Abstract:
:Intact, viable adults, infective and newborn larvae of Trichinella pseudospiralis were surface labelled with 125I by the chloramine T method and labelled proteins were compared with those obtained from equivalent stages of T. spiralis. Electron-microscope autoradiography determined that labelled proteins were restricted to the cuticle for all stages of both isolates. Comparative polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate (SDS-PAGE), using thin gradient gel slabs, of proteins obtained from each stage, demonstrated that the profile of surface-labelled proteins of T. pseudospiralis were restricted in number, stage specific, and similar to equivalent proteins of T. spiralis both in size and in their organization into aggregates. The stage-specific profiles of surface-labelled proteins derived from newborn larvae were indistinguishable, but differences were noted between adults and infective larvae of the two isolates. These differences in protein structure were confirmed by two dimensional mapping of tryptic peptides. Stage-specific profiles were also obtained when [35S]methionine biosynthetically labelled secretions of the 3 stages of T. pseudospiralis were compared by SDS-PAGE. Comparison of the profiles obtained with secretions for respective stages of T. spiralis again failed to distinguish newborn larvae, but adults and infective larvae of T. spiralis and T. pseudospiralis displayed a mixture of common and species-specific proteins. These findings are discussed in relation to the different pathology associated with infection with two isolates.
journal_name
Parasitologyjournal_title
Parasitologyauthors
Almond NM,McLaren DJ,Parkhouse RMdoi
10.1017/s003118200004991xsubject
Has Abstractpub_date
1986-08-01 00:00:00pages
163-76eissn
0031-1820issn
1469-8161journal_volume
93 ( Pt 1)pub_type
杂志文章相关文献
PARASITOLOGY文献大全abstract::Previous studies of mating interactions between schistosome species from different evolutionary lineages in mice showed that mating is not random: heterospecific pairs form but, given the choice, the preference is for homospecific partners. Mating competition and change of mate were also demonstrated. Schistosoma mans...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182002002111
更新日期:2002-09-01 00:00:00
abstract::The levels of superoxide dismutase (SOD) were determined in detergent-soluble, somatic and excretion-secretion (E-S) preparations from adult Fasciola hepatica using the xanthine oxidase system and visualized in substrate gels. Compared to detergent-soluble and somatic extracts, E-S products showed the highest SOD acti...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182098003394
更新日期:1998-12-01 00:00:00
abstract::This study quantifies the rate and intensity of re-infection with human hookworm and Schistosoma mansoni infection 12 months following successful treatment, and investigates the influence of socio-economic, geographical and environmental factors. A longitudinal study of 642 individuals aged over 5 years was conducted ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182011001132
更新日期:2011-09-01 00:00:00
abstract::Host behavioural modification by parasites is a common and well-documented phenomenon. However, knowledge on the complexity and specificity of the underlying mechanisms is limited, and host specificity among manipulating parasites has rarely been experimentally verified. We tested the hypothesis that the ability to in...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182015001171
更新日期:2015-11-01 00:00:00
abstract::The effects of the phenothiazine, Stelazine, on Hymenolepis diminuta were investigated. The cestode was incubated for 10 min at 37 degrees C with 1 mM trifluoperazine, in the presence and absence of Ca2+. Assay of brush border enzymes showed that drug treatment lowered the activities of alkaline phosphatase, Ca2+-ATP'...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s003118200005352x
更新日期:1987-02-01 00:00:00
abstract::A method has been devised to show that malaria parasites can be detected serologically in infected blood with a high degree of sensitivity. Using a murine malaria model, parasites were demonstrated in a solid-phase radio-immunoassay which measured antibody-binding inhibition. Lysed red blood cells (r.b.c.) were incuba...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000000639
更新日期:1980-02-01 00:00:00
abstract::Canine leishmaniosis (CanL) is caused by the parasite Leishmania infantum and is a systemic disease, which can present with variable clinical signs, and clinicopathological abnormalities. Clinical manifestations can range from subclinical infection to very severe systemic disease. Leishmaniosis is categorized as a neg...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S003118201600055X
更新日期:2017-01-01 00:00:00
abstract::Urinary schistosomiasis is an important source of human morbidity in Msambweni, Kenya, where the intermediate host snail, Bulinus nasutus is found in ponds and water pools. In the past, aquatic habitats in the area have been studied separately; however, recent collections of B. nasutus snails and shells indicated that...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182006001594
更新日期:2007-05-01 00:00:00
abstract::In a population of copepods (Cyclops strenuus abyssorum), with a naturally high prevalence of infection with procercoids of Diphyllobothrium spp., no difference in body size was found between infected and uninfected hosts. However, a significant reduction in the reproductive capacity of infected females was observed, ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000064738
更新日期:1995-05-01 00:00:00
abstract::The outcome of a parasite infection is the result of the interaction between the host and the parasite. In the system we studied, there are 3 critical stages for the outcome of infection of the (intermediate) host, the copepod Macrocyclops albidus, with the cestode Schistocephalus solidus. During the establishment pha...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182001001275
更新日期:2002-04-01 00:00:00
abstract::The efficiency of treatment of human infections with the unicellular eukaryotic pathogens such as fungi and protozoa remains deeply unsatisfactory. For example, the mortality rates from nosocomial fungemia in critically ill, immunosuppressed or post-cancer patients often exceed 50%. A set of six systemic clinical azol...
journal_title:Parasitology
pub_type: 杂志文章,评审
doi:10.1017/S0031182018000562
更新日期:2018-12-01 00:00:00
abstract::We investigated the segregation of an intracellular microsporidian parasite during host cell division. A time-course experiment was carried out to examine the distribution of parasites relative to host chromosomal DNA via light and electron microscopy. Fluorescent light microscopy and EM studies showed that the parasi...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182098003540
更新日期:1999-01-01 00:00:00
abstract::Neurocysticercosis (NCC) is the most common parasitic disease of the central nervous system (CNS) caused by the larval form of the tapeworm Taenia solium. NCC has a long asymptomatic period with little or no inflammation, and the sequential progression to symptomatic NCC depends upon the intense inflammation associate...
journal_title:Parasitology
pub_type: 杂志文章,评审
doi:10.1017/S0031182009991971
更新日期:2010-03-01 00:00:00
abstract::Epidemiological rate parameters of host generalist parasites are difficult to estimate, especially in cases where variation in parasite performance can be attributed to host species. Such cases are likely common for generalist parasites of sympatric grazing mammals. In this study, we combined data from experimental ex...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182015000621
更新日期:2015-09-01 00:00:00
abstract::We examined the effects of Haemoproteus infection on the survival and pathology caused in the biting midges. Forty-six females of Culicoides impunctatus were exposed experimentally by allowing them to feed on a naturally infected red-backed shrike infected with Haemoproteus lanii (lineage hRB1, gametocytaemia 5·2%). S...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182016001426
更新日期:2016-11-01 00:00:00
abstract::We conducted the first comprehensive study on the spatiotemporal structure of trematode communities in the large-mouthed valve snail, Valvata macrostoma. A total of 1103 snails were examined monthly between May and October 2007 from Lake Konnevesi, Central Finland, from a shallow (1-2 m deep) and an offshore site (5-6...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182008005027
更新日期:2008-12-01 00:00:00
abstract::Hymenolepis microstoma from secondary infections in mice were found to grow initially more slowly than H. microstoma from primary infections. Eventually a similar rate of growth was attained by both kinds of worm. After transplantation, young worms (less than or equal to 4 days old) grew more slowly in previously infe...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000062387
更新日期:1977-10-01 00:00:00
abstract::A dipstick dot ELISA for detection of Taenia-specific coproantigens was developed. The test was based on a sandwich ELISA using antibodies raised against adult Taenia solium. Antibodies were absorbed to nitrocellulose paper previously adhered to acetate plastic to form dipsticks. Once blocked with 5% skimmed milk and ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000079439
更新日期:1993-07-01 00:00:00
abstract::The biological parameters which generate seasonal fluctuations in the size of populations of Caryophyllaeus laticeps are examined in detail. A mathematical model is formulated to describe the dynamics of the adult parasite within the fish host Abramis brama, and the predictions of the model are compared with observed ...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000049490
更新日期:1976-06-01 00:00:00
abstract::The interactions in vivo between Plasmodium berghei sporozoites and Kupffer cells in rat livers were studied by transmission electron microscopy. By 10 and 15 min after inoculation, sporozoites were both free in the liver sinusoids and inside endocytotic vacuoles of the Kupffer cells. The latter cells were very active...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s003118200005040x
更新日期:1983-04-01 00:00:00
abstract::Sporulated oocysts or oocyst hulls of Eimeria acervulina given via the crop, and oocyst juice or sporozoites inoculated directly into the intestinal lumen, were tested for their effects on the hypersensitivity of the intestines of immunized and susceptible birds. Only viable sporozoites consistently caused an increase...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000047132
更新日期:1975-12-01 00:00:00
abstract::The SICA[-] or non-agglutinable phenotype of Plasmodium knowlesi schizont-infected erythrocytes has been defined serologically but not biochemically. Similarly, non-cloned SICA[+] or agglutinable parasites have been shown serologically to express SICA or variant antigen(s) but the number and nature of such antigens ha...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000057346
更新日期:1985-10-01 00:00:00
abstract::The in vivo activity of two cyclosporins, cyclosporin A (CsA) and a non-immunosuppressive derivative of dihydrocylosporin A (DHCsA-d) against three tapeworms, Hymenolepis microstoma, H. diminuta and Mesocestoides corti, has been assessed. CsA reversibly reduced the dry weight of H. microstoma in the mouse, briefly del...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000062211
更新日期:1989-04-01 00:00:00
abstract::The lung schistosomulum of Schistosoma mansoni is the target of protective immunity in mice singly vaccinated with irradiated cercariae. Since the effector responses are T cell-mediated, their initiation requires the release of antigens from the intact parasite. We have used the technique of biosynthetic labelling wit...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000079245
更新日期:1993-09-01 00:00:00
abstract::The presence of introduced hosts can increase or decrease infections of co-introduced parasites in native species of conservation concern. In this study, we compared parasite abundance, intensity, and prevalence between native Awaous stamineus and introduced poeciliid fishes by a co-introduced nematode parasite (Camal...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182016001001
更新日期:2016-10-01 00:00:00
abstract::Promastigotes of Leishmania braziliensis panamensis absorbed the purines adenine, hypoxanthine, adenosine and inosine by a combination of diffusion and mediated components. When the uptake rates for these substrates were corrected for diffusion and compared, the purine bases adenine and hypoxanthine were transported a...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000055256
更新日期:1982-10-01 00:00:00
abstract::The induction of schistosomulicidal activity of peritoneal macrophages by concanavalin A-stimulated supernatants from long-term T-cell clones and by interferon-gamma (IFN-gamma) was investigated in detail. Optimal conditions of in vitro macrophage activation by T-cell clone supernatants were established. Macrophages f...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s003118200006409x
更新日期:1986-04-01 00:00:00
abstract::Intestinal diarrheagenic polyparasitic infections are among the major public health concerns in developing countries. Here we examined stool specimens by microscopy, DNA dot blot and polymerase chain reaction (PCR) to evaluate the co-infection of four principal protozoans among amoebic dysentery cases from Northeast I...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182015000669
更新日期:2015-09-01 00:00:00
abstract::The effect of different incubation media and of temperature on the induction of water sensitivity has been investigated in intact and tailless Schistosoma mansoni cercariae. Removal of the cercarial tail by vortex stirring and elevation of the temperature of the medium from 27 to 37 degrees C resulted in the rapid ons...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/s0031182000053105
更新日期:1975-08-01 00:00:00
abstract::Eukaryotic 5S rRNA, synthesized by RNA polymerase III (Pol III), is an essential component of the large ribosomal subunit. Most organisms contain hundreds of 5S rRNA genes organized into tandem arrays. However, the genome of the protozoan parasite Leishmania major contains only 11 copies of the 5S rRNA gene, which are...
journal_title:Parasitology
pub_type: 杂志文章
doi:10.1017/S0031182016001712
更新日期:2016-12-01 00:00:00