Abstract:
:The host recognition and invasion process of Myxobolus cerebralis actinospores (triactinomyxon, TAM) was studied on a genetic level. A small-scale in vitro assay was developed to activate a large number of TAMs simultaneously, and to monitor the host invasion in the absence of live fish. The transcriptomes of non-activated and in vitro-activated TAMs were compared by suppressive subtractive hybridization (SSH) to identify parasite genes involved in the host invasion process. Differential screening and a subsequent BLAST search revealed 15 of 452 SSH-library clones expressed differently in activated TAMs. None of the 15 transcripts obtained has previously been identified from M. cerebralis. Quantitative real-time PCR was used to examine the relative expression profile of 8 selected transcripts upon TAM activation and after penetration of the host. Four of these were found to be up-regulated in activated TAMs, while expression was relatively low in non-activated TAMs and in infected fish tissue, indicating that they are relevant genes during host recognition or subsequent host invasion of M. cerebralis TAMs.
journal_name
Parasitologyjournal_title
Parasitologyauthors
Eszterbauer E,Kallert DM,Grabner D,El-Matbouli Mdoi
10.1017/S0031182008005398subject
Has Abstractpub_date
2009-04-01 00:00:00pages
367-77issue
4eissn
0031-1820issn
1469-8161pii
S0031182008005398journal_volume
136pub_type
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