Abstract:
:Most of our knowledge about the process of penetration of skin, by cercariae of Schistosoma mansoni, has been gained from studies carried out in vivo with laboratory animals. Human skin is significantly different from that of other animals but there are obvious practical difficulties in directly studying attachment and penetration with human skin. Techniques have been developed which enable a 3-dimensional skin equivalent to be grown in tissue culture, made from different types of human skin cells. The aim of the present study was to investigate cercarial interactions with confluent cultures of the individual skin cell types that make up normal human skin and which will be used to construct a multi-component model. Cercariae behaved differently towards the various cell types tested. They responded least to monolayers of endothelial cells and most to primary keratinocytes, derived from human foreskin and differentiated at an air/liquid interface. This study demonstrates, therefore, that cercariae are capable of distinguishing between different types of skin cells and they preferentially attach to differentiated cells which form the epidermis.
journal_name
Parasitologyjournal_title
Parasitologyauthors
Khammo N,Bartlett A,Clothier RH,Whitfield PJdoi
10.1017/s003118200100885xsubject
Has Abstractpub_date
2002-01-01 00:00:00pages
25-30issue
Pt 1eissn
0031-1820issn
1469-8161journal_volume
124pub_type
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