Abstract:
:Schizont-infected red blood cells (SI-RBC) from Plasmodium berghei-infected mice contain between 2 and 10 times as much sialic acid as uninfected RBC from the same blood (99-550 micrograms/10(10) RBC versus 33-65 micrograms/10(10) RBC). Total RBC samples from infected animals containing up to 63% ring- and trophozoite-infected cells had identical sialic acid contents to purified RBC samples (of less than 3% parasitaemia) from the same blood (52-64 micrograms/10(10) RBC). We conclude that RBC containing immature parasites have the same sialic acid content as uninfected RBC from infected blood and that total cellular sialic acid increases during maturation to the schizont stage. Uninfected RBC from infected blood had 25-50% as much sialic acid as normal mouse RBC (33-65 micrograms/10(10) RBC versus 126 micrograms/10(10) RBC). There were no qualitative changes in RBC sialic acids, all RBC samples having 60-70% N-acetylneuraminic acid, 30-40% N-acetyl-9-O-acetylneuraminic acid and 5-10% N-glycolylneuraminic acid. The quantitative changes we observed during infection must reflect changes in murine sialoglycoconjugates, as we have shown elsewhere that Plasmodia do not synthesize or contain sialic acids. Since the sialic acid composition of mouse serum glycoconjugates is quite different to that of the RBC fractions studied here, the quantitative data suggest that part of the sialic acids of the uninfected RBC has been transferred to SI-RBC. With higher molar ratios of periodate to substrate than generally used, we were able to radio-isotopically label normal murine sialoglycoproteins on SI-RBC and purified uninfected RBC from infected blood by the periodate/NaB3H4 method. Several new proteins were then tritiated with SI-RBC but these proteins may be intracellular and could even lack sialic acid.
journal_name
Parasitologyjournal_title
Parasitologyauthors
Howard RJ,Seeley DC Jr,Kao V,Wember M,Schauer Rdoi
10.1017/s0031182000065434subject
Has Abstractpub_date
1986-06-01 00:00:00pages
545-57eissn
0031-1820issn
1469-8161journal_volume
92 ( Pt 3)pub_type
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