Abstract:
:A striking feature of toxoplasmic seroconversion is the prominent and early IgM response to a low molecular weight antigen of 4-5 kDa. Two different monoclonal antibodies directed against the 4-5 kDa antigen have been generated and used to characterize this molecule. Using these monoclonal antibodies, we could demonstrate the surface localization of the low M(r) antigen by immunofluorescence and immuno-electron microscopy assays. By immunoblotting, we observed that one of the monoclonal antibodies was unable to recognize the 4-5 kDa antigen in tachyzoites propagated in cell culture, indicating an epitope variability between Toxoplasma gondii tachyzoites grown in vivo and in vitro. We discuss the implications of this latter finding in the design of diagnostic reagents.
journal_name
Parasitologyjournal_title
Parasitologyauthors
Tomavo S,Couvreur G,Leriche MA,Sadak A,Achbarou A,Fortier B,Dubremetz JFdoi
10.1017/s0031182000068220subject
Has Abstractpub_date
1994-02-01 00:00:00pages
139-45eissn
0031-1820issn
1469-8161journal_volume
108 ( Pt 2)pub_type
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