Abstract:
:Promastigotes of Leishmania braziliensis panamensis absorbed the purines adenine, hypoxanthine, adenosine and inosine by a combination of diffusion and mediated components. When the uptake rates for these substrates were corrected for diffusion and compared, the purine bases adenine and hypoxanthine were transported at a significantly slower rate than the purine nucleosides adenosine and inosine. Competitive interactions among those purines tested confirmed the presence of mediated and diffusion components and suggested that three transport loci may be operating (Fig. 6). The first transport locus, designated Locus 1, transported inosine, Locus 2, the purine bases hypoxanthine and adenine and Locus 3, adenosine. In addition, adenine and hypoxanthine inhibited the uptake of one another competitively. A comparison of Ki values derived from double reciprocal plots of labelled hypoxanthine and adenine uptake in the presence of the unlabelled substrates as inhibitors suggested that adenine has a greater affinity for the transport locus.
journal_name
Parasitologyjournal_title
Parasitologyauthors
Hansen BD,Perez-Arbelo J,Walkony JF,Hendricks LDdoi
10.1017/s0031182000055256subject
Has Abstractpub_date
1982-10-01 00:00:00pages
271-82eissn
0031-1820issn
1469-8161journal_volume
85 (Pt 2)pub_type
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