Dipstick dot ELISA for the detection of Taenia coproantigens in humans.

Abstract:

:A dipstick dot ELISA for detection of Taenia-specific coproantigens was developed. The test was based on a sandwich ELISA using antibodies raised against adult Taenia solium. Antibodies were absorbed to nitrocellulose paper previously adhered to acetate plastic to form dipsticks. Once blocked with 5% skimmed milk and dried the antibody-coated dipsticks were stable for several weeks at room temperature. Both micro and dot ELISA formats were genus specific although the dot ELISA was less sensitive than the micro ELISA based on the same antiserum. During field studies, in which the majority of samples were tested in rural villages soon after collection, 3728 samples were tested. All samples were also examined by microscopy using formol ether concentration and individuals questioned to determine whether they were aware of being infected. After the initial diagnostic work individuals were treated with taeniacidal drugs for worm recovery. Use of the coproantigen test significantly increased the number of cases diagnosed. Of the 41 cases diagnosed by the three diagnostic techniques combined 31 were detected by the dipstick assay making it the most sensitive technique employed. The specificity of the dipstick assay was 99.9% with a positive predictive value of 88.6%. The combined diagnostic approach did not, however, diagnose all cases. The coproantigen test was fast and easy to use. Further improvements may make the dipstick test suitable for wide-scale use in field studies and diagnostic laboratories.

journal_name

Parasitology

journal_title

Parasitology

authors

Allan JC,Mencos F,Garcia-Noval J,Sarti E,Flisser A,Wang Y,Liu D,Craig PS

doi

10.1017/s0031182000079439

subject

Has Abstract

pub_date

1993-07-01 00:00:00

pages

79-85

eissn

0031-1820

issn

1469-8161

journal_volume

107 ( Pt 1)

pub_type

杂志文章
  • Interactions between the plasma proteins of Biomphalaria glabrata (Gastropoda) and the sporocyst tegument of Schistosoma mansoni (Trematoda).

    abstract::The tegumental surface of Schistosoma mansoni sporocysts is the site of both nutritive and immunological interactions with haemolymph cells and plasma of Biomphalaria glabrata, the schistosome intermediate host. Within minutes of being placed in host plasma, sporocysts acquire plasma antigens, and within 3 h host plas...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000065513

    authors: Bayne CJ,Loker ES,Yui MA

    更新日期:1986-06-01 00:00:00

  • Costs of resistance in insect-parasite and insect-parasitoid interactions.

    abstract::Most, if not all, organisms face attack by natural enemies and will be selected to evolve some form of defence. Resistance may have costs as well as its obvious benefits. These costs may be associated with actual defence or with the maintenance of the defensive machinery irrespective of whether a challenge occurs. In ...

    journal_title:Parasitology

    pub_type: 杂志文章,评审

    doi:10.1017/s0031182002001750

    authors: Kraaijeveld AR,Ferrari J,Godfray HC

    更新日期:2002-01-01 00:00:00

  • Gill monogenean communities (Platyhelminthes, Monogenea, Dactylogyridae) of butterflyfishes from tropical Indo-West Pacific Islands.

    abstract::We studied the monogenean communities of 34 species of butterflyfish from the tropical Indo-West Pacific, identifying 13 dactylogyrid species (including two species that are presently undescribed). Monogenean assemblages differed significantly between host species in terms of taxonomic structure, intensity and prevale...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182016001463

    authors: Reverter M,Cutmore SC,Bray R,Cribb TH,Sasal P

    更新日期:2016-10-01 00:00:00

  • The astacin metalloprotease moulting enzyme NAS-36 is required for normal cuticle ecdysis in free-living and parasitic nematodes.

    abstract::Nematodes represent one of the most abundant and species-rich groups of animals on the planet, with parasitic species causing chronic, debilitating infections in both livestock and humans worldwide. The prevalence and success of the nematodes is a direct consequence of the exceptionally protective properties of their ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182010001113

    authors: Stepek G,McCormack G,Birnie AJ,Page AP

    更新日期:2011-02-01 00:00:00

  • What has proteomics taught us about Leishmania development?

    abstract::Leishmania are obligatory intracellular parasitic protozoa that cycle between sand fly mid-gut and phagolysosomes of mammalian macrophages. They have developed genetically programmed changes in gene and protein expression that enable rapid optimization of cell function according to vector and host environments. During...

    journal_title:Parasitology

    pub_type: 杂志文章,评审

    doi:10.1017/S0031182012000157

    authors: Tsigankov P,Gherardini PF,Helmer-Citterich M,Zilberstein D

    更新日期:2012-08-01 00:00:00

  • CYP51 as drug targets for fungi and protozoan parasites: past, present and future.

    abstract::The efficiency of treatment of human infections with the unicellular eukaryotic pathogens such as fungi and protozoa remains deeply unsatisfactory. For example, the mortality rates from nosocomial fungemia in critically ill, immunosuppressed or post-cancer patients often exceed 50%. A set of six systemic clinical azol...

    journal_title:Parasitology

    pub_type: 杂志文章,评审

    doi:10.1017/S0031182018000562

    authors: Lepesheva GI,Friggeri L,Waterman MR

    更新日期:2018-12-01 00:00:00

  • Morphological characterization and molecular phylogeny of zoonotic trematodes in the freshwater snail Asolene platae.

    abstract::In the context of a broader program dealing with the symbiotic associations of apple snails, we sampled three species that coexist in Lake Regatas (Palermo, Buenos Aires, Argentina). The population of Asolene platae, (but not those of Pomacea canaliculata and Pomacea scalaris) showed trematode larvae (echinocercariae ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182019000027

    authors: Dellagnola FA,Montes MM,Martorelli SR,Vega IA

    更新日期:2019-06-01 00:00:00

  • Detection of persistent Plasmodium spp. infections in Ugandan children after artemether-lumefantrine treatment.

    abstract::During a longitudinal study investigating the dynamics of malaria in Ugandan lakeshore communities, a consistently high malaria prevalence was observed in young children despite regular treatment. To explore the short-term performance of artemether-lumefantrine (AL), a pilot investigation into parasite carriage after ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S003118201400033X

    authors: Betson M,Sousa-Figueiredo JC,Atuhaire A,Arinaitwe M,Adriko M,Mwesigwa G,Nabonge J,Kabatereine NB,Sutherland CJ,Stothard JR

    更新日期:2014-12-01 00:00:00

  • Detection of eight Cryptosporidium genotypes in surface and waste waters in Europe.

    abstract::Cryptosporidia are important protozoan parasites of vertebrates, and a number of species and genotypes, with different host ranges, have been described. In this study a protocol was established for the detection and the genetic characterization of Cryptosporidium spp. isolated from various types of surface waters (riv...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182001001317

    authors: Ward PI,Deplazes P,Regli W,Rinder H,Mathis A

    更新日期:2002-04-01 00:00:00

  • Mice infected with the larvae of Taenia crassiceps exhibit a Th2-like immune response with concomitant anergy and downregulation of Th1-associated phenomena.

    abstract::Infection of intermediate hosts with eggs of taeniid parasites results in a larval infestation known as cysticercosis. A number of studies have indicated that cysticercosis is associated with immunosuppression, although little is known about the mechanisms involved. In the present study, mice infected with the larvae ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000066142

    authors: Villa OF,Kuhn RE

    更新日期:1996-06-01 00:00:00

  • Biological control of ticks.

    abstract::Ticks have numerous natural enemies, but only a few species have been evaluated as tick biocontrol agents (BCAs). Some laboratory results suggest that several bacteria are pathogenic to ticks, but their mode of action and their potential value as biocontrol agents remain to be determined. The most promising entomopath...

    journal_title:Parasitology

    pub_type: 杂志文章,评审

    doi:10.1017/s0031182004005219

    authors: Samish M,Ginsberg H,Glazer I

    更新日期:2004-01-01 00:00:00

  • Four new species of KudoaMeglitsch, 1947 (Myxosporea: Multivalvulida) from Australia with recommendations for species descriptions in the Kudoidae.

    abstract::Kudoid parasites are significant pathogens of marine fish. In the past, specific identification has been difficult due to a paucity of detailed morphological and biological information provided in the original description of some species. However, the introduction of DNA analysis has dramatically improved diagnosis. F...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182009991557

    authors: Burger MA,Adlard RD

    更新日期:2010-04-01 00:00:00

  • Axenic cultivation and partial characterization of Leishmania braziliensis amastigote-like stages.

    abstract::Leishmania braziliensis strain M2903 was adapted for growth and serially maintained as amastigotes at 34 degrees C in modified UM-54 medium, with growth curves exhibiting typical log and stationary phases. In late passages, amastigote growth took place in the absence of supplementary haemin and was unaffected when the...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s003118209700214x

    authors: Balanco JM,Pral EM,da Silva S,Bijovsky AT,Mortara RA,Alfieri SC

    更新日期:1998-02-01 00:00:00

  • Phagocyte-specific S100 proteins in the local response to the Echinococcus granulosus larva.

    abstract::Infection by larval Echinococcus granulosus is usually characterized by tight inflammatory control. However, various degrees of chronic granulomatous inflammation are also observed, reaching a high point in infection of cattle by the most prevalent parasite strain worldwide, which is not well adapted to this host spec...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S003118201100179X

    authors: Basika T,Muñoz N,Casaravilla C,Irigoín F,Batthyány C,Bonilla M,Salinas G,Pacheco JP,Roth J,Durán R,Díaz A

    更新日期:2012-02-01 00:00:00

  • Mechanisms underlying reduced expulsion of a murine nematode infection during protein deficiency.

    abstract::Balb/c mice infected with the gastrointestinal nematode Heligmosomoides bakeri were fed protein sufficient (PS, 24%) or deficient (PD, 3%) diets to investigate whether diet, infection or dose of larval challenge (0, 100 or 200 larvae) influenced gut pathophysiology and inflammation. Among the PS mice, worms were more ...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182007003617

    authors: Tu T,Koski KG,Scott ME

    更新日期:2008-01-01 00:00:00

  • Glasgow encounters with tropical diseases.

    abstract::The period 1875-1925 was remarkable in the history of parasitology mainly for the elucidation of the life cycles of parasites causing important parasitic diseases and the incrimination of vectors in their transmission. These discoveries were made by a small number of scientists working in the tropics a number of whom ...

    journal_title:Parasitology

    pub_type: 社论,历史文章

    doi:10.1017/S0031182017000956

    authors: Cox FEG

    更新日期:2017-10-01 00:00:00

  • Efficacy of narasin against hepatic and intestinal coccidiosis in rabbits.

    abstract::The anticoccidial effect of narasin against Eimeria flavescens, E. intestinalis, E. magna, E. perforans and E. stiedai was tested in experimentally infected rabbits. The drug was highly effective in reducing oocyst output and in preventing clinical signs. Maximum activity as measured by oocyst discharge was achieved w...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000085309

    authors: Peeters JE,Geeroms R,Antoine O,Mammerickx M,Halen P

    更新日期:1981-10-01 00:00:00

  • Current status of vaccination against African trypanosomiasis.

    abstract::Anti-trypanosomiasis vaccination still remains the best theoretical option in the fight against a disease that is continuously hovering between its wildlife reservoir and its reservoir in man and livestock. While antigenic variation of the parasite surface coat has been considered the major obstacle in the development...

    journal_title:Parasitology

    pub_type: 杂志文章,评审

    doi:10.1017/S0031182010000223

    authors: Magez S,Caljon G,Tran T,Stijlemans B,Radwanska M

    更新日期:2010-12-01 00:00:00

  • The schistosome in the mammalian host: understanding the mechanisms of adaptation.

    abstract::In this review, we envisage the host environment, not as a hostile one, since the schistosome thrives there, but as one in which the relationship between the two organisms consists of constant communication, through signalling mechanisms involving sense organs, surface glycocalyx, surface membrane and internal organs ...

    journal_title:Parasitology

    pub_type: 杂志文章,评审

    doi:10.1017/S0031182007002971

    authors: Kusel JR,Al-Adhami BH,Doenhoff MJ

    更新日期:2007-10-01 00:00:00

  • A global sensitivity analysis for African sleeping sickness.

    abstract::African sleeping sickness is a parasitic disease transmitted through the bites of tsetse flies of the genus Glossina. We constructed mechanistic models for the basic reproduction number, R0, of Trypanosoma brucei gambiense and Trypanosoma brucei rhodesiense, respectively the causative agents of West and East African h...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182010001496

    authors: Davis S,Aksoy S,Galvani A

    更新日期:2011-04-01 00:00:00

  • Low levels of transforming growth factor-beta (TGF-beta) and reduced suppression of Th2-mediated inflammation in hyperreactive human onchocerciasis.

    abstract::Th2-biased inflammation with eosinophilia and IgE production is a hallmark of helminth infections. It is pronounced in hyperreactive onchocerciasis patients ('sowda' or 'local form'), who efficiently kill microfilariae resulting in severe dermatitis and lymphadenitis. In contrast, hyporeactive patients ('generalised f...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182010000922

    authors: Korten S,Hoerauf A,Kaifi JT,Büttner DW

    更新日期:2011-01-01 00:00:00

  • Physiological cost induced by the maternally-transmitted endosymbiont Wolbachia in the Drosophila parasitoid Leptopilina heterotoma.

    abstract::Endosymbiotic bacteria of the genus Wolbachia infect a number of invertebrate species in which they induce various alterations in host reproduction, mainly cytoplasmic incompatibility (CI). In contrast to most other maternally transmitted parasites, manipulation of host reproduction makes the spread of Wolbachia possi...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182099006599

    authors: Fleury F,Vavre F,Ris N,Fouillet P,Boulétreau M

    更新日期:2000-11-01 00:00:00

  • Immunolocalization of Taenia solium gap junction innexins.

    abstract::In previous studies, ultrastructural observations revealed a large number of gap junctions (GJs) in the neck and immature proglottid tissues of Taenia solium tapeworms. In these helminths, cytoplasmic glycogen sacs are connected by numerous discrete GJs to other cells throughout the maturing strobilar tissue. Disconti...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182008004629

    authors: Zurabian R,Landa A,Robert L,Willms K

    更新日期:2008-08-01 00:00:00

  • Differentiation of Schistosoma mansoni from S. rodhaini using cloned DNA probes.

    abstract::The ribosomal RNA (rRNA) gene units of Schistosoma mansoni and S. rodhaini, of the lateral spined egg group, have been studied. The schistosome rRNA gene unit consists of a regular interspersion of the two genes encoding the large and small rRNA units with two spacers. The large spacer is not transcribed while the sma...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000059709

    authors: Walker TK,Simpson AJ,Rollinson D

    更新日期:1989-02-01 00:00:00

  • Cytosolic glutathione S-transferases of Oesophagostomum dentatum.

    abstract::Oesophagostomum dentatum stages were investigated for glutathione S-transferase (GST) expression at the protein and mRNA levels. GST activity was detected in all stages (infectious and parasitic stages including third- and fourth-stage larvae of different ages as well as males and females) and could be dose-dependentl...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182008004769

    authors: Joachim A,Ruttkowski B

    更新日期:2008-09-01 00:00:00

  • Proteomic analysis of Entamoeba histolytica.

    abstract::In this study, the proteome of axenically grown Entamoeba histolytica parasites was explored by two-dimensional gel electrophoresis (2-DE), employing a practical and effective procedure for the solubilization of E. histolytica proteins. Approximately 900 protein species in the pH range between 4 and 7 were detected by...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182006001442

    authors: Tolstrup J,Krause E,Tannich E,Bruchhaus I

    更新日期:2007-02-01 00:00:00

  • Synergistic antimalarial activity of ketones with rufigallol and vitamin C.

    abstract::Malaria remains a major cause of human morbidity and mortality worldwide. Plasmodium falciparum, the most virulent of the 4 human Plasmodium species causing malaria, is potentially life threatening, is increasing in prevalence and is becoming even more resistant to in-use drugs. In light of the growing problem of mult...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/S0031182005008267

    authors: Mahajan SS,Kamath VR,Ghatpande SS

    更新日期:2005-10-01 00:00:00

  • In vitro cultivation of Trypanosoma congolense bloodstream forms in the absence of feeder cell layers.

    abstract::Bloodstream forms of Trypanosoma congolense (2 clones: ILNat3.1 and IL3000, and 4 stocks: IL2079, IL2466, IL3266 and CP-81) were continuously cultivated in vitro at 34-36 degrees C in the absence of feeder cell layers, using HMI-93 medium which was modified from Iscove's modified Dulbecco's MEM (Flow Laboratories, Irv...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000062533

    authors: Hirumi H,Hirumi K

    更新日期:1991-04-01 00:00:00

  • The diagnosis of malaria infection using a solid-phase radioimmunoassay for the detection of malaria antigens. Application to the detection of Plasmodium berghei infection in mice.

    abstract::A method has been devised to show that malaria parasites can be detected serologically in infected blood with a high degree of sensitivity. Using a murine malaria model, parasites were demonstrated in a solid-phase radio-immunoassay which measured antibody-binding inhibition. Lysed red blood cells (r.b.c.) were incuba...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000000639

    authors: Mackey L,Perrin L,Leemans E,Lambert PH

    更新日期:1980-02-01 00:00:00

  • Asparaginyl endopeptidase activity in adult Schistosoma mansoni.

    abstract::Sequence comparisons have recently shown that the Schistosoma mansoni protein Sm32 is similar to asparaginyl endoproteinases, a novel family of cysteine proteinases, of which the legumains from legumes are the best characterized. By synthesizing and employing fluorogenic peptide substrates for the specific detection o...

    journal_title:Parasitology

    pub_type: 杂志文章

    doi:10.1017/s0031182000077052

    authors: Dalton JP,Hola-Jamriska L,Brindley PJ

    更新日期:1995-12-01 00:00:00