Abstract:
:A rapid, visually read, dot-ELISA developed for the detection and differentiation of trypanosome species in tsetse flies (Glossina spp.), was field tested alongside the standard fly dissection method on a range in south eastern Kenya. Of 104 G. pallidipes dissected, 2 were found to be infected with trypanosomes in their midguts. By the dissection method the infecting trypanosome species could not be identified, as both flies had no salivary gland infections. However, using the dot-ELISA, the 2 flies were shown to be infected with Trypanosoma congolense in their midguts. The midguts of an additional 6 (5.8%) of the 104 G. pallidipes tested positive for T. congolense int he dot-ELISA, even though no trypanosomes were seen on dissection. The infection rate for this fly species as determined using the dot-ELISA, therefore, was 7.7% for T. congolense in midgut infections compared to 1.9% identified by fly dissection. The salivary glands and mouthparts of the 6 additional tsetse flies identified by dot-ELISA were all negative as determined by the 2 techniques. None of 390 G. longipennis flies dissected and examined for trypanosomes in the midgut, salivary glands and mouthparts was shown, by this method, to be infected. Using the dot-ELISA, however, 17 (4.4%) of the flies tested positve for T. congolense in the midgut, whilst the salivary glands and mouthparts of the same flies were negative. Thus, the dot-ELISA appears to be more sensitive than the fly dissection method under field conditions. Moreover, the dot-ELISA can be performed in the field without electricity. It is simple to perform, and was not affected by high ambient temperatures (22-32 degrees C), or by contamination of reactants with dust.
journal_name
Parasitologyjournal_title
Parasitologyauthors
Bosompem KM,Masake RA,Assoku RK,Opiyo EA,Nantulya VMdoi
10.1017/s0031182000084778subject
Has Abstractpub_date
1996-02-01 00:00:00pages
205-11eissn
0031-1820issn
1469-8161journal_volume
112 ( Pt 2)pub_type
杂志文章相关文献
PARASITOLOGY文献大全abstract::Endotoxaemia has been described in cases of Human African trypanosomiasis (HAT), but it is unclear if this phenomenon influences inflammatory pathology either in the periphery or central nervous system (CNS). We studied endotoxin concentrations in the plasma and cerebrospinal fluid (CSF) of Trypanosoma brucei rhodesie...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182016002158
更新日期:2017-04-01 00:00:00
abstract::To investigate the quantitative distribution of Plasmodiumfalciparum gametocytes into the vector bloodmeal, Anopheles arabiensis mosquitoes were fed on 3 volunteers naturally infected with gametocytes. The content of each mosquito midgut was smeared on a microscope slide and Giemsa stained. The distribution of gametoc...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182099006277
更新日期:2000-08-01 00:00:00
abstract::Due to the low informative value of available morphological characters, cytogenetic and molecular methods, based on rDNA sequencing, were used to characterize adult and larval stages of Phyllodistomum spp. Species studied have 18 chromosomes with comparable absolute and relative lengths. Conventional Giemsa staining a...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182004006109
更新日期:2004-12-01 00:00:00
abstract::Toxocariasis is a common helminthic infection that has a worldwide distribution. However, data from Lebanon about the prevalence of this infection are non-existent. We conducted a Toxocara seroprevalence study with 150 subjects attending the outpatient clinics at the American University of Beirut Medical Center betwee...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182005009637
更新日期:2006-05-01 00:00:00
abstract::The induction of schistosomulicidal activity of peritoneal macrophages by concanavalin A-stimulated supernatants from long-term T-cell clones and by interferon-gamma (IFN-gamma) was investigated in detail. Optimal conditions of in vitro macrophage activation by T-cell clone supernatants were established. Macrophages f...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s003118200006409x
更新日期:1986-04-01 00:00:00
abstract::The blackcap (Sylvia atricapilla) is a common Palearctic migratory warbler, and haemosporidian parasites are common in this species. However, genetic and phenotypic diversity of haemosporidians in warblers has been insufficiently investigated and poorly linked. We addressed this issue by combining molecular and micros...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182009991235
更新日期:2010-02-01 00:00:00
abstract::Host-parasite interactions are shaped by the co-evolutionary arms race of parasite virulence, transmission success as well as host resistance and recovery. The virulence and fitness of parasites may depend on host condition, which is mediated, for instance, by host energy constraints. Here, we investigated to what ext...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182010000995
更新日期:2011-01-01 00:00:00
abstract::Common bird species were screened during May and June 2007 on Barbados for haemosporidian parasites (Haemosporida) of the genera Haemoproteus and Plasmodium to determine whether the low parasite diversity reported in previous studies might have reflected limited sampling. PCR screening and DNA sequencing revealed a si...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182009990497
更新日期:2009-09-01 00:00:00
abstract::Invasive parasites can spill over to new hosts in invaded ecosystems with often unpredictable trophic relationships in the newly arising parasite-host interactions. In European seas, the intestinal copepod Mytilicola orientalis was co-introduced with Pacific oysters (Magallana gigas) and spilled over to native blue mu...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182017001779
更新日期:2018-05-01 00:00:00
abstract::An hypothesis is presented to explain the red cell lysis which accompanies an acute malarial infection, as well as the mode of action of certain schizonticidal drugs in the quinoline and acridine series. Quinine and a number of other antimalarial drugs have been found to counteract the inhibition by protein of fatty a...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s003118200004662x
更新日期:1975-10-01 00:00:00
abstract::Intestinal diarrheagenic polyparasitic infections are among the major public health concerns in developing countries. Here we examined stool specimens by microscopy, DNA dot blot and polymerase chain reaction (PCR) to evaluate the co-infection of four principal protozoans among amoebic dysentery cases from Northeast I...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182015000669
更新日期:2015-09-01 00:00:00
abstract::Dr David Livingstone died on May 1st 1873. He was 60 years old and had spent much of the previous 30 years walking across large stretches of Southern Africa, exploring the terrain he hoped could provide new environments in which Europeans and Africans could cohabit on equal terms and bring prosperity to a part of the ...
journal_title:Parasitology
pub_type: 传,历史文章,杂志文章
doi:10.1017/S003118201600202X
更新日期:2017-10-01 00:00:00
abstract::Understanding the origin of invasive parasites and ecological transmission barriers on the distribution of mosquito-borne pathogens is enriched by molecular phylogenetic approaches now that large databases are becoming available. Here we assess the biogeographical relationships among haemosporidian blood parasites and...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182011001491
更新日期:2011-12-01 00:00:00
abstract::Oesophagostomum dentatum stages were investigated for glutathione S-transferase (GST) expression at the protein and mRNA levels. GST activity was detected in all stages (infectious and parasitic stages including third- and fourth-stage larvae of different ages as well as males and females) and could be dose-dependentl...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182008004769
更新日期:2008-09-01 00:00:00
abstract::A striking feature of toxoplasmic seroconversion is the prominent and early IgM response to a low molecular weight antigen of 4-5 kDa. Two different monoclonal antibodies directed against the 4-5 kDa antigen have been generated and used to characterize this molecule. Using these monoclonal antibodies, we could demonst...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000068220
更新日期:1994-02-01 00:00:00
abstract::A multi-subunit antigen (native M(r) > 200 kDa, reduced M(r) 97-100 kDa) has been identified in homogenates of Trichinella spiralis larvae using affinity-purified rabbit anti-keyhole limpet haemocyanin (KLH) antibodies and its cross-reactivity with KLH was confirmed by competition blotting. The antigen was not present...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000080793
更新日期:1994-11-01 00:00:00
abstract::The complete small subunit ribosomal RNA (srRNA) gene of Theileria parva was cloned and sequenced. Two primers were designed which permitted the specific amplification of part of the Theileria srRNA gene from Theileria-infected cell line samples which were predominantly (> 95%) bovine DNA. The sequence of the central ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000067263
更新日期:1993-08-01 00:00:00
abstract::In an interconnected world, the international pet trade on wild animals is becoming increasingly important. As a consequence, non-native parasite species are introduced, which affect the health of wildlife and contribute to the loss of biodiversity. Because the investigation of parasite diversity within vulnerable hos...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182016002183
更新日期:2017-04-01 00:00:00
abstract::A serine protease was purified 942-fold from culture supernatant of L. amazonensis promastigotes using (NH4)2SO4 precipitation followed by affinity chromatography on aprotinin-agarose and continuous elution electrophoresis by Prep Cell, yielding a total recovery of 61%. The molecular mass of the active enzyme estimate...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182004006675
更新日期:2005-07-01 00:00:00
abstract::The localization of adult Schistosoma mansoni originating from Guadeloupe (West Indies) was analysed in the natural host Rattus rattus in experimental and natural infections. A transfer of schistosomes from the porto-mesenteric system to the lungs occurs between the 4th and the 20th weeks post-infection, with a peak b...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000060790
更新日期:1992-02-01 00:00:00
abstract::Canine leishmaniosis (CanL) is caused by the parasite Leishmania infantum and is a systemic disease, which can present with variable clinical signs, and clinicopathological abnormalities. Clinical manifestations can range from subclinical infection to very severe systemic disease. Leishmaniosis is categorized as a neg...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S003118201600055X
更新日期:2017-01-01 00:00:00
abstract::Endosymbiotic bacteria of the genus Wolbachia infect a number of invertebrate species in which they induce various alterations in host reproduction, mainly cytoplasmic incompatibility (CI). In contrast to most other maternally transmitted parasites, manipulation of host reproduction makes the spread of Wolbachia possi...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182099006599
更新日期:2000-11-01 00:00:00
abstract::The period 1875-1925 was remarkable in the history of parasitology mainly for the elucidation of the life cycles of parasites causing important parasitic diseases and the incrimination of vectors in their transmission. These discoveries were made by a small number of scientists working in the tropics a number of whom ...
journal_title:Parasitology
pub_type: 社论,历史文章
doi:10.1017/S0031182017000956
更新日期:2017-10-01 00:00:00
abstract::Many different drug-resistant lines of rodent malaria are available as screening models. It is obligatory to screen new compounds for antimalarial activity against a series of resistant lines in order to identify a compound with potential for the treatment of multi-drug resistant (MDR) malaria infections. Instead of u...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s003118200500778x
更新日期:2005-09-01 00:00:00
abstract::Receptors interacting with terminal sugars as ligands are involved in the binding of Leishmania donovani promastigotes to the macrophage surface and their subsequent internalization. Mannose and glucose are specifically involved in the binding process. Decreased binding occurs to macrophages already infected with L. d...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000076253
更新日期:1994-08-01 00:00:00
abstract::Several approaches were adopted for the disruption and removal of the tegumental surface from protoscoleces of the horse strain of the hydatid organism, Echinococcus granulosus. The effectiveness of each method and the purity of subsequent microthrix-enriched fractions obtained by differential centrifugation were eval...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000049064
更新日期:1985-02-01 00:00:00
abstract::We examined the effects of Haemoproteus infection on the survival and pathology caused in the biting midges. Forty-six females of Culicoides impunctatus were exposed experimentally by allowing them to feed on a naturally infected red-backed shrike infected with Haemoproteus lanii (lineage hRB1, gametocytaemia 5·2%). S...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182016001426
更新日期:2016-11-01 00:00:00
abstract::We studied the pattern of infection and the inter-annual variation and individual factors affecting the infection of 2 species of nasopharyngeal bot flies, Cephenemyia auribarbis and Pharyngomyia picta (Diptera: Oestridae), in a population of Iberian red deer (Cervus elaphus hispanicus) from south central Spain (10 an...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182004005578
更新日期:2004-09-01 00:00:00
abstract::An immunochromatographic test (ICT), using recombinant truncated P50 (P50t), for the detection of antibodies to Babesia gibsoni was developed and evaluated. Whereas all sera from specific pathogen-free dogs were clearly negative, all sera from dogs experimentally infected with B. gibsoni were clearly positive in the I...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182005008401
更新日期:2005-12-01 00:00:00
abstract::The World Health Organization announced in November 2014 at the fourth international meeting on 'the control of neglected zoonotic diseases - from advocacy to action', that intervention tools for eliminating Taenia solium taeniosis/cysticercosis (TSTC) are in place. The aim of this work was to elucidate theoretical ou...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182016000500
更新日期:2017-01-01 00:00:00