Abstract:
:Schistosomula of Schistosoma mansoni were incubated in RPMI 1640 medium containing 10% fetal calf serum, 10% human portal venous or 10% human peripheral venous sera in the presence of bromodeoxyuridine (BrdU) in order to measure differences in cell proliferation. The rates of cell proliferation as expressed by BrdU labelling indices (BLI) were determined as a function of time of incubation by immunohistochemistry using monoclonal antibody to BrdU. Compared to schistosomula cultured in the presence of RPMI plus 10% fetal calf serum, BLIs were increased by 60% in the presence of human portal, but not in peripheral serum. This stimulatory effect was substantially reproduced by a fraction of portal serum with a molecular weight range between 1 and 50 kDa. However, in the presence of human peripheral venous serum, either whole or fractionated, schistosomula showed no significant difference compared to RPMI plus 10% fetal calf serum alone. Furthermore, human portal serum fractions of molecular weight greater than 50 kDa also revealed no significant difference relative to control. The results indicate that portal venous serum component(s) of a molecular weight range higher than most simple nutrients can greatly stimulate the rate of cell proliferation of Schistosoma mansoni schistosomula.
journal_name
Parasitologyjournal_title
Parasitologyauthors
Shaker YM,Wu CH,el-Shobaki FA,Ashour E,Khattab HM,Draz HM,Kamel R,Wu GYdoi
10.1017/s0031182098003096subject
Has Abstractpub_date
1998-10-01 00:00:00pages
293-9eissn
0031-1820issn
1469-8161journal_volume
117 ( Pt 4)pub_type
杂志文章相关文献
PARASITOLOGY文献大全abstract::Recent publications demonstrated that a fragment of a Neospora caninum ROP2 family member antigen represents a promising vaccine candidate. We here report on the cloning of the cDNA encoding this protein, N. caninum ROP2 family member 1 (NcROP2Fam-1), its molecular characterization and localization. The protein posses...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182013000383
更新日期:2013-07-01 00:00:00
abstract::Conventional mice and congenitally athymic, nude mice were infected with 20 metacercariae of the intestinal trematode Echinostoma revolutum. The sequential events in the pathological changes in the intestine were studied at different intervals post-infection. By day 11 onwards the conventional mice displayed dilatatio...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000054706
更新日期:1984-06-01 00:00:00
abstract::Human cysticercosis is a public health problem caused by Taenia solium metacestodes; thus, eradication of T. solium transmission by vaccination is an urgent requirement. The Cc48 mimotope from T. solium cysticerci was tested expressed in phage particles (mCc48) and chemically synthesized (sCc48) as a vaccine candidate...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182020001080
更新日期:2020-10-01 00:00:00
abstract::Voltage-gated calcium (Ca2+) channels contribute to impulse propagation in excitable cells and also regulate intracellular levels of Ca2+. High voltage-activated (HVA) Ca2+ channels are heteromultimeric membrane proteins. The pore-forming, voltage-sensing subunit is the alpha1 subunit. We have cloned 3 HVA Ca2+ channe...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182001008691
更新日期:2001-11-01 00:00:00
abstract::To test the homogeneity of 18S sequences within Trypanosoma cruzi, riboprint profiles were separated by temperature gradient gel electrophoresis (TGGE). Upon interpretation of melting curves of fragments within a riboprint profile, there appeared to be two 18S sequence types within each stock examined. Two similar typ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182098002972
更新日期:1998-09-01 00:00:00
abstract::Cod (Gadus morhua), an important fish species in the Baltic Sea, is the paratenic host for many parasite species, including the zoonotic nematodes, Anisakis sp. and Contracaecum osculatum. We aimed to identify which invertebrate species (found in situ in the fish stomach) are responsible for infection of cod with zoon...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182019001124
更新日期:2019-11-01 00:00:00
abstract::The mechanism by which Giardia lamblia exerts its pathogenicity is likely to be multifactorial. A 58 kDa enterotoxin was purified and characterized from the excretory-secretory product (ESP) of the parasite (Kaur et al. 2001). In the present study an attempt has been made to elucidate the mechanism of action of the ES...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182004005852
更新日期:2004-10-01 00:00:00
abstract::Past and more recent research has examined the ultrastructure, metabolism, cell biology, genomics and post-genomics of schistosome schistosomula. These areas are considered and discussed in this review with particular emphasis on (1) the early migration phases through the host, (2) interaction of the host immune respo...
journal_title:Parasitology
pub_type: 杂志文章,评审
doi:10.1017/S0031182006001648
更新日期:2007-04-01 00:00:00
abstract::Lactating and nulliparous outbred Swiss (CF-1 strain) mice were infected at 12-16 weeks of age with Nippostrongylus brasiliensis or Trichinella spiralis. Lysophospholipase B levels in the intestinal tissue and faecal pellets were greatly in infected lactating mice in contrast to infected nulliparous mice. Correlated w...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000054998
更新日期:1980-08-01 00:00:00
abstract::Parasitoids are parasites that invariably kill their host. Polydnaviruses are injected by parasitoid wasps into the body cavity of their insect host and cause immunosuppression, allowing the parasitoid to develop in the absence of encapsulation. One of the targets of the polydnaviruses are the haemocytes of the host, ...
journal_title:Parasitology
pub_type: 杂志文章,评审
doi:10.1017/s0031182000084948
更新日期:1998-01-01 00:00:00
abstract::Uptake of radioactivity labelled purine bases and nucleosides by suspensions of Trypanosoma brucei and Trypanosoma congolense in bicine buffer was determined at 37 degrees C. With T. brucei, the rate of uptake of adenosine was much greater than that of the other compounds tested, the uptake of which decreased in the o...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000056110
更新日期:1980-10-01 00:00:00
abstract::Syphacia stroma (von Linstow, 1884) Morgan, 1932 and Syphacia frederici Roman, 1945 are oxyurid nematodes that parasitize two murid rodents, Apodemus sylvaticus and Apodemus flavicollis, on the European mainland. Only S. stroma has been recorded previously in Apodemus spp. from the British Isles. Despite the paucity o...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182017001470
更新日期:2018-03-01 00:00:00
abstract::Circumstantial evidence suggests that the earlier detachment of Boophilus microplus larvae from highly resistant cattle follows the release of histamine at the attachment site. In vivo and in vitro experiments show that a proportion of the larvae will detach following injection or infusion of histamine. Other mediator...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000000950
更新日期:1980-06-01 00:00:00
abstract::The transmission success of Onchocerca volvulus is thought to be influenced by a variety of regulatory or density-dependent processes that act at various points in the two-host life-cycle. This paper examines one component of the life-cycle, namely, the ingestion of microfilariae by the simuliid vector, to assess the ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000078586
更新日期:1994-01-01 00:00:00
abstract::Invasive parasites can spill over to new hosts in invaded ecosystems with often unpredictable trophic relationships in the newly arising parasite-host interactions. In European seas, the intestinal copepod Mytilicola orientalis was co-introduced with Pacific oysters (Magallana gigas) and spilled over to native blue mu...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182017001779
更新日期:2018-05-01 00:00:00
abstract::The recent development of genetic methods allows the delineation of species boundaries, especially in organisms where morphological characters are not reliable to differentiate species. However, few empirical studies have used these tools to delineate species among parasitic metazoans. Here we investigate the species ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182016001517
更新日期:2016-11-01 00:00:00
abstract::A striking feature of toxoplasmic seroconversion is the prominent and early IgM response to a low molecular weight antigen of 4-5 kDa. Two different monoclonal antibodies directed against the 4-5 kDa antigen have been generated and used to characterize this molecule. Using these monoclonal antibodies, we could demonst...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000068220
更新日期:1994-02-01 00:00:00
abstract::Previous studies of mating interactions between schistosome species from different evolutionary lineages in mice showed that mating is not random: heterospecific pairs form but, given the choice, the preference is for homospecific partners. Mating competition and change of mate were also demonstrated. Schistosoma mans...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182002002111
更新日期:2002-09-01 00:00:00
abstract::Trichomonas vaginalis induces cellular damage to the host cells (cytotoxicity) through the proteolytic activity of multiple proteinases of the cysteine type (CPs). Some CPs are modulated by environmental factors such as iron, zinc, polyamines, etc. Thus, the goal of this study was to assess the effect of glucose on T....
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182019000209
更新日期:2019-08-01 00:00:00
abstract::Schistosomiasis is a health problem in Brazil and the role of rodents in maintaining the schistosome life-cycle requires further clarification. The influence of Schistosoma mansoni on a population of Nectomys squamipes was studied by capture-recapture (1st phase, from June 1991 to November 1995) and removal (2nd phase...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182099005892
更新日期:2000-06-01 00:00:00
abstract::The diversity and prevalence of malaria parasites of the genera Plasmodium and Haemoproteus were determined in the globally-threatened Aquatic Warbler Acrocephalus paludicola. Birds were sampled during migration in Portugal and at the wintering quarters in Senegal and parasites were detected using molecular methods. O...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182015000414
更新日期:2015-08-01 00:00:00
abstract::In this review, we envisage the host environment, not as a hostile one, since the schistosome thrives there, but as one in which the relationship between the two organisms consists of constant communication, through signalling mechanisms involving sense organs, surface glycocalyx, surface membrane and internal organs ...
journal_title:Parasitology
pub_type: 杂志文章,评审
doi:10.1017/S0031182007002971
更新日期:2007-10-01 00:00:00
abstract::To compare the structures of natural and re-established populations of Ascaris in humans, universal (mass) chemotherapy was carried out at the beginning and the end of the study year using pyrantel pamoate. Worms expelled within 48 h of treatment were collected, their sex determined, and measurements made of length, w...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182002001737
更新日期:2002-06-01 00:00:00
abstract::Protective immunity against larval Taenia crassiceps has been shown to rely on T cells; however, the roles of the specific subsets of T cells during infection are not known. To investigate a possible role for gamma delta T cells, this study investigated larval infection in delta-chain knock-out C57BL/6 (deltaKO) and w...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182099004771
更新日期:1999-10-01 00:00:00
abstract::This cross-sectional study aimed to determine the prevalence and risk factors of S. stercoralis infection among 1142 Orang Asli primary schoolchildren in six different states of Peninsular Malaysia. Fecal samples were examined using direct smear, formalin-ether sedimentation (FES), agar plate culture (APC) and PCR tec...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182019000945
更新日期:2019-10-01 00:00:00
abstract::Glycans at the surface of adult Schistosoma mansoni were investigated with gold-labelled lectins. The fragile complex of the glycans with the outer membranes could be preserved for electron microscopy by avoiding extensive pre-fixation with aldehydes and by introducing osmium-ferrocyanide as a membrane fixative. Male ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000081877
更新日期:1995-09-01 00:00:00
abstract::Sera from goats and cattle that were infected with two Trypanosoma vivax clones (ILDat 1.2 and ILDat 2.1) derived from different stocks were analysed for antibody activity against the variable surface glycoproteins (VSGs) of the infecting clones by enzyme-linked immune assays (ELISA) and immune lysis. To obtain purifi...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000060157
更新日期:1990-02-01 00:00:00
abstract::Human strongyloidiasis is a serious disease mostly attributable to Strongyloides stercoralis and to a lesser extent Strongyloides fuelleborni, a parasite mainly of non-human primates. The role of animals as reservoirs of human-infecting Strongyloides is ill-defined, and whether dogs are a source of human infection is ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182020000979
更新日期:2020-09-01 00:00:00
abstract::In previous studies, ultrastructural observations revealed a large number of gap junctions (GJs) in the neck and immature proglottid tissues of Taenia solium tapeworms. In these helminths, cytoplasmic glycogen sacs are connected by numerous discrete GJs to other cells throughout the maturing strobilar tissue. Disconti...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182008004629
更新日期:2008-08-01 00:00:00
abstract::Changes during development in the elemental contents (Ca, P, K, Mg and S) of male and female Schistosoma mansoni from both mixed and single sex infections have been measured for the first time. In mixed infections, there is a significant difference between the calcium content of male and female worms; female worms hav...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000050630
更新日期:1983-06-01 00:00:00