Immunolocalization and characterization of the low molecular weight antigen (4-5 kDa) of Toxoplasma gondii that elicits an early IgM response upon primary infection.

Abstract:

:A striking feature of toxoplasmic seroconversion is the prominent and early IgM response to a low molecular weight antigen of 4-5 kDa. Two different monoclonal antibodies directed against the 4-5 kDa antigen have been generated and used to characterize this molecule. Using these monoclonal antibodies, we could demonstrate the surface localization of the low M(r) antigen by immunofluorescence and immuno-electron microscopy assays. By immunoblotting, we observed that one of the monoclonal antibodies was unable to recognize the 4-5 kDa antigen in tachyzoites propagated in cell culture, indicating an epitope variability between Toxoplasma gondii tachyzoites grown in vivo and in vitro. We discuss the implications of this latter finding in the design of diagnostic reagents.

journal_name

Parasitology

journal_title

Parasitology

authors

Tomavo S,Couvreur G,Leriche MA,Sadak A,Achbarou A,Fortier B,Dubremetz JF

doi

10.1017/s0031182000068220

subject

Has Abstract

pub_date

1994-02-01 00:00:00

pages

139-45

eissn

0031-1820

issn

1469-8161

journal_volume

108 ( Pt 2)

pub_type

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