Substrate specificity of the neutral sphingomyelinase from Trypanosoma brucei.

Abstract:

:The kinetoplastid parasite Trypanosoma brucei causes African trypanosomiasis in both humans and animals. Infections place a significant health and economic burden on developing nations in sub-Saharan Africa, but few effective anti-parasitic treatments are currently available. Hence, there is an urgent need to identify new leads for drug development. The T. brucei neutral sphingomyelinase (TbnSMase) was previously established as essential to parasite survival, consequently being identified as a potential drug target. This enzyme may catalyse the single route to sphingolipid catabolism outside the T. brucei lysosome. To obtain new insight into parasite sphingolipid catabolism, the substrate specificity of TbnSMase was investigated using electrospray ionization tandem mass spectrometry (ESI-MS/MS). Recombinant TbnSMase was shown to degrade sphingomyelin, inositol-phosphoceramide and ethanolamine-phosphoceramide sphingolipid substrates, consistent with the sphingolipid complement of the parasites. TbnSMase also catabolized ceramide-1-phosphate, but was inactive towards sphingosine-1-phosphate. The broad-range specificity of this enzyme towards sphingolipid species is a unique feature of TbnSMase. Additionally, ESI-MS/MS analysis revealed previously uncharacterized activity towards lyso-phosphatidylcholine despite the enzyme's inability to degrade phosphatidylcholine. Collectively, these data underline the enzyme's importance in choline homoeostasis and the turnover of sphingolipids in T. brucei.

journal_name

Parasitology

journal_title

Parasitology

authors

Dickie EA,Young SA,Smith TK

doi

10.1017/S0031182018001853

subject

Has Abstract

pub_date

2019-04-01 00:00:00

pages

604-616

issue

5

eissn

0031-1820

issn

1469-8161

pii

S0031182018001853

journal_volume

146

pub_type

杂志文章
  • Isolation and genetic characterization of Neospora caninum from asymptomatic calves in Spain.

    abstract::Neospora caninum is a cyst-forming parasite that causes abortion in cattle. Despite this parasite's ubiquitous distribution and wide host range, the number of N. caninum isolates obtained to date is limited. In vitro isolation of the parasite is arduous and often unsuccessful. In addition, most isolates have been obta...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S003118200800509X

    authors: Regidor-Cerrillo J,Gómez-Bautista M,Pereira-Bueno J,Aduriz G,Navarro-Lozano V,Risco-Castillo V,Férnandez-García A,Pedraza-Díaz S,Ortega-Mora LM

    更新日期:2008-12-01 00:00:00

  • Criteria for a proof of migration routes of immature parasites inside hosts exemplified by studies of Strongyloides ratti in the rat.

    abstract::The first rigorous proof applicable to the migration pathway of an infective juvenile macroparasite inside its host is presented. Third-stage larvae of a homogonic strain of Strongyloides ratti applied in exact doses of less than 20 to the skin of the flank of young rats were recovered 16-40 h later in the naso-fronta...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000080185

    authors: Tindall NR,Wilson PA

    更新日期:1988-06-01 00:00:00

  • Features of amino acid metabolism in Moniliformis moniliformis (Acanthocephala) in vitro.

    abstract::Experiments to investigate the metabolism of glycine, L-glutamic acid and L-aspartic acid by Moniliformis moniliformis were carried out by incubating adult worms aerobically for 3 h at 37 degrees C in Tyrode's solution containing either [U-14C]glycine, L-[U-14C]glutamic acid, L-[U-14C]aspartic acid or L-[4-14C]asparti...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000055876

    authors: Ward PF,Crompton DW

    更新日期:1987-06-01 00:00:00

  • The number and size of ovarian balls of Moniliformis (Acanthocephala) from laboratory rats.

    abstract::By means of conventional light microscopical and counting procedures, estimations have been made of the average number and size of ovarian balls from unfertilized and fertilized female Moniliformis dubius. The observations were made on worms recovered from experimental primary infections lasting 1, 2, 3, 4, 5, 8, 11, ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000051337

    authors: Crompton DW,Arnold S

    更新日期:1976-08-01 00:00:00

  • Label-free enrichment of avian Leucocytozoon using flow cytometric sorting.

    abstract::The group of haemosporidian parasites is of general interest to basic and applied science, since several species infect mammals, leading to malaria and associated disease symptoms. Although the great majority of haemosporidian parasites appear in bird hosts, as in the case of Leucocytozoon buteonis, there is little ge...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182012000972

    authors: Chakarov N,Greiner JF,Hauser S,Schuetzmann D,Krüger O,Boerner M,Wıdera D

    更新日期:2012-10-01 00:00:00

  • Widespread infection of the Eastern red-spotted newt (Notophthalmus viridescens) by a new species of Amphibiocystidium, a genus of fungus-like mesomycetozoan parasites not previously reported in North America.

    abstract::Given the worldwide decline of amphibian populations due to emerging infectious diseases, it is imperative that we identify and address the causative agents. Many of the pathogens recently implicated in amphibian mortality and morbidity have been fungal or members of a poorly understood group of fungus-like protists, ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182007003708

    authors: Raffel TR,Bommarito T,Barry DS,Witiak SM,Shackelton LA

    更新日期:2008-02-01 00:00:00

  • Observations on the surface morphology of the ovarian balls of Moniliformis (Acanthocephala).

    abstract::The surface morphology of fixed ovarian balls from female Moniliformis dubius, agd 12, 18, 26, 28, 42, 77, 105 and 118 days, has been examined by means of scanning electron microscopy. The appearance of the surface has been found to be variable between ovarian balls from worms of the same and different ages. Some part...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000056146

    authors: Parshad VR,Crompton DW,Martin J

    更新日期:1980-10-01 00:00:00

  • Fasciola hepatica: the ultrastructure of the epithelium of the seminal vesicle, the ejaculatory duct and the cirrus.

    abstract::The ultrastructure of the seminal vesicle, ejaculatory duct, cirrus sac and cirrus is described. The epithelium of the seminal vesicle consists of a single layer of squamous to cuboidal cells. The apical ends of the cells have thin polymorphic lamellae and long narrow pits, both of which enclose normal spermatozoa. Th...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s003118200004720x

    authors: Threadgold LT

    更新日期:1975-12-01 00:00:00

  • Host-parasite relationship between congenital Toxoplasma infection and mouse brain: role of small vessels.

    abstract::Small vessels showing inflammatory cell infiltrates were invariably observed in the vicinity of intact Toxoplasma tissue cysts within the brains of mice congenitally infected with the protozoan. Lymphocytes were observed in intimate contact with the luminal aspect of the endothelium, penetrating into the thickened bas...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000063873

    authors: Sims TA,Hay J

    更新日期:1995-02-01 00:00:00

  • Association and predatory capacity of fungi Pochonia chlamydosporia and Arthrobotrys cladodes in the biological control of parasitic helminths of bovines.

    abstract::Nematophagous fungi are used in the biological control of the parasitic helminths of animals and plants. The association of ovicidal and predator nematophagous fungi may present a complementary and increased action on the biological control of helminths. Joint growth compatibility and predation tests were carried out ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S003118201900060X

    authors: Vieira ÍS,Oliveira IC,Campos AK,Araújo JV

    更新日期:2019-09-01 00:00:00

  • Eimeria tenella in chickens: development of resistance to quinolone anticoccidial drugs.

    abstract::The development of drug resistance by the present Houghton strain of Eimeria tenella to the quinolones, methyl benzoquate and buquinolate, was found to take place after a single experimental passage. The development of resistance was independent of drug selection pressure and showed cross resistance to other quinolone...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000053130

    authors: Chapman HD

    更新日期:1975-08-01 00:00:00

  • Phylogeny of Trypanosoma ( Megatrypanum ) theileri and related trypanosomes reveals lineages of isolates associated with artiodactyl hosts diverging on SSU and ITS ribosomal sequences.

    abstract::SSU ribosomal sequences of trypanosomes from Brazilian cattle and water buffalo were used to infer phylogenetic relationships between non-pathogenic T. theileri and allied species parasitic in artiodactyls. T. theileri trypanosomes from distinct geographical regions in Brazil and from other countries were tightly clus...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182005008929

    authors: Rodrigues AC,Paiva F,Campaner M,Stevens JR,Noyes HA,Teixeira MM

    更新日期:2006-02-01 00:00:00

  • Changes in the somatic musculature of the 4th-stage larvae of Ascaris suum Goeze, 1782 (Nematoda: Ascaridoidea) developing in swine.

    abstract::Fourth-stage larvae of Ascaris suum, recovered from piglets at 11-22 days post-infection (p.i.), were examined by light microscopy for changes in the somatic musculature. During this time the estimated total number of muscle cells in the body increases from approximately 600 to 21 000 cells. This non-eutelic developme...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s003118200005441x

    authors: O'Grady RT

    更新日期:1984-02-01 00:00:00

  • Genetics: modes of reproduction and genetic analysis.

    abstract::Classical and reverse genetics remain invaluable tools for the scientific investigation of model organisms. Genetic analysis of endoparasites is generally difficult because the sexual adults required for crossing and other manipulations are usually hidden within their host. Strongyloides spp. and Parastrongyloides spp...

    journal_title:Parasitology

    pub_type: 杂志文章,评审

    doi:10.1017/S0031182016000342

    authors: Streit A

    更新日期:2017-03-01 00:00:00

  • Morphological and molecular characterization of Plasmodium cathemerium (lineage PADOM02) from the sparrow Passer domesticus with complete sporogony in Culex pipiens complex.

    abstract::Avian malaria is a mosquito-borne disease caused by Plasmodium spp. protozoa. Although these parasites have been extensively studied in North America and Eurasia, knowledge on the diversity of Plasmodium, its vectors and avian hosts in Africa is scarce. In this study, we report on natural malarial infections in free-r...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182020000566

    authors: Aly MZY,Mohamed III,Sebak SI,Vanstreels RET,El Gendy AM

    更新日期:2020-08-01 00:00:00

  • Isolation and characterization of an avian isolate of Encephalitozoon hellem.

    abstract::Members of the phylum Microspora are a group of unusual, obligate intracellular eukaryotic parasites that infect a wide range of hosts. However, there are a limited number of microsporidial infections reported in avian hosts, and no parasite species has been defined as an avian pathogen. A microsporidian organism was ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182099005995

    authors: Snowden KF,Logan K,Phalen DN

    更新日期:2000-07-01 00:00:00

  • Soil-transmitted helminth infections in school children from Cocle Province, Republic of Panama.

    abstract::A survey was carried out to determine values for the prevalence and intensity of infection of intestinal helminths in children living in Cocle Province, Republic of Panama. The data set consisted of the results of microscopic examinations (modified Kato Katz technique) of stool samples collected in November 1987 from ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000058741

    authors: Robertson LJ,Crompton DW,Walters DE,Nesheim MC,Sanjur D,Walsh EA

    更新日期:1989-10-01 00:00:00

  • Understanding transmissibility patterns of Chagas disease through complex vector-host networks.

    abstract::Chagas disease is one of the most important vector-borne zoonotic diseases in Latin America. Control strategies could be improved if transmissibility patterns of its aetiologic agent, Trypanosoma cruzi, were better understood. To understand transmissibility patterns of Chagas disease in Mexico, we inferred potential v...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182016002468

    authors: Rengifo-Correa L,Stephens CR,Morrone JJ,Téllez-Rendón JL,González-Salazar C

    更新日期:2017-05-01 00:00:00

  • Comparison of cysteine peptidase activities in Trichobilharzia regenti and Schistosoma mansoni cercariae.

    abstract::Cercariae of the bird schistosome Trichobilharzia regenti and of the human schistosome Schistosoma mansoni employ proteases to invade the skin of their definitive hosts. To investigate whether a similar proteolytic mechanism is used by both species, cercarial extracts of T. regenti and S. mansoni were biochemically ch...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182007002910

    authors: Kasný M,Mikes L,Dalton JP,Mountford AP,Horák P

    更新日期:2007-10-01 00:00:00

  • Membrane localization and demonstration of isoforms of nucleoside triphosphate hydrolase from Toxoplasma gondii.

    abstract::Toxoplasma gondii has a unique enzyme, a NTPase, which has a wide specificity toward NTP. In the present study, we produced a monoclonal antibody (IgG1, 6C6) against the enzyme which could recognize NTPase isozymes among several strains of T. gondii. Three avirulent strains of T. gondii, ME49, Beverley and Nakayama, w...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000007101

    authors: Kikuchi T,Furuta T,Kojima S

    更新日期:2001-01-01 00:00:00

  • A Cooperia punctata gene family encoding 14 kDa excretory-secretory antigens conserved for trichostrongyloid nematodes.

    abstract::A polymorphic set of 14 kDa excretory-secretory (E-S) antigen-encoding cDNAs, with similarity to a previously characterized 15 kDa E-S antigen of Haemonchus contortus, was cloned from Cooperia punctata. Five cDNAs encoding predicted proteins of 70-80% identity were sequenced. Genomic analyses of individuals proved the...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182001008770

    authors: Yatsuda AP,De Vries E,Vieira Bressan MC,Eysker M

    更新日期:2001-12-01 00:00:00

  • Phylogeny of diplozoids in five genera of the subfamily Diplozoinae Palombi, 1949 as inferred from ITS-2 rDNA sequences.

    abstract::The phylogenetic relationship of 5 genera, i.e. Diplozoon Nordmann, 1832, Paradiplozoon Achmerov, 1974, Inustiatus Khotenovsky, 1978, Sindiplozoon Khotenovsky, 1981, and Eudiplozoon Khotenovsky, 1985 in the subfamily Diplozoinae Palombi, 1949 (Monogenea, Polyopisthocotylea) was inferred from rDNA ITS-2 region using ne...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182006001971

    authors: Gao Q,X Chen M,Yao WJ,Gao Y,Song Y,Wang GT,Wang MX,Nie P

    更新日期:2007-05-01 00:00:00

  • Evidence that primary infection of Charollais sheep with Toxoplasma gondii may not prevent foetal infection and abortion in subsequent lambings.

    abstract::A study carried out on a sheep farm examined whether Toxoplasma gondii foetal infection and associated abortion occur in successive lambings. We identified 29 ewes that gave birth to lambs on at least 2 successive years over our study period, 2000-2003. Tissue samples from the progeny of these ewes were analysed by PC...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182007003721

    authors: Morley EK,Williams RH,Hughes JM,Thomasson D,Terry RS,Duncanson P,Smith JE,Hide G

    更新日期:2008-02-01 00:00:00

  • Molecular phylogenetic and morphological analysis of haemosporidian parasites (Haemosporida) in a naturally infected European songbird, the blackcap Sylvia atricapilla, with description of Haemoproteus pallidulus sp. nov.

    abstract::The blackcap (Sylvia atricapilla) is a common Palearctic migratory warbler, and haemosporidian parasites are common in this species. However, genetic and phenotypic diversity of haemosporidians in warblers has been insufficiently investigated and poorly linked. We addressed this issue by combining molecular and micros...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182009991235

    authors: Krizanauskiene A,Pérez-Tris J,Palinauskas V,Hellgren O,Bensch S,Valkiūnas G

    更新日期:2010-02-01 00:00:00

  • New defined and semi-defined media for cultivation of the flagellate Trichomonas vaginalis.

    abstract::Studies on the nutrition and physiology of Trichomonas vaginalis have been hampered by the lack of a defined synthetic medium in which to grow the organism. New synthetic media free of ill-defined supplements and serum which support good growth of T. vaginalis have been developed in this study. The first successful ap...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000050101

    authors: Linstead D

    更新日期:1981-08-01 00:00:00

  • Hatching in the monogenean parasite Dictyocotyle coeliaca from the body cavity of Raja naevus.

    abstract::When eggs of the monogenean Dictyocotyle coeliaca, a parasite from the body cavity of Raja naevus, are incubated in alternating 12 h periods of light and darkness at 12 degrees C the marginal hooklets appear between 75 and 90 days after collecting the eggs from the host's body cavity and hatching begins after about 10...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000048897

    authors: Kearn GC

    更新日期:1975-02-01 00:00:00

  • Maltose utilization by extracellular hydrolysis followed by glucose transport in Trichomonas vaginalis.

    abstract::The amitochondriate parasitic protist Trichomonas vaginalis can utilize either glucose or maltose as carbon and energy source. The mechanisms of maltose utilization were explored with uptake experiments using radio-isotope labelled maltose in combination with the silicone-oil centrifugation technique and enzymatic ass...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000081026

    authors: ter Kuile BH,Müller M

    更新日期:1995-01-01 00:00:00

  • Eimeria tenella, E. acervulina and E. maxima: studies on the development of resistance to diclazuril and other anticoccidial drugs in the chicken.

    abstract::Resistance to diclazuril was induced by 10 passages of the Houghton strains of Eimeria acervulina and E. tenella in chickens given progressively greater concentrations of the drug. This resistance was, however, not complete since the drug retained some efficacy against the drug-passaged lines. Attempts to passage the ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000058625

    authors: Chapman HD

    更新日期:1989-10-01 00:00:00

  • Cystic echinococcosis: analysis of the serological profile related to the risk factors in individuals without ultrasound liver changes living in an endemic area of Tacuarembó, Uruguay.

    abstract::Cystic echinococcosis (CE) is a high prevalence zoonosis among the rural population of Tacuarembó (Uruguay). The correlation between serological data and the incidence of risk factors was studied in a survey carried out in 1998 among rural communities where 480 individuals were examined by means of abdominal sonograph...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182004006717

    authors: Hernández A,Cardozo G,Dematteis S,Baz A,Trias N,Nuñez H,Barragué A,López L,Fuentes J,López O,Ferreira C

    更新日期:2005-04-01 00:00:00

  • Interaction of the proteasome S5a/Rpn10 multiubiquitin-binding protein and the 8 kDa calcium-binding protein of Schistosoma mansoni.

    abstract::A distinct 8 kDa calcium-binding protein (CaBP) is preferentially expressed at the cercarial stage during the life-cycle of the schistosome. Available data indicate that this CaBP may be associated with tissue/organ remodelling (involving protein degradation and synthesis of new proteins) during transformation of the ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182003003858

    authors: Ram D,Ziv E,Lantner F,Schechter I

    更新日期:2003-10-01 00:00:00