ca2+. Assay of brush border enzymes showed that drug treatment lowered the activities of alkaline phosphatase, Ca2+-ATP'...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s003118200005352x
更新日期:1987-02-01 00:00:00
abstract::The efficiency of treatment of human infections with the unicellular eukaryotic pathogens such as fungi and protozoa remains deeply unsatisfactory. For example, the mortality rates from nosocomial fungemia in critically ill, immunosuppressed or post-cancer patients often exceed 50%. A set of six systemic clinical azol...
journal_title:Parasitology
pub_type: 杂志文章,评审
doi:10.1017/S0031182018000562
更新日期:2018-12-01 00:00:00
abstract::Schistosomula of Schistosoma mansoni were incubated in RPMI 1640 medium containing 10% fetal calf serum, 10% human portal venous or 10% human peripheral venous sera in the presence of bromodeoxyuridine (BrdU) in order to measure differences in cell proliferation. The rates of cell proliferation as expressed by BrdU la...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182098003096
更新日期:1998-10-01 00:00:00
abstract::An electron microscopical examination has been made of the fine structure and disposition of pancreatic polypeptide immunoreactive cells associated with the egg-forming apparatus in Diclidophora merlangi. The cell bodies are positioned in the parenchyma surrounding the ootype and taper to axon-like processes that exte...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000064404
更新日期:1991-06-01 00:00:00
abstract::Existing models of the basic case reproduction number (R0) for vector-borne diseases assume (i) that the distribution of vectors over the susceptible host species is homogeneous and (ii) that the biting preference for the susceptible host species rather than other potential hosts is a constant. Empirical evidence cont...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182099005442
更新日期:2000-03-01 00:00:00
abstract::A multi-subunit antigen (native M(r) > 200 kDa, reduced M(r) 97-100 kDa) has been identified in homogenates of Trichinella spiralis larvae using affinity-purified rabbit anti-keyhole limpet haemocyanin (KLH) antibodies and its cross-reactivity with KLH was confirmed by competition blotting. The antigen was not present...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000080793
更新日期:1994-11-01 00:00:00
abstract::Cystic echinococcosis (CE) is a high prevalence zoonosis among the rural population of Tacuarembó (Uruguay). The correlation between serological data and the incidence of risk factors was studied in a survey carried out in 1998 among rural communities where 480 individuals were examined by means of abdominal sonograph...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182004006717
更新日期:2005-04-01 00:00:00
abstract::A serine protease was purified 942-fold from culture supernatant of L. amazonensis promastigotes using (NH4)2SO4 precipitation followed by affinity chromatography on aprotinin-agarose and continuous elution electrophoresis by Prep Cell, yielding a total recovery of 61%. The molecular mass of the active enzyme estimate...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182004006675
更新日期:2005-07-01 00:00:00
abstract::Various treatments are found to be moderately effective in managing Demodex-related diseases except tea tree oil (TTO) and terpinen-4-ol (T4O), which showed superior miticidal and anti-inflammatory effects in numerous clinical studies. Their possible effects include lowering mite counts, relieving Demodex-related symp...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S003118202000150X
更新日期:2020-12-01 00:00:00
abstract::Chagas disease is one of the most important vector-borne zoonotic diseases in Latin America. Control strategies could be improved if transmissibility patterns of its aetiologic agent, Trypanosoma cruzi, were better understood. To understand transmissibility patterns of Chagas disease in Mexico, we inferred potential v...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182016002468
更新日期:2017-05-01 00:00:00
abstract::Nematodes are amongst the most successful and abundant organisms on the planet with approximately 30 000 species described, although the actual number of species is estimated to be one million or more. Despite sharing a relatively simple and invariant body plan, there is considerable diversity within the phylum. Nemat...
journal_title:Parasitology
pub_type: 杂志文章,评审
doi:10.1017/S0031182014000304
更新日期:2014-08-01 00:00:00
abstract::Toxoplasma gondii and Sarcocystis neurona are protozoan parasites with terrestrial definitive hosts, and both pathogens can cause fatal disease in a wide range of marine animals. Close monitoring of threatened southern sea otters (Enhydra lutris nereis) in California allowed for the diagnosis of dual transplacental tr...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182015001377
更新日期:2016-03-01 00:00:00
abstract::Aiming to further characterize the haemocyte subsets in Biomphalaria snails, we have performed a detailed flow cytometric analysis of whole haemolymph cellular components using a multiparametric dual colour labelling procedure. Ethidium bromide/acridine orange fluorescence features were used to first select viable hae...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182008005155
更新日期:2009-01-01 00:00:00
abstract::This review of protozoan and helminth infections in pregnancy focuses on the impact on the immune response in the newborn infant to maternal infection. Studies of protozoan and helminth infections in pregnant women and in their offspring have shown that children exposed to antigens or microorganisms during pregnancy o...
journal_title:Parasitology
pub_type: 杂志文章,评审
doi:10.1017/S0031182007000182
更新日期:2007-01-01 00:00:00