The specificity of purine base and nucleoside uptake in promastigotes of Leishmania braziliensis panamensis.

Abstract:

:Promastigotes of Leishmania braziliensis panamensis absorbed the purines adenine, hypoxanthine, adenosine and inosine by a combination of diffusion and mediated components. When the uptake rates for these substrates were corrected for diffusion and compared, the purine bases adenine and hypoxanthine were transported at a significantly slower rate than the purine nucleosides adenosine and inosine. Competitive interactions among those purines tested confirmed the presence of mediated and diffusion components and suggested that three transport loci may be operating (Fig. 6). The first transport locus, designated Locus 1, transported inosine, Locus 2, the purine bases hypoxanthine and adenine and Locus 3, adenosine. In addition, adenine and hypoxanthine inhibited the uptake of one another competitively. A comparison of Ki values derived from double reciprocal plots of labelled hypoxanthine and adenine uptake in the presence of the unlabelled substrates as inhibitors suggested that adenine has a greater affinity for the transport locus.

journal_name

Parasitology

journal_title

Parasitology

authors

Hansen BD,Perez-Arbelo J,Walkony JF,Hendricks LD

doi

10.1017/s0031182000055256

subject

Has Abstract

pub_date

1982-10-01 00:00:00

pages

271-82

eissn

0031-1820

issn

1469-8161

journal_volume

85 (Pt 2)

pub_type

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