Molecular characterization of 5S ribosomal RNA genes and transcripts in the protozoan parasite Leishmania major.

Abstract:

:Eukaryotic 5S rRNA, synthesized by RNA polymerase III (Pol III), is an essential component of the large ribosomal subunit. Most organisms contain hundreds of 5S rRNA genes organized into tandem arrays. However, the genome of the protozoan parasite Leishmania major contains only 11 copies of the 5S rRNA gene, which are interspersed and associated with other Pol III-transcribed genes. Here we report that, in general, the number and order of the 5S rRNA genes is conserved between different species of Leishmania. While in most organisms 5S rRNA genes are normally associated with the nucleolus, combined fluorescent in situ hybridization and indirect immunofluorescence experiments showed that 5S rRNA genes are mainly located at the nuclear periphery in L. major. Similarly, the tandemly repeated 5S rRNA genes in Trypanosoma cruzi are dispersed throughout the nucleus. In contrast, 5S rRNA transcripts in L. major were localized within the nucleolus, and scattered throughout the cytoplasm, where mature ribosomes are located. Unlike other rRNA species, stable antisense RNA complementary to 5S rRNA is not detected in L. major.

journal_name

Parasitology

journal_title

Parasitology

authors

Moreno-Campos R,Florencio-Martínez LE,Nepomuceno-Mejía T,Rojas-Sánchez S,Vélez-Ramírez DE,Padilla-Mejía NE,Figueroa-Angulo E,Manning-Cela R,Martínez-Calvillo S

doi

10.1017/S0031182016001712

subject

Has Abstract

pub_date

2016-12-01 00:00:00

pages

1917-1929

issue

14

eissn

0031-1820

issn

1469-8161

pii

S0031182016001712

journal_volume

143

pub_type

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