Glucose-restriction increases Trichomonas vaginalis cellular damage towards HeLa cells and proteolytic activity of cysteine proteinases (CPs), such as TvCP2.

Abstract:

:Trichomonas vaginalis induces cellular damage to the host cells (cytotoxicity) through the proteolytic activity of multiple proteinases of the cysteine type (CPs). Some CPs are modulated by environmental factors such as iron, zinc, polyamines, etc. Thus, the goal of this study was to assess the effect of glucose on T. vaginalis cytotoxicity, proteolytic activity and the particular role of TvCP2 (TVAG_057000) during cellular damage. Cytotoxicity assays showed that glucose-restriction (GR) promotes the highest HeLa cell monolayers destruction (~95%) by trichomonads compared to those grown under high glucose (~44%) condition. Zymography and Western blot using different primary antibodies showed that GR increased the proteolytic activity, amount and secretion of certain CPs, including TvCP2. We further characterized the effect of glucose on TvCP2. TvCP2 increases in GR, localized in vesicles close to the plasma membrane and on the surface of T. vaginalis. Furthermore, pretreatment of GR-trichomonads with an anti-TvCP2r polyclonal antibody specifically reduced the levels of cytotoxicity and apoptosis induction to HeLa cells in a concentration-dependent manner. In conclusion, our data show that GR, as a nutritional stress condition, promotes trichomonal cytotoxicity to the host cells, increases trichomonad proteolytic activity and amount of CPs, such as TvCP2 involved in cellular damage.

journal_name

Parasitology

journal_title

Parasitology

authors

Miranda-Ozuna JFT,Rivera-Rivas LA,Cárdenas-Guerra RE,Hernández-García MS,Rodríguez-Cruz S,González-Robles A,Chavez-Munguía B,Arroyo R

doi

10.1017/S0031182019000209

subject

Has Abstract

pub_date

2019-08-01 00:00:00

pages

1156-1166

issue

9

eissn

0031-1820

issn

1469-8161

pii

S0031182019000209

journal_volume

146

pub_type

杂志文章
  • Isolation and genetic characterization of Neospora caninum from asymptomatic calves in Spain.

    abstract::Neospora caninum is a cyst-forming parasite that causes abortion in cattle. Despite this parasite's ubiquitous distribution and wide host range, the number of N. caninum isolates obtained to date is limited. In vitro isolation of the parasite is arduous and often unsuccessful. In addition, most isolates have been obta...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S003118200800509X

    authors: Regidor-Cerrillo J,Gómez-Bautista M,Pereira-Bueno J,Aduriz G,Navarro-Lozano V,Risco-Castillo V,Férnandez-García A,Pedraza-Díaz S,Ortega-Mora LM

    更新日期:2008-12-01 00:00:00

  • Machine learning-based analyses support the existence of species complexes for Strongyloides fuelleborni and Strongyloides stercoralis.

    abstract::Human strongyloidiasis is a serious disease mostly attributable to Strongyloides stercoralis and to a lesser extent Strongyloides fuelleborni, a parasite mainly of non-human primates. The role of animals as reservoirs of human-infecting Strongyloides is ill-defined, and whether dogs are a source of human infection is ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182020000979

    authors: Barratt JLN,Sapp SGH

    更新日期:2020-09-01 00:00:00

  • Differentially expressed parasite genes involved in host recognition and invasion of the triactinomyxon stage of Myxobolus cerebralis (Myxozoa).

    abstract::The host recognition and invasion process of Myxobolus cerebralis actinospores (triactinomyxon, TAM) was studied on a genetic level. A small-scale in vitro assay was developed to activate a large number of TAMs simultaneously, and to monitor the host invasion in the absence of live fish. The transcriptomes of non-acti...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182008005398

    authors: Eszterbauer E,Kallert DM,Grabner D,El-Matbouli M

    更新日期:2009-04-01 00:00:00

  • Trophic relationship between the invasive parasitic copepod Mytilicola orientalis and its native blue mussel (Mytilus edulis) host.

    abstract::Invasive parasites can spill over to new hosts in invaded ecosystems with often unpredictable trophic relationships in the newly arising parasite-host interactions. In European seas, the intestinal copepod Mytilicola orientalis was co-introduced with Pacific oysters (Magallana gigas) and spilled over to native blue mu...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182017001779

    authors: Goedknegt MA,Shoesmith D,Jung AS,Luttikhuizen PC,van der Meer J,Philippart CJM,van der Veer HW,Thieltges DW

    更新日期:2018-05-01 00:00:00

  • Immunochemical analysis of surface membrane antigens on erythrocytes infected with non-cloned SICA[+] or cloned SICA[-] Plasmodium knowlesi.

    abstract::The SICA[-] or non-agglutinable phenotype of Plasmodium knowlesi schizont-infected erythrocytes has been defined serologically but not biochemically. Similarly, non-cloned SICA[+] or agglutinable parasites have been shown serologically to express SICA or variant antigen(s) but the number and nature of such antigens ha...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000057346

    authors: Howard RJ,Barnwell JW

    更新日期:1985-10-01 00:00:00

  • Immune-dependent thrombocytopaenia in mice infected with Schistosoma mansoni.

    abstract::As has been shown previously, immunologically intact mice with patent Schistosoma mansoni infections had a significantly lower mean platelet number than intact uninfected mice (P<0.0001). However, platelet numbers in T-cell deprived mice with patent infections were not significantly different from those in uninfected ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182002002858

    authors: Stanley RG,Ngaiza JR,Atieno E,Jell G,Francklow K,Jackson CL,Parry H,Doenhoff MJ

    更新日期:2003-03-01 00:00:00

  • Host immune status affects maturation time in two nematode species--but not as predicted by a simple life-history model.

    abstract::In theory, the age at which maturation occurs in parasitic nematodes is inversely related to pre-maturational mortality rate, and cross-species data on mammalian nematodes are consistent with this prediction. Immunity is a major source of parasite mortality and parasites stand to gain sizeable fitness benefits through...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182003003998

    authors: Guinnee MA,Gemmill AW,Chan BH,Viney ME,Read AF

    更新日期:2003-11-01 00:00:00

  • Effect of the expulsion phase of Trichinella spiralis on Hymenolepis diminuta infection in mice.

    abstract::The rapid elimination of the intestinal phase of Trichinella spiralis in NIH mice is associated with progressive inflammation of the intestinal tract. The non-specific effects of this inflammation were studied in mice concurrently infected with an unrelated parasite, Hymenolepis diminuta, which does not stimulate a vi...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000048344

    authors: Behnke JM,Bland PW,Wakelin D

    更新日期:1977-08-01 00:00:00

  • The mannitol cycle in Eimeria.

    abstract::A metabolic pathway known as the mannitol cycle has been identified in Eimerian parasites. The pathway is a shunt off of the glycolytic pathway at fructose-6-phosphate (F6P). Two enzymes convert F6P to mannitol and two other enzymes are responsible for converting mannitol back to F6P when it is utilized. Although the ...

    journal_title:Parasitology

    pub_type: 杂志文章,评审

    doi:

    authors: Schmatz DM

    更新日期:1997-01-01 00:00:00

  • Mitochondria of mammalian Plasmodium spp.

    abstract::Highly purified mitochondrial fractions have been isolated from the intraerythrocytic stages of two mammalian Plasmodium spp., Plasmodium yoelii of rodents and Plasmodium falciparum of man. Mitochondria of the former parasite are cristate whereas those of the latter are essentially acristate. Isolated mitochondria fro...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000060297

    authors: Fry M,Beesley JE

    更新日期:1991-02-01 00:00:00

  • The interaction between the gelatin-binding domain of fibronectin and the attachment of Pasteuria penetrans endospores to nematode cuticle.

    abstract::Pasteuria penetrans is a Gram-positive endospore-producing bacterium that is a parasite of root-knot nematodes. Attachment of endospores to the cuticle of the nematode is the first stage in the infection process. Western blot analysis with monoclonal and polyclonal antibodies that recognize the 30 kDa heparin-binding ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182001008411

    authors: Mohan S,Fould S,Davies KG

    更新日期:2001-09-01 00:00:00

  • Sialic acid analysis and tritium-labelling of sialoglycoproteins of mouse erythrocytes infected with Plasmodium berghei.

    abstract::Schizont-infected red blood cells (SI-RBC) from Plasmodium berghei-infected mice contain between 2 and 10 times as much sialic acid as uninfected RBC from the same blood (99-550 micrograms/10(10) RBC versus 33-65 micrograms/10(10) RBC). Total RBC samples from infected animals containing up to 63% ring- and trophozoite...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000065434

    authors: Howard RJ,Seeley DC Jr,Kao V,Wember M,Schauer R

    更新日期:1986-06-01 00:00:00

  • Low levels of transforming growth factor-beta (TGF-beta) and reduced suppression of Th2-mediated inflammation in hyperreactive human onchocerciasis.

    abstract::Th2-biased inflammation with eosinophilia and IgE production is a hallmark of helminth infections. It is pronounced in hyperreactive onchocerciasis patients ('sowda' or 'local form'), who efficiently kill microfilariae resulting in severe dermatitis and lymphadenitis. In contrast, hyporeactive patients ('generalised f...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182010000922

    authors: Korten S,Hoerauf A,Kaifi JT,Büttner DW

    更新日期:2011-01-01 00:00:00

  • The effects of age, temperature, light quantity and wavelength on the swimming behaviour of the cercariae of Cryptocotyle lingua (Digenea: Heterophyidae).

    abstract::Laboratory experiments indicated that the active life-span of the cercariae of Cryptocotyle lingua was temperature-dependent. An increase in water temperature and population age both correlated with a tendency for the organisms to become decaudate. The larvae were strongly photoresponsive to lateral light but with tim...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000073789

    authors: Rea JG,Irwin SW

    更新日期:1992-08-01 00:00:00

  • The number and size of ovarian balls of Moniliformis (Acanthocephala) from laboratory rats.

    abstract::By means of conventional light microscopical and counting procedures, estimations have been made of the average number and size of ovarian balls from unfertilized and fertilized female Moniliformis dubius. The observations were made on worms recovered from experimental primary infections lasting 1, 2, 3, 4, 5, 8, 11, ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000051337

    authors: Crompton DW,Arnold S

    更新日期:1976-08-01 00:00:00

  • Schistosoma mansoni: changes in elemental composition in relation to the age and sexual status of the worms.

    abstract::Changes during development in the elemental contents (Ca, P, K, Mg and S) of male and female Schistosoma mansoni from both mixed and single sex infections have been measured for the first time. In mixed infections, there is a significant difference between the calcium content of male and female worms; female worms hav...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000050630

    authors: Shaw MK,Erasmus DA

    更新日期:1983-06-01 00:00:00

  • Characterization and immunolocalization of a main proteinaceous component of the cell wall of the protozoan parasite Perkinsus atlanticus.

    abstract::Described in the present study is a major component of the cell wall of 2 of the most pathogenic parasites of molluscs, Perkinsus atlanticus and P. marinus. The component is a high molecular weight protein (233 kDa), which we have named PWP-1 (for Perkinsus wall protein-1). Western blots, using a polyclonal serum gene...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182002001415

    authors: Montes JF,Durfort M,Lladó A,García-Valero J

    更新日期:2002-05-01 00:00:00

  • A phylogenetic analysis of filarial nematodes: comparison with the phylogeny of Wolbachia endosymbionts.

    abstract::Infection with the endosymbiotic bacteria Wolbachia is widespread in filarial nematodes. Previous studies have suggested concordance between the phylogeny of Wolbachia with that of their nematode hosts. However, there is only one published molecular phylogenetic study of filarial species, based on the 5S rRNA gene spa...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000007149

    authors: Casiraghi M,Anderson TJ,Bandi C,Bazzocchi C,Genchi C

    更新日期:2001-01-01 00:00:00

  • Differentiation of Schistosoma mansoni from S. rodhaini using cloned DNA probes.

    abstract::The ribosomal RNA (rRNA) gene units of Schistosoma mansoni and S. rodhaini, of the lateral spined egg group, have been studied. The schistosome rRNA gene unit consists of a regular interspersion of the two genes encoding the large and small rRNA units with two spacers. The large spacer is not transcribed while the sma...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000059709

    authors: Walker TK,Simpson AJ,Rollinson D

    更新日期:1989-02-01 00:00:00

  • Tight binding between a pool of the heterodimeric alpha/beta tubulin and a protein kinase CK2 in Trypanosoma cruzi epimastigotes.

    abstract::Tubulin is the predominant phosphoprotein in Trypanosoma cruzi epimastigotes and is phosphorylated by a protein kinase CK2. Interestingly, the presence or absence of divalent cations affected the solubilization of a pool of the parasite tubulin and the CK2 responsible for its phosphorylation. This fraction of tubulin ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182005009352

    authors: De Lima AR,Medina R,Uzcanga GL,Noris Suárez K,Contreras VT,Navarro MC,Arteaga R,Bubis J

    更新日期:2006-04-01 00:00:00

  • Dipstick dot ELISA for the detection of Taenia coproantigens in humans.

    abstract::A dipstick dot ELISA for detection of Taenia-specific coproantigens was developed. The test was based on a sandwich ELISA using antibodies raised against adult Taenia solium. Antibodies were absorbed to nitrocellulose paper previously adhered to acetate plastic to form dipsticks. Once blocked with 5% skimmed milk and ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000079439

    authors: Allan JC,Mencos F,Garcia-Noval J,Sarti E,Flisser A,Wang Y,Liu D,Craig PS

    更新日期:1993-07-01 00:00:00

  • Factors affecting surface changes in intact cercariae and cercarial bodies of Schistosoma mansoni.

    abstract::The effect of different incubation media and of temperature on the induction of water sensitivity has been investigated in intact and tailless Schistosoma mansoni cercariae. Removal of the cercarial tail by vortex stirring and elevation of the temperature of the medium from 27 to 37 degrees C resulted in the rapid ons...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000053105

    authors: Ramalho-Pinto FJ,Gazzinelli G,Howells RE,Pellegrino J

    更新日期:1975-08-01 00:00:00

  • Tick immunobiology.

    abstract::Ticks are of vast medical and veterinary public health importance due to direct damage caused by feeding and their roles in transmitting well known and emerging infectious agents. Ticks and tick-borne pathogens stimulate the immune system of the host. Those immune interactions are of importance in tick biology, pathog...

    journal_title:Parasitology

    pub_type: 杂志文章,评审

    doi:10.1017/s0031182004004834

    authors: Brossard M,Wikel SK

    更新日期:2004-01-01 00:00:00

  • Variation of transient gene expression within single lineages of Trypanosoma cruzi.

    abstract::The biodiversity of Trypanosoma cruzi is one of the main factors complicating the understanding of its molecular epidemiology. As an alternative to classical genetic methods, investigators have used DNA-mediated transformation techniques to study this diversity. Recently, transient expression data were shown to correl...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182099005053

    authors: Laurent JP,Swindle J

    更新日期:1999-12-01 00:00:00

  • Eimeria tenella in chickens: development of resistance to quinolone anticoccidial drugs.

    abstract::The development of drug resistance by the present Houghton strain of Eimeria tenella to the quinolones, methyl benzoquate and buquinolate, was found to take place after a single experimental passage. The development of resistance was independent of drug selection pressure and showed cross resistance to other quinolone...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000053130

    authors: Chapman HD

    更新日期:1975-08-01 00:00:00

  • Identification and diagnosis of Leishmania mexicana complex isolates by polymerase chain reaction.

    abstract::Following cloning of Leishmania (L.) amazonensis kinetoplast DNA two recombinant clones were identified: one specific for L. (L.) amazonensis and the other specific for L. (L.) amazonensis and closely related isolates. DNA sequences from these clones were compared with those of other kinetoplastids and oligonucleotide...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000080677

    authors: Eresh S,McCallum SM,Barker DC

    更新日期:1994-11-01 00:00:00

  • Neoglycosylated liposomes as efficient ligands for the evaluation of specific sugar receptors on macrophages in health and in experimental leishmaniasis.

    abstract::Receptors interacting with terminal sugars as ligands are involved in the binding of Leishmania donovani promastigotes to the macrophage surface and their subsequent internalization. Mannose and glucose are specifically involved in the binding process. Decreased binding occurs to macrophages already infected with L. d...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000076253

    authors: Dutta M,Bandyopadhyay R,Basu MK

    更新日期:1994-08-01 00:00:00

  • Enzymatic amplification of mini-exon-derived RNA gene spacers of Leishmania donovani: primers and probes for DNA diagnosis.

    abstract::The multicopy mini-exon-derived RNA (med RNA) locus of Leishmania donovani was enzymatically amplified by the polymerase chain reaction (PCR). The major 180 bp PCR product contained conserved med RNA gene sequences flanking the variable intergenic spacer from the med RNA gene tandem repeat. The oligonucleotide primers...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000068086

    authors: Hassan MQ,Ghosh A,Ghosh SS,Gupta M,Basu D,Mallik KK,Adhya S

    更新日期:1993-12-01 00:00:00

  • Label-free enrichment of avian Leucocytozoon using flow cytometric sorting.

    abstract::The group of haemosporidian parasites is of general interest to basic and applied science, since several species infect mammals, leading to malaria and associated disease symptoms. Although the great majority of haemosporidian parasites appear in bird hosts, as in the case of Leucocytozoon buteonis, there is little ge...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182012000972

    authors: Chakarov N,Greiner JF,Hauser S,Schuetzmann D,Krüger O,Boerner M,Wıdera D

    更新日期:2012-10-01 00:00:00

  • Towards a comprehensive simulation model of malaria epidemiology and control.

    abstract::Planning of the control of Plasmodium falciparum malaria leads to a need for models of malaria epidemiology that provide realistic quantitative prediction of likely epidemiological outcomes of a wide range of control strategies. Predictions of the effects of control often ignore medium- and long-term dynamics. The com...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182008000371

    authors: Smith T,Maire N,Ross A,Penny M,Chitnis N,Schapira A,Studer A,Genton B,Lengeler C,Tediosi F,de Savigny D,Tanner M

    更新日期:2008-11-01 00:00:00